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Liquid Chromogenic Culture Media

Liquid Chromogenic Culture Medium

Coliforms and E. coli liquid chromogenic medium

Performance Characteristics of Liquid Chromogenic Culture Media

1. It is included in the "Japan Food Hygiene Guide" (2004 edition) with strong authoritative influence.

2. There are two kinds of chromogenic substances, which can directly recognize coliforms and E. coli.

3. The test results can be obtained within 24 hours, but BGLB needs 48 hours.

4. No need for a fermentation tube and a constant temperature pool with low cost and easy operation.

5. No need to prepare medium and wash tubes (except XM powder medium).

6. No need for identification and supplementation experiments such as EMB culture.

7. E.coli O157 cannot be identified separately but qualitative testing only.

Product Details of Liquid Chromogenic Culture Media

Name

Model

Application

Capacity

Packing

Validity Period

Liquid chromogenic culture medium

XM-30

For 1ml sample inspection

10ml/cup

128 cups/carton

1 year

Liquid chromogenic culture medium

XM-31

For 10ml sample inspection

10ml/cup

128 cups/carton

Liquid chromogenic culture medium

XM-32

For 5ml sample inspection

5ml/cup

128 cups/carton

XM powder medium

XM0001

For preparing medium

8.5g/bag

20 bags/carton

3 years

MT opener

OS-01

For MT container opening

/

1pc

/

Usage Method of Liquid Chromogenic Culture Media

1. Container surface sterilization: The surface of the aluminum foil should be disinfected with an alcohol lamp before use.

2. Opening: Open the aluminum foil with an MT opener (OS-01) or open directly with a sterile pipette.

3. Inject the sample diluent: use a pipette to inject the sample dilution into the cup

XM-30: 1ml, XM-31: 10ml, XM-32: 5ml

4. Cultivation: Culture time: 24 hours; Culture temperature: 36 ± 1 °C

5. Disposal: Dispose of them after sterilization

※XM powder medium usage: Dissolve 500ml of pure water per bag, inject 10ml into the test tube, autoclave, then inject 1ml sample, and incubate at 36±1°C for 24 hours. The identification method is the same.

Coloring Principle

Coliforms positive

There is a special β-galactosidase only exit in the coliforms. It can be used as an indicator enzyme for coliform bacteria, which catalyzes the hydrolysis of the substrate X-GAL to form 5,5-dibromo -4,4-dichloro indigo, appearing light blue ~ purple-blue.

E. coli positive

β--glucuronidase can be used as an indicator enzyme for Escherichia coli; because β-glucuronidase can be observed in Escherichia coli, Salmonella and Shigella in intestinal bacteria, and 95% of E. coli contains β-glucuronidase. It catalyzes the hydrolysis of MUG to form 4-methylumbelliferon, which exhibits fluorescence at 366 nm ultraviolet light and is visually discernible under indoor light intensity.

https://www.elexbio.com/liquid-chromogenic-culture-media.html

#chromogenic #culture

Homogenizer

Performance Characteristics of Homogenizer

1. The exclusive design of the crushing plate adopts a hard rubber blade that has a strong crushing ability. The smashing takes a short time, which is about half of the conventional method.

2. Stainless steel material is used. The sample has no contact with the laboratory homogenizer, and the packaging is hygienic and safe.

3. The mobile glass door switch is easy to load and unload, and the smashing state of the sample can be directly observed through a transparent window.

4. The instrument can be used with our dispensing pump to fully mix the diluted sample.

5. There are manual mode and an automatic mode, and the smashing time can be easily set.

Crushing Ability Comparison

Conventional flat bats are difficult to completely smash the sample, and the new pulverized plate with a hard rubber blade can completely smash the sample in a short time.

Functional application

1. The instrument is suitable for sample preparation of microbial analysis of various foods, medicines, and cosmetics.

2. Put the substance to be tested into the blender bag and inject the dilution solution (our company's dispensing pump is suitable for solvent diluent ), close the glass door, the pulverizing plate of the homogenizer will start to slap, the pressure generated will crush and mix the contents of the bag.

3. Specification of Homogenizer

4. Name

5. Model

6. Specification

7. Validity Period

8. Homogenizer

9. Pro•media SH-ⅡM

10. Power supply: AC110V

11. 1 year

12. Power: 100W

13. Weight: 17KGS

14. Dimension: 255*345*345mm

15. Automatic mode: 15s, 30s, 30s

16. Manual mode: automatic shutdown after 10 minutes of continuous operation

17. The matching use of the company's homogenized bag series is more effective.

https://www.elexbio.com/homogenizer.html

#lab #homogenizers

Dispensing Pump

Performance Characteristics of Dispensing Pump

1. Small size, light design, easy to clean and move.

2. According to the operation needs, the nozzle can be installed freely left and right.

3. The pneumatic type liquid feeding method is adopted, and the dispensing speed is increased by 20% compared with the original product.

