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for differential identification of Gram-negative enteric bacilli on the basis of the fermentation of dextrose, lactose and H2S Production (Store below 8° C)

Kligler Iron Agar for differential identification of Gram-negative enteric bacilli on the basis of the fermentation of dextrose, lactose and H2S Production (Store below 8° C)

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Microbial contamination of pharmaceuticals has been posing severe challenges for the researchers pharmaceutical manufacturers throughout the generations. They lead to greater problems both medically and economically. Contaminations can result in the batch failures as the pathogens breakdown…

for standard test of lactose fermenting coliforms.

The selectivity of Endo Agar is due to Sodium sulfite / Basic fuchsin combination, which results in the suppression of gram positive organisms. Coliforms ferment the lactose, produce pink to rose red colonies and similar colouration of the medium.

for identification of bacteria on the basis of urea utilization, especially for the differentiation of Proteus, Salmonella and Shigella species.

UREA BROTH is used for the identification of bacteria on the basis of urea utilization, especially for the differentiation of Proteus, Salmonella and Shigella species. Medium contains limited nutrient and a high buffering capacity. These characteristics make the medium particularly suited for the differentiation of Proteus species from other gram negative enteric bacilli capable of utilizing urea but unable to do so in the UREA BROTH.

Lactophenol cotton blue is a mixture of methyl blue, a histological stain, and lactophenol. It is used in wet-mount preparations for visualization of fungal structures, especially in medical mycology.

for detection of starch hydrolysing microorganisms.

Flood the surface of 48 hours old culture on Starch Agar with Grams Iodine (TBL 034). Starch hydrolysis is seen as a colourless zone surrounding the colonies. A blue or purple zone indicates that starch is not hydrolyzed. Size of the clear zone is directly proportional to the starch hydrolyzing activity of the strain under study.

TM Media offers an extensive variety of Dehydrated Culture Media, keeping in mind the end goal to create the best outcomes in any microbiological studies. These dehydrated culture media solely for these purposes including various forms of Agar and Broth, with such a wide selection both vegetable and animal origin culture media.

Simmons Citrate Agar for differentiation of Enterobacteriaceae on the basis of citrate utilization

Simmons Citrate Agar uses citric acid as the carbon source. It is used to differentiate Salmonella groups from each other. It is useful for selecting for organisms that use citrate as its main carbon and energy source.

Triple Sugar Iron Agar is used (TSI Agar)for confirmation of gram negative enteric bacilli on basis of dextrose, lactose and sucrose fermentation and H2S production or confirmation of gram negative enteric bacilli on basis of dextrose, lactose and sucrose fermentation and H2S production. It is recommended by BIS committee under specification IS:5887 (Part I, III and V) 1976,

Hektoen Enteric Agar was developed in 1967 by King and Metzger of the Hektoen Institute in order to increase the frequencies of isolation of Shigella and Salmonella organisms when compared with their recovery on other media frequently utilized in clinical laboratories at that time. Sodium…

Hektoen Enteric Agar as part of the multi-step procedure to isolate Salmonella. This medium is recommended by United States Pharmacopoeia, 2009 for testing the presence of Salmonella in dietary supplements. This medium is recommended in testing of Salmonella in food sample by various standards.

There are several companies in the market that offer a wide array of Microbiology and Biotechnology Products. All these products have gained a wide appreciation in the food beverage, pharmaceutical, and water testing industries. These chemical products are mainly required for developing…

There are a number of companies in the market that are engaged in providing a wide range of microbiology products. The range is highly preferred by the clients as it helps in managing live organisms in a rightful manner. Some of the products include in the range of microbiology products are blood agar base, brain heart infusion broth, buffered pep-tone water and cetrimide agar base, so on. All these products are readily available in the market at highly reasonable prices.

