Mike Driver
Acquired Stardust
d e v o n

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I'd rather be in outer space ๐ธ
Keni
YOU ARE THE REASON
Game of Thrones Daily
art blog(derogatory)

็ฅๆฅ / Permanent Vacation

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Today's Document
Alisa U Zemlji Chuda
Cosimo Galluzzi

โฃ Chile in a Photography โฃ
he wasn't even looking at me and he found me
2025 on Tumblr: Trends That Defined the Year

ellievsbear
Peter Solarz

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@burnmemos
Human texts could long outlast the human race with a new digital storage system.
In 2013, the researchers recorded a 300-kilobyte text file, but the capacity of a single glass data disc is 360 terabytes (1,000,000,000 kilobytes), they reported. The discs are stable up to 1,832 degrees F (1,000 degrees C).
Southampton researchers gave the copy of the Universal Declaration of Human Rights as a gift to the United Nations Educational Scientific and Cultural Organization (UNESCO) in early February, part of the closing ceremony of that organization's Year of Light initiative. The scientists call the glass the "Superman memory crystal," a nod to memory crystals in the "Superman" films and comics. According to the university, the researchers are now seeking industry partners to further develop the technology.
This presentation is part of the 'Brain Science and Effective Leadership Series,' hosted by the Stockdale Center for Ethical Leadership. Dr. Girordano is wit...
Weโve all heard of mind over matter, but what about military over mind? Or vice versa? Hereโs the US Naval Academy presentation with Dr. James Giordano, PhD, on Neurocognitive Science in the military. Further reading: https://www.usna.edu/NewsCenter/sites/Ethics/Dr._James_Giordano_Battlescape_Brain_Military_and_Intelligence_Use_of_Neurocognitive_Science.php
Animations of research on Lissajou aka Bowditch figures by Wolfgang W. Daeumler on particle physics patterns represented by oscillations of horn torus (akaย โdonutโ) field geometry. Daeumler writes,ย โ[The] digits stand for direction of spin (rotation around axis) / direction of time (revolution around horn torus bulge) / parity (particular inverse trajectory ;-) 000 ย 001 ย 010 ย 011ย 100 ย 101 ย 110 ย 111ย โ โ left two columns symbolise [sic] particles in forward direction of time, right two columns antiparticles (reversed time), ย columns 1,3: parity 'right' (0), columns: 2,4: parity 'left' (1)
Unbelievable these lawyers say "We know these alleged victims are minors, but we'd like to call them "ACCUSERS" and not allow their lawyers to call them victims. #GhislaineMaxwell #DontSuicide #EpsteinIsland https://t.co/ON8QZN5nvx
Unbelievable these lawyers say "We know these alleged victims are minors, but we'd like to call them "ACCUSERS" and not allow their lawyers to call them victims. #GhislaineMaxwell #DontSuicide #EpsteinIsland https://t.co/ON8QZN5nvx
โ @BurnhamMemos ๐บ๐ฒ๐ต๐ช๐ฉ๐ช๐จ๐ฎ๐บ๐ธ (@JimmyMillenial) Nov 6, 2021
from Twitter https://twitter.com/JimmyMillenial November 06, 2021 at 07:01PM via IFTTT
Are humans a cancer to the planet?
Are humans a cancer to the planet?
โ @BurnhamMemos ๐บ๐ฒ๐ต๐ช๐ฉ๐ช๐จ๐ฎ๐บ๐ธ (@JimmyMillenial) Nov 6, 2021
from Twitter https://twitter.com/JimmyMillenial November 06, 2021 at 06:59PM via IFTTT
RT @DegreaseNeil: Physics fun https://t.co/NsP6YE2L5W
RT @DegreaseNeil: Physics fun https://t.co/NsP6YE2L5W
โ @BurnhamMemos ๐บ๐ฒ๐ต๐ช๐ฉ๐ช๐จ๐ฎ๐บ๐ธ (@JimmyMillenial) Oct 31, 2021
from Twitter https://twitter.com/JimmyMillenial October 31, 2021 at 07:47PM via IFTTT