4. The dilution solution can use our company's sterilization diluents MV2-1000PBS or filling the homemade dilution solution into the matching sterilization bottle SB4000 to contact the device.

5. Intelligent mode, automatic dilution: automatically measure the sample weight and multiple dilute the sample.

There is no need to manually measure the sample weight.

6. Quantitative dispensing: The packing amount can be set freely.

7. Choose to have a printer and connect to a computer for data management.

8. The specific dilution factor will be automatically archived for the next dilution.

9. Stainless steel material is easy to clean. The pedal switch can be used in a clean stand.

10. Low noise. It can be stopped quickly and no residual droplets.

Technical Specifications of Dispensing Pump

Sample weight

0.5~1000g

Dilution amount

5~1000ml

Dilution precision

Within ±1.0g

Dilution speed

16.67ml/sec (6 seconds for 100ml dispensing)

Dilution multiple

2-999 times

Weighing range

0.1~1500g

Density setting

0.7-1.4g/ml

Material

Stainless steel

Power supply

AC 110V

Weight

7.5kg

Dimension

320х280х270mm

Products Details of Dispensing Pump

Products List

Selective Purchasing products

Name

QTY

Name

Model

QTY

Warranty period

Dispensing pump

1 set

Printers (heat sensitive photocopiers, wiring, AC presses, heat sensitive paper)

PA030

1

1 year

Dispensing nozzle

1set

Computer Output Devices (Special Software, RS232C Wiring)

PA061

1

Silicone gel tube

2 pcs

Pedal switch

PA037

1

Electronic scale

1 set

4 liter sterilizing bottle, cap for injection/extraction, circular filter plate, silica gel tube (nozzle, infusion tube can be sterilized together after connecting)

SB4000

1 set

DT-Cube bracket（SUS）

1 set

MV2-1000 infusion apparatu

1 set

Sterilization diluents (phosphate buffered saline, 1 liter х10 bottles)

MV2-1000

1 set

For homogenizers, please refer to ELMEX's Pro•media SH-IIM.

It is better to use the company's diluents and homogenized blender bag series.

https://www.elexbio.com/dispensing-pump.html

#culture #media

Diluents Series

In daily practical experiments, when the liquid samples are collected correctly, we often do not need a pure solution to carry out experiments. In order to get better and more accurate detection results, diluent water or other solutions will be added to obtain the solution with lower concentration. In this process, the solvent diluent is needed. In many laboratories, it is generally believed that direct preparation of buffer by diluent reagent will be more accurate and cheaper, which is not in line with the fact, because the quality of buffer is related to the purity of reagent, the accuracy of reagent weighing, the quality of purified water and other factors such as temperature, preparation container and so on.

ELEX diluents series has greatly improved the trouble and inaccuracy caused by the preparation of diluents on site. The product mainly contains two types of diluents: gradient dilution and sterile diluents.

Gradient dilution is the most widely used in the laboratory. It has phosphate-buffered saline, 1% buffered peptone water and 0.1% phosphate-buffered peptone saline. Other solutions can be customized according to customer needs. The wide push plate design of the front end of the gradient dilution liquid bottle cap minimizes the possibility of contact pollution when opening the cap; the tongue pressing design of the back end of the bottle cap facilitates the easy control of the opening angle of the cap by one hand, which make the operation more convenient, reasonable and convenient.

Sterile diluents mainly have MT-11 series, MV2-90 series, MV-225 series (wide mouth), MV2-1000 series and MV-1000 series, equipped with PBS, BPW, MRD and AP solutions. MT-11 series contains 9ml diluents, which is similar to gradient diluents GD-9 for gradient dilution. Customers can customize different types of diluents according to their own needs. The series of buffers are prepared with high standard substances under purified environment and sterilized by irradiation. They are suitable for dilution of different quantities of samples. Some products can be used in conjunction with various devices produced by our company to prevent secondary pollution, which is safe, highly efficient and easy to operate.

https://www.elexbio.com/diluents-series/

#diluent #diluents

Culture Media Series

In microbial detection, when the sample is collected and diluted correctly, the prepared sample solution needs to select the appropriate culture medium for cultivation, so as to judge whether the sample meets the standard. The choice of culture medium means whether we can get the desired experimental results quickly. ELEX is a culture media supplier providing culture medium that is mainly used for rapid detection, to make the microbial detection more simple and effective. The culture media of microbiology mainly include DNP Medi·Ca culture medium plate, concentrated liquid chromogenic medium, tri-color agar medium, and liquid chromogenic medium.

