Mar 29, 2019
SDS-PAGE Hall of Shame
I found this hilarious website from Rice University and had to share!
The comments are so wonderful. I am currently laughing out loud at my desk xD
https://www.ruf.rice.edu/~bioslabs/studies/sds-page/sdsgoofs.html
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SDS-PAGE Hall of Shame
I found this hilarious website from Rice University and had to share!
The comments are so wonderful. I am currently laughing out loud at my desk xD
https://www.ruf.rice.edu/~bioslabs/studies/sds-page/sdsgoofs.html
So fucking happy to be done with this
I love staining protein gels or membranes. There’s something so fascinating about seeing proteins with your own eyes. Protein electrophoresis We separate proteins by size by loading protein mixtures onto a vertical gel. When electrical current is applied across the gel, proteins (which are negatively charged) will migrate along with the current, travelling through the gel. The gel contains tiny pores which allow proteins to move through it. Small proteins can move easily through these pores but large proteins will lag and move slower due to their size. Therefore, smaller proteins will travel further and end up towards the bottom of the gel, whereas larger proteins will travel a shorter distance and end up towards the top of the gel. The result is this band pattern, where proteins are arranged in decreasing size from the top of the gel to the bottom.
On the outer edges of the membrane (in blue), we’ve loaded a protein ladder. This is a commercial mixture of proteins which has been stained blue and contains multiple proteins of a range of known sizes. These are run and separated on a protein gel along with our protein mixtures so that we can compare the bands in our protein mixtures (stained in red) with the bands in the protein ladder (blue) and therefore calculate the size of our proteins.
March 2, 2017 >> Mixed, poured, set, loaded, ran, and visualized an SDS-PAGE gel all in one lab today 🔬 we loaded three samples from various previous labs to compare their relative amounts of target protein using SDS-PAGE visualization. I was very proud of our gel and babysat it through the entire 4 hour process.
The red palm weevil (Rhynchophorus ferrugineus) is one of the most destructive pests affecting economically important palm species worldwide. The present study investigated the insecticidal potential of the ethanol extract of Cocculus hirsutus and evaluated its effects on antioxidant enzyme activity and protein profiles in R. ferrugineus larvae. Leaves of C. hirsutus were collected and extracted using ethanol, and the chemical constituents of the extract were analysed through liquid chromatography–mass spectrometry (LC-MS). A total of 122 compounds were detected, including biologically active molecules such as hirsutine, gallic acid, resveratrol, ferulic acid, and quercetin derivatives. Larvae of R. ferrugineus were exposed to different concentrations (2–10%) of the plant extract. Higher concentrations (8% and 10%) caused rapid mortality of larvae, while the 2% treatment showed negligible effects and was excluded from subsequent biochemical analyses. Antioxidant enzyme assays revealed a marked reduction in catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), and polyphenol oxidase (PPO) activities in treated larvae compared with the control, indicating a disruption of oxidative stress defence mechanisms. SDS-PAGE analysis further demonstrated alterations in protein expression, with control larvae showing multiple protein bands, whereas treated larvae exhibited progressively faint bands at higher extract concentrations. These findings suggest that C. hirsutus ethanol extract induces oxidative stress and protein alterations in R. ferrugineus larvae, contributing to their mortality. The study highlights the potential of C. hirsutus as a promising botanical pesticide for the sustainable management of red palm weevil infestations.
The red palm weevil (Rhynchophorus ferrugineus) is one of the most destructive pests affecting economically important palm species worldwide. The present study investigated the insecticidal potential of the ethanol extract of Cocculus hirsutus and evaluated its effects on antioxidant enzyme activity and protein profiles in R. ferrugineus larvae. Leaves of C. hirsutus were collected and extracted using ethanol, and the chemical constituents of the extract were analysed through liquid chromatography–mass spectrometry (LC-MS). A total of 122 compounds were detected, including biologically active molecules such as hirsutine, gallic acid, resveratrol, ferulic acid, and quercetin derivatives. Larvae of R. ferrugineus were exposed to different concentrations (2–10%) of the plant extract. Higher concentrations (8% and 10%) caused rapid mortality of larvae, while the 2% treatment showed negligible effects and was excluded from subsequent biochemical analyses. Antioxidant enzyme assays revealed a marked reduction in catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), and polyphenol oxidase (PPO) activities in treated larvae compared with the control, indicating a disruption of oxidative stress defence mechanisms. SDS-PAGE analysis further demonstrated alterations in protein expression, with control larvae showing multiple protein bands, whereas treated larvae exhibited progressively faint bands at higher extract concentrations. These findings suggest that C. hirsutus ethanol extract induces oxidative stress and protein alterations in R. ferrugineus larvae, contributing to their mortality. The study highlights the potential of C. hirsutus as a promising botanical pesticide for the sustainable management of red palm weevil infestations.
The red palm weevil (Rhynchophorus ferrugineus) is one of the most destructive pests affecting economically important palm species worldwide. The present study investigated the insecticidal potential of the ethanol extract of Cocculus hirsutus and evaluated its effects on antioxidant enzyme activity and protein profiles in R. ferrugineus larvae. Leaves of C. hirsutus were collected and extracted using ethanol, and the chemical constituents of the extract were analysed through liquid chromatography–mass spectrometry (LC-MS). A total of 122 compounds were detected, including biologically active molecules such as hirsutine, gallic acid, resveratrol, ferulic acid, and quercetin derivatives. Larvae of R. ferrugineus were exposed to different concentrations (2–10%) of the plant extract. Higher concentrations (8% and 10%) caused rapid mortality of larvae, while the 2% treatment showed negligible effects and was excluded from subsequent biochemical analyses. Antioxidant enzyme assays revealed a marked reduction in catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), and polyphenol oxidase (PPO) activities in treated larvae compared with the control, indicating a disruption of oxidative stress defence mechanisms. SDS-PAGE analysis further demonstrated alterations in protein expression, with control larvae showing multiple protein bands, whereas treated larvae exhibited progressively faint bands at higher extract concentrations. These findings suggest that C. hirsutus ethanol extract induces oxidative stress and protein alterations in R. ferrugineus larvae, contributing to their mortality. The study highlights the potential of C. hirsutus as a promising botanical pesticide for the sustainable management of red palm weevil infestations.
watching this run for 1 hour made me very hungry...
eat it
eat it
no don't eat it it's the first time this assay has worked so well!!!!!!!!!