#restriction digest

[coma-adult]

Restriction mapping

- or how i like to call it:

"letting everybody know that you don't have any intuition for numbers and ratios"

[expbio-blog]

The following is a

Start setting up restriction digest reaction with NdeI and BamHI so pREP1, pREP41, and pREP81 are linearized.

[molecularbiologistproblems]

Molecular Biologist Tip! I recommend com.whiz.nebtools v 1.2. Visit the links from your android device. com.whiz.nebtools v 1.2 ( http://market.android.com/search?q=pname:com.whiz.nebtools ) This is a great tool when it comes to cloning genes! If you’re in the lab and need to do a restriction digest for whatever reason, just look up the restriction enzyme on this app. It will tell you what conditions to perform the restriction digest at and even will compare optimal reaction conditions for double digests! If you go to New England Biolab’s (NEB) website (www.neb.com), they have a great “cutter” tool, which will help you decide what restriction enzymes are the best matches for your cloning/ligation reaction. Just use NCBI’s database to look up the GenBank number and it will pull the sequence up for you! More user-friendly bioinformatic tips are coming! Please ask a question if you would like assistance with something in particular!!!

[myhalohalo]

Corpus Christi weather

the weather here is a fickle bitch sometimes, to be quite honest.

Yesterday was GORGEOUS. The sun was out all afternoon, the temperature was mild, and there was a nice breeze. Spent time with my mama outside; I mowed the lawn while she tended to her plants. It was wonderful.

Today, it is gloomy and has been sprinkling on and off all morning. Normally I like to have a gloomy movie day every now and then, but not today. It puts me in a lazy mood when I need to be productive today and finish my lab work. -__- (On that note, if someone knows how to plot on semi-log graph paper a calibration plot for a restriction digest lab using gel electrophoresis, PLEASE HALPPP. I'm quite confused)

Anyhoo. Enough whining. I am just procrastinating. Metallica playlist on deck, ready to do work.

Have an amazing day, my lovelies! (:

[stanfordbrownigem2012]