Different types of culture media for bacterial growth have their own characteristics. ELEX Biological, one of the most professional culture media suppliers, can provide different types of culture media with high quality. Compared with other agar media, DNP media is a kind of culture media for food microbiology, which makes the food microbial detection much easier, more standardized, more efficient and pollution-free. It can be used directly after opening, easy to see colony, and can reduce the cultivation area and waste gas. At present, AC test tablets are used to detect the total number of common colonies, CC test tablets to detect coliforms bacteria, EC test tablets to simultaneously detect E.coli and coliforms bacteria, SA test tablets to detect Staphylococcus aureus. All of ELMEX's Medi·Ca culture medium plate have passed the AOAC authoritative certification, and have been included in the Japanese food hygiene inspection guidelines.

Compared with the traditional medium, DNP Film·Media Culture Medium Plate has the following advantages. Firstly, the preparation process of the medium is omitted which saves time; the steps are reduced which lower the possibility of contamination in the process. Secondly, the medium plate can be placed overlapping, saving about 95% of the cultivation space, in addition to reducing emissions of exhaust gas and greenhouse gas (GHG), which save s environmental protection costs. Thirdly, it is easy to operate and does not depend on the operator's level. The bacterial colony is easy to see with convenient judgment and accurate result.

Compared with other similar products, it also has the following advantages: firstly, sample dropping can be carried out uniformly without pressure plate with fewer steps which is fast and convenient; secondly, there is a special anti-spillover ring, sample liquid will not spill and lead to cross-contamination.

Concentrated liquid chromogenic medium ST-SSP can be used in combination with our swab simpler, and can be used for the qualitative detection of E. coli and coliforms at the same time.

Tri-color agar medium is widely used for simultaneous qualitative and quantitative detection of coliform bacteria, Escherichia coli and other intestinal bacteria in food, water, milk, ice cream and meat products. Colonies show three colors, which can be easily judged and counted.

There are two kinds of chromogenic substances in liquid chromogenic medium, which can directly identify coliforms and Escherichia coli, but only for qualitative detection.

https://www.elexbio.com/culture-media-series/

#culture #media

Contact Agar Medium 4x4

Performance Characteristics of Contact Agar Medium 4x4

1. It is widely used in environmental sanitation, apparatus, sedimentation bacteria, and hand colony inspection.

2. It can be used directly after opening, no need to prepare a medium which saves much time.

3. The medium area is 10 cm². It's divided into 10 small cells which are easy to count.

Microbiology Agar Medium Types

AC • Standard Plate Counting Agar Based Medium

Used for the identification of the total bacteria number.

EC • Three-color Agar Based Medium

Escherichia coli and coliforms can be simultaneously detected.

E.coli colonies are blue to light purple.

Coliform colonies are red to purple.

Other intestinal bacterial colonies were milky white.

SA • Staphylococcus Aureus Agar Based Medium

Staphylococcus aureus forms black colonies on the surface of the culture medium, and due to protein breakdown, a transparent hemolysis ring or turbid zone is formed around the Staphylococcus aureus colonies.

PD • Yeast, Mold Agar Based Medium

Used for the identification of yeast and mold.

Usage Method of Contact Agar Medium 4x4

1. Remove the medium from the bag.

2. Open the lid and press the petri dish directly onto the sample to be sampled.

3. Cover the lid, mark it in the blank column of the container, and culture it in the incubator.

4. Colonies were counted after culture. Medium area: 10cm².

Product Details of Contact Agar Medium 4x4

Name

Characteristics

Package

Culture time

Validity Time

Agar Stamp AC

Total Bacteria Count Agar 10cm²

10pcs/bag; 100pcs/box

48±2 hours

6 months

Agar Stamp EC

Coliform group&E.coli Count Agar 10cm²

24 hours

Agar Stamp SA

Staphylococcus aureus Count Agar 10cm²

24~48 hours

Agar Stamp PD

Yeast&Mold Count Agar 10cm²

5 days

Note: 1. The product should be protected from light and stored at room temperature.

2. It should be used as soon as possible after unpacking.

3. When there are water droplets in the container, please gently remove the water droplets and use.

https://www.elexbio.com/contact-agar-medium-4x4.html

#agar #based

Contact Agar Medium 5x5

Performance Characteristics of Contact Agar Medium 5x5

1. It is widely used in environmental sanitation, apparatus, sedimentation bacteria and hand colony inspection.

2. It can be used directly after opening, no need to prepare medium which saves time and effort.

3. The medium area is 24 cm². It is divided into several small cells which are easy to count.

Agar Medium Type

AC• Standard Plate Counting Agar Medium

Used for the identification of the total bacteria number.

