Mar 21, 2013
New Ask Taqman Video: Finding Your Sensitive Side in Real-time PCR
Researchers who study liver disease in hippopotamus should count themselves fortunate; they never run low on RNA for their gene expression studies. But what about those who work on micro-dissected mouse tumor samples, even single cells? Or who work on limited cell-sorted fractions? Or researchers like Andre of the Barretos Cancer Hospital, who shared the following concern: “I'm studying a mutation in a specific gene that’s being transcribed at extremely low levels in human cells. What can I do to improve qPCR reaction sensitivity of my real-time assay?” Watch a video reply to his question... Watch Video