Stay spooky, humans ๐๐ #HappyHalloween2021 https://t.co/dQ4MeUps3e https://t.co/yPXldmJddh
Stay spooky, humans ๐๐ #HappyHalloween2021 https://t.co/dQ4MeUps3e https://t.co/yPXldmJddh
โ @BurnhamMemos ๐บ๐ฒ๐ต๐ช๐ฉ๐ช๐จ๐ฎ๐บ๐ธ (@JimmyMillenial) Oct 31, 2021
from Twitter https://twitter.com/JimmyMillenial October 31, 2021 at 05:52PM via IFTTT
President God will one day create the best President that God ever created. Until then, peace & love with us all.
President God will one day create the best President that God ever created. Until then, peace & love with us all.
โ @BurnhamMemos ๐บ๐ฒ๐ต๐ช๐ฉ๐ช๐จ๐ฎ๐บ๐ธ (@JimmyMillenial) Oct 29, 2021
from Twitter https://twitter.com/JimmyMillenial October 29, 2021 at 07:56PM via IFTTT
RT @disclosetv: JUST IN - Vatican abruptly cancels live broadcast of Biden meeting Pope without explanation (AP)
RT @disclosetv: JUST IN - Vatican abruptly cancels live broadcast of Biden meeting Pope without explanation (AP)
โ @BurnhamMemos ๐บ๐ฒ๐ต๐ช๐ฉ๐ช๐จ๐ฎ๐บ๐ธ (@JimmyMillenial) Oct 29, 2021
from Twitter https://twitter.com/JimmyMillenial October 29, 2021 at 07:49PM via IFTTT
Anyone in #VA notify your neighbors near any shopping centers or mall to look out for suspicious activity #virginia #woodbridge #alexandria #AlexandriaVA #NeighborhoodWatch
Anyone in #VA notify your neighbors near any shopping centers or mall to look out for suspicious activity #virginia #woodbridge #alexandria #AlexandriaVA #NeighborhoodWatch
โ @BurnhamMemos ๐บ๐ฒ๐ต๐ช๐ฉ๐ช๐จ๐ฎ๐บ๐ธ (@JimmyMillenial) Oct 29, 2021
from Twitter https://twitter.com/JimmyMillenial October 29, 2021 at 07:36PM via IFTTT
Alexandria / Woodbridge area(s) of northern Virginia are under alert for potential ISIS attack
Alexandria / Woodbridge area(s) of northern Virginia are under alert for potential ISIS attack
โ @BurnhamMemos ๐บ๐ฒ๐ต๐ช๐ฉ๐ช๐จ๐ฎ๐บ๐ธ (@JimmyMillenial) Oct 29, 2021
from Twitter https://twitter.com/JimmyMillenial October 29, 2021 at 07:24PM via IFTTT
Cool Democrats Create Liberation In Everything Simultaneously
Cool Democrats Create Liberation In Everything Simultaneously
โ @BurnhamMemos ๐บ๐ฒ๐ต๐ช๐ฉ๐ช๐จ๐ฎ๐บ๐ธ (@JimmyMillenial) Oct 29, 2021
from Twitter https://twitter.com/JimmyMillenial October 29, 2021 at 06:58PM via IFTTT
SARS-CoV-2 from Patient with COVID-19, USA
Whole-Genome Sequencing
We designed 37 pairs of nested PCRs spanning the genome on the basis of the coronavirus reference sequence (GenBank accession no. NC045512). We extracted nucleic acid from isolates and amplified by using the 37 individual nested PCRs. We used positive PCR amplicons individually for subsequent Sanger sequencing and also pooled them for library preparation by using a ligation sequencing kit (Oxford Nanopore Technologies, https://nanoporetech.comExternal Link), subsequently for Oxford Nanopore MinION sequencing. We generated consensus nanopore sequences by using Minimap version 2.17 (https://github.comExternal Link) and Samtools version 1.9 (http://www.htslib.orgExternal Link). We generated consensus sequences by Sanger sequencing from both directions by using Sequencher version 5.4.6 (https://www.genecodes.comExternal Link), and further confirmed them by using consensus sequences generated from nanopore sequencing.
