Regulators
Hexokinase is regulated by its products: ADP and glucose-6-phosphate. Hexokinase is allosterically inhibited by glucose-6-phosphate.
Glucokinase is activated by un-phosphorylated phosphofructokinase 2 and fructose-2,6-bisphosphate. They keep glucokinase in the cytoplasm and active.
Phosphofructokinase is inhibited by ATP and citrate. (See 10/10/18 lecture at around 20 minutes). Phosphofructokinase is activated by ADP, AMP (AKA adenylate), and fructose-2,6-bisphosphate.
Pyruvate kinase is inhibited allosterically by ATP. Pyruvate kinase is inhibited by alanine. Pyruvate kinase is inhibited by phosphorylation (using PKA). Pyruvate kinase is dependent on lots of fructose-1,6-bisphosphate (pyruvate kinase can “see” what phosphofructokinase is doing; if phosphofructokinase is v. active and making a lot of fructose 1,6-bisphosphate, that’s “telling” pyruvate kinase to keep making pyruvate, which you don’t want if you’re trying to inhibit pyruvate kinase)
