Fun fact; I headcanon Giovanni as both bisexual and polyamorous. (I just scrolled through your Giovanni tag, oops)
Absolutely seconded! All the Auditores are queer. Every last one of them.
Giovanni: bi/pan and polyamorous as you said. He adores his wife, of course, but he loves his boyfriend Lorenzo too. (Maria knows about everything)
Maria: A-spec (possibly also bi/pan) absolutely fine with her husband being poly and actually benefits from it since she doesn’t want to have sex often.
Federico: see him as gay but he could be bi. Has a love/hate thing with Vieri. Whether he ever acted n it with Vieri is up for interpretation.
Claudia: a lesbian who freed herself from compulsory heterosexuality with the help of the brotherhood and her ownership of Rosa in Fiore
Petruccio: Gay. I know he was only 13 and may not have even known he liked boys yet but he was gay.
Mario: a gay man. He’s rich, pleasant, and fairly good looking but he never had a wife. He’s gay
Ezio: The pan-est pan ever to live. So pan he thinks everyone else is too. He’s not confused that Leonardo likes men but rather that Leonardo implied he didn’t also like women. Ezio is also polyamorous like his dad.