EC•Three-color agar medium

Escherichia coli and coliforms are simultaneously detected.

E.coli colonies are blue to light purple.

Coliform colonies are red to purple.

Other intestinal bacterial colonies were milky white.

SA•Staphylococcus aureus agar medium

Staphylococcus aureus forms black colonies on the surface of the culture medium, and due to protein breakdown, a transparent hemolysis ring or turbid zone is formed around the Staphylococcus aureus colonies.

PD•Yeast, mold agar medium

For the identification of yeast and mold.

Usage Method of Contact Agar Medium 5x5

1. Remove the medium from the bag.

Open the lid and press the petri dish directly onto the sample to be sampled.

3. Cover the lid, mark it in the blank column of the container, and culture it in the incubator.

4. Colonies were counted after culture. Medium area: 24 cm².

Product Details of Contact Agar Medium 5x5

Name

Characteristics

Package

Culture time

Validity Time

Agar Stamp AC

Total Bacteria Count Agar, 10cm²

10pcs/bag; 100pcs/box

48±2 hours

6 months

Agar Stamp EC

Coliform group&E.coli Count Agar, 10cm²

24 hours

Agar Stamp SA

Staphylococcus aureus Count Agar, 10cm²

24~48 hours

Agar Stamp PD

Yeast&Mold Count Agar, 10cm²

5 days

Note: 1. The product should be protected from light and stored at room temperature.

2. It should be used as soon as possible after unpacking.

3. When there are water droplets in the container, please gently remove the water droplets and use.

https://www.elexbio.com/contact-agar-medium.html

#5x5

Colony Counter

Smart iSac Simple Calculation 1. Simple Count Simultaneous calculation of 6 sheet medium; Correct the value of each medium; The result can be preserved as portrait data; The result cannot generate data documents; There are simultaneous counting functions of 6 sheet medium and a correction function for each medium value; Simply input an existing job.

Smart iSac Total Calculation 1. Simple count 2. OCR method Read 8 lines of numbers and automatically generates data documents. Automatically record bacterial numbers and image data The result can be output as a CSV file 8-line QR code for parallel use 3. Barcode method After the data is generated, the QR code can be used to generate the data document. Automatic recording of bacterial number and image data The result can be output as a CSV file

Equivalent media with other company Other company ELEX 3M TM culture media plate TM agar DNP Media·Ca culture media plate AC culture media plate for total bacteria number Media·Ca AC for total bacteria number RAC culture media plate for rapid measuring bacteria number / CC culture media plate for coliforms number Media·Ca CC for coliforms EC culture media plate for g E.coli and EC coliform Media·Ca EC for E.coli and coliforms STX culture media plate for S. aureus assay Media·Ca SA for Staphylococcus aureus

AQHC test piece for the identification of bacteria number in the water (subordinate vegetative bacteria)

Counting System Counting system simultaneous calculations of 6 sheet test piece Calculated value correction Data Preservation Original document Automatic document Colony conversion Qualified judgment CSV document Simple count ○ ○ ○※2 × × × × × OCR method ○ ○ ○ × ○ ○ ○ ○ Barcode method ○ ○ ○ ○ ○ ○ × ○ ※ 2 can be preserved as portrait data.

Specification of Automatic Bacterial Colony Counter Name Code No. Content Counting System Simple Count OCR method Barcode method Smart iSac simple calculation C001-5S Laptop ○ × × Scanner Smart iSac total calculation C001-5M Laptop ○ ○ ○ Scanner QR CODELEADER

Performance Characteristics of Automatic Bacterial Colony Counter 1. Rapid bacterial counting The processing capacity of simultaneous counting of 6 sheet culture media plate is about 6 times of the visual counting, which can further achieve the function of improving accuracy by the new technology; Culture media plate is used with DNP Media·Ca media, and 6 sheets can be read simultaneously by scanning. If the range and sensitivity of the count size need to modify, you can click on the above image and make the subtle adjustments by manual operation. Performance Characteristics 2. Data Preservation and Verification

Check data records, agar images, and calculated values, etc;

Preserve past data to reconfirm and compare;

The result can be output as a CSV file;

The result also can be converted to EXCEL and other files. 3. Massive Data Sharing

Set up a computer as server management or use a cloud server to achieve the headquarters' management of each factory.

https://www.elexbio.com/colony-counter.html

#colony #counter