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I do love when people make Calico Jack from Octonauts an actual Calico cat, because that implies that he's queer; since only female cats can be Calico with a few rare cases being intersex. So, most people imply Calico Jack is either a trans man, intersex, or both.
Y'know, it's an interesting little permutation that both Jack and Anne/Mary find it ridiculous/unbelievable/embarrassing that Ed and Stede have not actually had sex. Jack pushes Stede to admit whether they're "buggering each other" and Anne/Mary take their reticence to discuss their sex life as proof that they've not had sex. All three seem surprised or disgusted that Stede and Ed's relationship obviously does have an emotional component, yet not a physical one.
The lack of casual sex alongside the emotional connection angers and even threatens all three of the "regular" pirates (and Izzy - he is angrier at the thought of Ed crying over Stede than at the thought of Ed and Stede having sex).
Sexuality is permissible as long as it's emotionally unattached - and emotional attachment without sex is treated by the antagonists as embarrassing and ridiculous.
Oh hey, ever notice how the screen is divided evenly between them and they balance each other out in frame? And how Jack's appearance breaks that balance, coming between them and knocking them both off center?
Stede just looks so...deflated. Defeated. Tired. The dude went out looking for a new life as his old one was slowly killing him, and he's been told time and again that he's not enough: not manly enough, not pirate enough, not cool enough, not interesting enough, not adventurous enough. And it seems, even as he believes he knows Ed better than Jack does now, Ed will also think he's not enough. You can see the energy and life leaving him, because once again a bully has told him "you're not enough."