#zc45

[didanawisgi]

SARS coronavirus (SARS-CoV), the causative agent of the large SARS outbreak in 2003, originated in bats. Many SARS-like coronaviruses (SL-CoVs) have been detected in bats, particularly those that reside in China, Europe, and Africa. To further understand ...

Abstract

SARS coronavirus (SARS-CoV), the causative agent of the large SARS outbreak in 2003, originated in bats. Many SARS-like coronaviruses (SL-CoVs) have been detected in bats, particularly those that reside in China, Europe, and Africa. To further understand the evolutionary relationship between SARS-CoV and its reservoirs, 334 bats were collected from Zhoushan city, Zhejiang province, China, between 2015 and 2017. PCR amplification of the conserved coronaviral protein RdRp detected coronaviruses in 26.65% of bats belonging to this region, and this number was influenced by seasonal changes. Full genomic analyses of the two new SL-CoVs from Zhoushan (ZXC21 and ZC45) showed that their genomes were 29,732 nucleotides (nt) and 29,802 nt in length, respectively, with 13 open reading frames (ORFs). These results revealed 81% shared nucleotide identity with human/civet SARS CoVs, which was more distant than that observed previously for bat SL-CoVs in China. Importantly, using pathogenic tests, we found that the virus can reproduce and cause disease in suckling rats, and further studies showed that the virus-like particles can be observed in the brains of suckling rats by electron microscopy. Thus, this study increased our understanding of the genetic diversity of the SL-CoVs carried by bats and also provided a new perspective to study the possibility of cross-species transmission of SL-CoVs using suckling rats as an animal model.

Emerg Microbes Infect. 2018; 7: 154. Published online 2018 Sep 12. doi: 10.1038/s41426-018-0155-5

[kramlabs]

Ordered both

Inconsistent with Evolution Mug - Biosafety Now Shop

Inconsistent with Evolution Mug! High quality 15oz white gloss ceramic mug Decorated with full wrap dye sublimation Microwave & Dishwash

[kramlabs]

#1: A 'consensus' sequence of SARS like events

Leading up to patient 0 in Wuhan

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Divergent SARS-CoV-2 variant emerges in white-tailed deer with deer-to-human transmission - Nature Microbiology

White-tailed deer are a potential reservoir of SARS-CoV-2 variants.

Montana Mice May Hold the Secret to Virus Spillover

Researchers in Montana are working to figure out how climate change and biodiversity affect viruses’ jump from animals to people.

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SARS-CoV-2 infection, neuropathogenesis and transmission among deer mice: Implications for reverse zoonosis to New World rodents

Coronavirus disease-19 (COVID-19) emerged in November, 2019 in China and rapidly became pandemic. As with other coronaviruses, a preponderan

[kramlabs]

The Ralph Baric smoking gun?

“SARS-CoV-2 has a telltale signature of genetic engineering, not previously identified . That genetic fingerprint suggests the work of Ralph Baric, Ph.D., was used in the creation of the virus. There’s a direct match between Baric’s published research — which describes how to hide telltale signs of genetic engineering — and the genetics found in SARS-CoV-2

In 2002, Baric invented a technique called seamless ligation, which conceals all evidence of genetic engineering in lab-created pathogens. Baric’s nickname for this technique is the “no-see’m method.” Baric taught the method to Shi Zhengli in 2016, and Shi and her colleagues at the Wuhan Institute of Virology (WIV) demonstrated mastery of Baric’s technique in a series of gain-of-function experiments. However, while seamless ligation conceals human tampering in lab-created pathogens, the method leaves a signature of its own, and that’s the signature discovered in SARS-CoV-2.

The findings raise the possibility of liability for the University of North Carolina where Baric works, the National Institutes of Allergy and Infectious Diseases, which funded Baric, the WIV and other parties”

H/T

Systematic Assembly of a Full-Length Infectious cDNA of Mouse Hepatitis Virus Strain A59 | Journal of Virology

ABSTRACT A novel method was developed to assemble a full-length infectious cDNA of the group II coronavirus mouse hepatitis virus strain A5

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April 2020:

Baric. Google Ralph Baric.

- https://www.youtube.com/watch?v=LlplnH3VYyc -

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A SARS-like cluster of circulating bat coronaviruses shows potential for human emergence - Nature Medicine

Ralph Baric, Vineet Menachery and colleagues characterize a SARS-like coronavirus circulating in Chinese horseshoe bats to determine its pot

[kramlabs]

Reminder:

Researchers say one in 3 trillion chance Covid developed the code naturally

Scientists find genetic match between Covid and Moderna 2016 patent

The international team of researchers identified a tiny snippet of code that is identical to part of a genetic sequence patented by the US

https://www.frontiersin.org/articles/10.3389/fviro.2022.834808/full#B10

MSH3 Homology and Potential Recombination Link to SARS-CoV-2 Furin Cleavage Site

Among numerous point mutation differences between the SARS-CoV-2 and the bat RaTG13 coronavirus, only the 12-nucleotide furin cleavage site

https://www.ncbi.nlm.nih.gov/labs/pmc/articles/PMC7457603/

Furin Cleavage Site Is Key to SARS-CoV-2 Pathogenesis

SARS-CoV-2 has resulted in a global pandemic and shutdown economies around the world. Sequence analysis indicates that the novel coronavirus

[didanawisgi]

SARS coronavirus (SARS-CoV), the causative agent of the large SARS outbreak in 2003, originated in bats. Many SARS-like coronaviruses (SL-CoVs) have been detected in bats, particularly those that reside in China, Europe, and Africa. To further understand ...

Abstract

SARS coronavirus (SARS-CoV), the causative agent of the large SARS outbreak in 2003, originated in bats. Many SARS-like coronaviruses (SL-CoVs) have been detected in bats, particularly those that reside in China, Europe, and Africa. To further understand the evolutionary relationship between SARS-CoV and its reservoirs, 334 bats were collected from Zhoushan city, Zhejiang province, China, between 2015 and 2017. PCR amplification of the conserved coronaviral protein RdRp detected coronaviruses in 26.65% of bats belonging to this region, and this number was influenced by seasonal changes. Full genomic analyses of the two new SL-CoVs from Zhoushan (ZXC21 and ZC45) showed that their genomes were 29,732 nucleotides (nt) and 29,802 nt in length, respectively, with 13 open reading frames (ORFs). These results revealed 81% shared nucleotide identity with human/civet SARS CoVs, which was more distant than that observed previously for bat SL-CoVs in China. Importantly, using pathogenic tests, we found that the virus can reproduce and cause disease in suckling rats, and further studies showed that the virus-like particles can be observed in the brains of suckling rats by electron microscopy. Thus, this study increased our understanding of the genetic diversity of the SL-CoVs carried by bats and also provided a new perspective to study the possibility of cross-species transmission of SL-CoVs using suckling rats as an animal model.

1Department of Epidemiology, College of Preventive Medicine, Third Military Medical University, Chongqing, 400038 China

2Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, 210002 China

[kramlabs]

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Luigi Warren SARS-CoV-2 thesis

Thread from twitter: https://twitter.com/luigi_warren/status/1259826618442235909 H/t JC on a bike Severe acute respiratory syndrome (SARS) i

https://www.wanderwoodsandwater.com/post/622525181518282752/cloaked-similarity-between-hiv-1-and-sars-cov

[kramlabs]

‘We Made a Big Mistake’ — COVID Vaccine Spike Protein Travels From Injection Site, Can Cause Organ Damage • Children's Health Defense

Research obtained by a group of scientists shows the COVID vaccine spike protein can travel from the injection site and accumulate in organs