#wistar rat

[lechemugs]

I've launched the first chapter of my new webcomic, "ScampStreet!" It's available on WebToon, but I will be posting chapters here aswell!

Comic under the cut! Enjoy!

[oaresearchpaper]

There is increasing evidence in support of a decisive role played by the diet in the development of non-communicable diseases. The present study aimed to assess the effect of salt overfeeding on renal function and on the cardiovascular system in Wistar rats. Four groups of rats were exposed to diets with various salt levels, 0.8% for control, and 2%, 4% and 8% for overfed rats during 12 weeks. Blood Glucose, Triglycerides, Total cholesterol, LDL cholesterol (LDL-c) and HDL cholesterol (HDL-c) levels were determined by enzymatic method and serum PCSK9 by ELISA. Kidneys’ histology sections were treated with hematoxylin-eosin staining. Serum creatinine (17.54±2.35 mg/L vs. 11.06±0.95 mg/L; p

[oaresearchpaper]

Cadmium is a persistent environmental contaminant that induces oxidative stress and disrupts renal function, posing serious risks to human and animal health. This study evaluated the protective effects of okra seed oil against cadmium-induced nephrotoxicity in male Wistar rats. The rats were divided into four groups: a healthy control group, a cadmium-exposed group, a group receiving okra seed oil alone, and a co-administrated cadmium and okra seed oil group. Biochemical analysis revealed that cadmium exposure significantly impaired renal function, disrupted protein and glucose regulation, and increased oxidative stress markers, indicating severe metabolic and functional disturbances. In contrast, rats treated with okra seed oil alone maintained normal biochemical profiles, demonstrating the safety of the oil. The co-administration of okra seed oil with cadmium significantly mitigated these negative effects, restoring several biochemical parameters toward control levels. Histopathological examination supported these findings. Kidneys from cadmium-exposed rats exhibited glomerular shrinkage, widening of Bowman’s spaces, tubular degeneration, medullary disorganization, and congestion. Conversely, kidneys from rats treated with okra seed oil alone maintained their normal architecture. Notably, co-administration of okra seed oil with cadmium markedly reduced these lesions, with partial preservation of glomerular and tubular morphology, improved cortical organization, and decreased medullary congestion. Overall, these results indicate that okra seed oil provides both functional and structural protection against cadmium-induced renal injury. Its antioxidant and anti-inflammatory properties serves as important biochemical and histological markers, highlighting okra seed oil as a promising natural intervention for heavy metal-induced nephrotoxicity.

[bookpiofficial]

Kerosene: A Study of Tissue Histology and Serum Vitamin and Heavy Metal Levels of Female Wistar Rats Chronically Exposed | Chapter 07 | Advances and Trends in Biotechnology and Genetics Vol. 2

Background: Kerosene a commonly available product used for a variety of purposes in many parts of Asia and Africa is sometimes sold in beverage bottles and jerry cans in both commercial and residential places due to inadequate number of filling stations. Therefore excessive exposure through both dermal and oral routes is common.

Objective: This study was embarked upon to ascertain the impact of trace amount of kerosene on tissue histology as well as serum vitamin and heavy metal levels in female Wistar rats.

Methods: Kerosene (0.4 mL/kg body weight) was administered to rats either through the oral or dermal route daily for a period of 30 days. The effects of kerosene administration on tissue histology; serum vitamin levels and serum concentrations of heavy metals were evaluated using hematoxylin- eosin staining technique; high performance liquid chromatography (HPLC); and atomic absorption spectrometry (AAS) respectively. Student’s t test and analysis of variance were used for statistical analysis of data. P <0.05 was considered significant.

Results: Histopathologic presentation such as pulmonary congestion, severely stunted villi, congestion of coronary vessels, and diffuse spongiosis of the cerebral cortex were observed in kerosene administered groups while control group featured no visible lesion. Results of heavy metals revealed that although As, Al and Cd were not significantly different in kerosene exposed groups compared with control, Si was significantly lower (oral), and significantly higer (dermal) compared with control. In addition, using analysis of variance (ANOVA) all estimated vitamins (except pantothenic acid) were significantly different in kerosene exposed groups compared with control.  

Conclusion: The results of this study indicate that exposure to this product either through the oral or dermal route may be detrimental to health as it induced adverse alteration in vitamin and heavy metal levels as well as distorted tissue histo-architecture.

Author(s) Details

Ayobola A. Iyanda Department of Chemical Pathology, College of Health Sciences, Ladoke Akintola University of Technology, Osogbo, Nigeria.

Read full article: http://bp.bookpi.org/index.php/bpi/catalog/view/82/1168/811-1

View Volume: https://doi.org/10.9734/bpi/atbg/v2

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