#contamfam

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[mycochaotix]

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Think before you spray, cut out, other wise fuck with sporulating molds

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Entire-HIghway-4070 submitted the above photos with the question: cubensis mycelium? and this additional comment added: "Literally blind..thinking it gets spread from having water moving on the surface? First time having bruising too with Agar. There's prob still some bacteria..T4s and T6 RW."

Mycochaos response:Okay, you have alot going on here and your comment with information is not fully understandable to me. That being said, here are my thoughts:- first picture, the mycelial colony on top left of the plate is a contaminant mould as it is gray compared to the mycelium coming from your previous agar xfer in center of plate) as well as it differs in morphology and spread of your xfered mycelium.- mycelium will bruise, the fruiting body of psilocybe fungi is a mushroom that is essentially a explosion of mycelium pushing together to where it forms a pin and then matures. The whole mushroom is mycelium and so both mycelium in substrate and mushroom skin (also mycelium) bruise blue. Bruising indicates psilocybin presence related to oxidation of that chemical, but also indicates damage to the mycelium... while bruising isnt "bad" in context of a break and shake, or rough handling of spawn... excessive bruising, especially over prolonged period where mycelium cannot "heal" "Reset" from, can damage the mycelium enough to cause its metabolic processes to cease.- why did you take transfers from inner parts of colonies. The recommended extraction location would be exterior growth, furthest away from suspected contaminants. This is primarily because as mycelium "ages" it grows outward, and so the exterior growth is most healthy and most virile, the inner mycelium at center of germination origin may start to vegetate and or slow down compared to exterior hyphae that are actively searching for nutrients.-Your xfer sizes seem large, you only need size of rice transfer (and this reduce chance of contaminants traveling with your transfer)--- So you would take a rice-size transfer from 2-3 o clock on this plate, the exterior edge of the white mycelium and maybe another from 6 o clock before you took out a huge chunk(?)- second picture, this one is harder to tell distinct colonies, and some of the mycelium visible is grayer and some white, but could be the camera lighting. again, xtract from exterior edges not inner bits from this plate until you get clean growth on future plates.- 3rd pic, not sure if this is same agar as pic 1 or 2, but from the angle in pic 3 i see mycelium in center, and then contaminant mold growing on outer edge and up the side facing the camera, and that growth to its right looks like similar contaminant growth. The growth over in the background of pic 3, to the top right of the main white mycelium growth, looks like mycelium growth maybe from the agar xfer rolling or some mycelium falling off it while you moved it to the center of plate prior to all this currently visible growthCheck these resources out for good info and examples of mycelium xfers and characteristics selection!Mycelium morphology : how to select healthy mcelium when breeding mushrooms -- https://www.youtube.com/watch?v=leUpfsonVxc&t=1smycotrophic - agar xfers/sectoring off healthiest growth - https://www.youtube.com/watch?v=XMxGwkj9Wn4bacterial colony morphology - https://www.youtube.com/watch?v=4JZAFUPckUgGood luck mycopal!DT Decontam and Sterilization Protocol: https://www.reddit.com/r/ContamFam/comments/jvvgjt/day_trippers_trip_tips_dts_decontamination_and/Contamfam Mycology dictionary: https://www.reddit.com/r/ContamFam/wiki/index/usefulpages/mycology_terminology/Recognizing and dealing with contamination: https://www.shroomery.org/forums/showflat.php/Number/23130868