MITOCHONDRIA * Generate energy for the cell * Have numerous metabolic functions * Comprise mitochondrial proteins (most encoded by nuclear genes) * Compartmental organization: i. Outer membrane ii. Intermembrane space iii. Inner membrane iv. Matrix

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MITOCHONDRIA * Generate energy for the cell * Have numerous metabolic functions * Comprise mitochondrial proteins (most encoded by nuclear genes) * Compartmental organization: i. Outer membrane ii. Intermembrane space iii. Inner membrane iv. Matrix
KEY MECHANISMS • Cotranslational: ribosomes continue synthesizing protein as it crosses membrane • Post-translational: protein imports after it is completely synthesized by cytosolic ribosomes COTRANSLATIONAL IMPORT OF WATER-SOLUBLE PROTEINS 1. S