The main goal of this research was to investigate the diversity of lignocellulolytic bacteria in the native soil of sugarcane trash and to assess their biodegradation potential. From naturally degrading sugarcane waste, bacteria were identified. 35 distinct bacterial species were discovered; these bacteria were then employed to determine each one’s potential for breaking down the lignin and cellulose found in sugarcane waste. After selecting a potential strain of bacteria, Congo red and iodine tests were created for the purpose of screening bacterial species, and they were then utilized to further the biodegradation of sugarcane waste. Thirteen of the thirty-five investigated bacterial species generated cellulase and ligninase enzymes, and Pseudomonas fluorescens was identified in the secondary screening as a suitable strain among these. Hence, this P. fluorescens was employed in the degradation of sugarcane waste. When this lingo-cellulolytic bacterium was introduced to sugarcane waste, the rate of degradation of the waste was enhanced. Significant reduction in lignin and cellulose contents were observed in sugarcane trash inoculated with P. fluorescens compared to other experiments. P. fluorescens lowered the C:N ratio in soil-mixed sugarcane waste from 70:1 to 10:1. The macronutrients of the compost taken from experimental trays seeded with P. fluorescens showed a substantial increase as well. It is evident from these results that the P. fluorescens, lingo-cellulolytic bacteria may be used for the degradation of sugarcane trash. Hence, we conclude that P. fluorescens can be recommended for the degradation of sugarcane trash which would result in the production of good quality compost containing higher amounts of total nitrogen, total potassium and total phosphorus contents.