To sequence passage 4 stock, we prepared libraries for sequencing by using the Next Ultra II RNA Prep Kit (New England Biolabs, https://www.neb.comExternal Link) according to the manufacturerโs protocol. In brief, we fragmented โ70โ100 ng of RNA for 15 min, followed by cDNA synthesis, end repair, and adaptor ligation. After 6 rounds of PCR, we analyzed libraries by using an Agilent Bioanalyzer (https://www.agilent.comExternal Link) and quantified them by using a quantitative PCR. We pooled samples and sequenced samples by using a paired-end 75-base protocol on an Illumina (Illumina, Inc., https://www.illumina.comExternal Link) MiniSeq instrument and using the High-Output Kit and then processed reads by using Trimmomatic version 0.36 (11) to remove low-quality base calls and any adaptor sequences. We used the de novo assembly program ABySS (12) to assemble the reads into contigs by using several different sets of reads and kmer values ranging from 20 to 40. We compared contigs >400 bases against the National Center for Biotechnology Information (Bethesda, MD, USA) nucleotide collection using BLAST (https://blast.ncbi.nlm.nih.govExternal Link). A nearly full-length viral contig obtained in each sample had 100% identity to the 2019-nCoV/USA-WA1/2020 strain (GenBank accession no. MN985325.1). All the remaining contigs mapped to either host cell rRNA or mitochondria. We mapped the trimmed reads to the reference sequence by using BWA version 0.7.17 (13) and visualized these reads by using the Integrated Genomics Viewer (14) to confirm the identity with the USA-WA1/2020 strain.
Widespread adoption of massively parallel deoxyribonucleic acid (DNA) sequencing instruments has prompted the recent development of de novo short read assembly algorithms. A common shortcoming of the available tools is their inability to efficiently assemble vast amounts of data generated from large โฆ
Genome Res
. 2009 Jun;19(6):1117-23. doi: 10.1101/gr.089532.108. Epub 2009 Feb 27.ABySS: a parallel assembler for short read sequence data
Jared T Simpson
1
,
Kim Wong
,
Shaun D Jackman
,
Jacqueline E Schein
,
Steven J M Jones
,
Inanรง Birol
Affiliations expand
PMID: 19251739
PMCID: PMC2694472
DOI: 10.1101/gr.089532.108
Free PMC article
Abstract
Widespread adoption of massively parallel deoxyribonucleic acid (DNA) sequencing instruments has prompted the recent development of de novo short read assembly algorithms. A common shortcoming of the available tools is their inability to efficiently assemble vast amounts of data generated from large-scale sequencing projects, such as the sequencing of individual human genomes to catalog natural genetic variation. To address this limitation, we developed ABySS (Assembly By Short Sequences), a parallelized sequence assembler. As a demonstration of the capability of our software, we assembled 3.5 billion paired-end reads from the genome of an African male publicly released by Illumina, Inc. Approximately 2.76 million contigs > or =100 base pairs (bp) in length were created with an N50 size of 1499 bp, representing 68% of the reference human genome. Analysis of these contigs identified polymorphic and novel sequences not present in the human reference assembly, which were validated by alignment to alternate human assemblies and to other primate genomes.
#DownGoesMaxwell #USvMaxwell #MaxwellTrial https://t.co/mnbQLJrZux https://t.co/Y9KMkl39yG
#DownGoesMaxwell #USvMaxwell #MaxwellTrial https://t.co/mnbQLJrZux https://t.co/Y9KMkl39yG
โ @BurnhamMemos ๐บ๐ฒ๐ต๐ช๐ฉ๐ช๐จ๐ฎ๐บ๐ธ (@JimmyMillenial) Oct 19, 2021
from Twitter https://twitter.com/JimmyMillenial October 19, 2021 at 01:53PM via IFTTT
An online planetarium from In-The-Sky.org, showing what stars and planets you'll be able to see in the night sky on any given day of the year.
Awesome resources including a 3D system of the main planetary bodies around our sun