Types of Microbiology and Biotechnology Products

There are several companies in the market that offer a wide array of Microbiology and Biotechnology Products. All these products have gained a wide appreciation in the food & beverage, pharmaceutical, and water testing industries. These chemical products are mainly required for developing recognition and maintenance of a particular group of micro-organisms. The development of microorganisms in particular media assists to determine and treat the cause of the problem leading to effective treatment of assorted infections. Here are some of the microbiology and biotechnology products available in the market:

Biological Products

Biological products can be combined of carbohydrates, proteins, or nucleic acids or composite collections of these components, or may be living substances like cells and tissues. These products are separated from a number of organic sources - human, animal, or organism - and can be generated by biotechnology procedures and other methods. Gene-based and cellular biological products, as an instance, often are at the head of the main experimentation, and may be preferred to deal with a number of medical statuses for which no other methods are accessible.

Microbiology Products

There are a number of companies in the market that are engaged in providing a wide range of microbiology products. The range is highly preferred by the clients as it helps in managing live organisms in a rightful manner. Some of the products include in the range of microbiology products are blood agar base, brain heart infusion broth, buffered pep-tone water and cetrimide agar base, so on. All these products are readily available in the market at highly reasonable prices.

Blood Agar Base: Blood Agar Base (Infusion Agar) is used for isolation, cultivation of fastidious pathogenic microorganisms after addition of blood. Blood Agar Base media can be used as general-purpose media also, without blood addition. Blood agar base medium can also be used for primary isolation of Haemophilusspecies, where defibrinated sheep blood is used to enrich the medium. Blood Agar Base (Infusion Agar) contains a rich nutrient base, which provides optimal growth conditions for all relevant microorganisms. Tryptone and Brain Heartinfusion supply amino acids, minerals and other essential growth factors in the medium for the growth of microorganisms. Sodium chloride maintains the osmotic balance of the medium. Agar is a solidifying agent. On addition of blood, the pH value of 6.8 stabilizes and favors the formation of clear hemolysis zones. Fresh, defibrinated sheep blood is most suitable for determent hemolysis forms. It gives best results for Group A Streptococci. But sheep blood fails to support the growth of Haemophilus haemolyticus due to deficiency of pyridine nucleotides. However, the addition of horse blood supports H. haemolyticus growth and hemolysis and the growing colonies mimic Streptococcus pyogenes.

Brain Heart Infusion Broth: Brain Heart Infusion Broth (BHI Medium) for cultivation of fastidious microorganisms associated with blood culture. Brain Heart Infusion Broth (BHI Medium) is useful for cultivating a wide variety of microorganisms since it is a highly nutritive medium. This medium is recommended by Food and Drug Administration. This culture medium complies with the specifications given by EN ISO 6888 and APHA. It is also used to prepare the inocula for antimicrobial susceptibility testing. BHI Broth is a modification of the original formulation of Rosenow, where he added pieces of brain tissues to dextrose broth. BHI Broth is also the preferred medium for anaerobic bacteria, yeasts and moulds. Brain Heart Infusion Broth (BHI Medium) is nutritious and well buffered to support the growth of wide variety of organisms. With the addition of 10% defibrinated sheep blood, it is useful for isolation and cultivation of Histoplasma capsulatum and other fungi. For selective isolation of fungi, addition of gentamicin and/or chloramphenicol is recommended. This medium is not suitable for obtaining characteristic haemolytic reactions even after addition of blood because of its glucose content.

SIMMONS CITRATE AGAR: Simmons citrate Agar is used for differentiation of gram-negative bacteria on the basis of citrate utilization. Citrate acts source of carbon and nitrogen in the medium. Ammonium dihydrogen phosphate provides nitrogen in the medium. Dipotassium phosphate acts as a buffer. Sodium chloride maintains the osmotic balance of the medium. Sodium citrate is the sole source of carbon in this medium. Magnesium sulphate is a cofactor for the metabolic process. Organisms that can utilize Ammonium dihydrogen phosphate and Sodium citrate as their sole sources of nitrogen and carbon will grow on this medium and produce a color change from green (neutral) to blue (alkaline). Agar is the solidifying agent. The alkaline reaction takes place when excess CO2 is generated (Kreb’s cycle) as citrate is cleaved to form oxaloacetate. Oxaloacetate is decarboxylated to pyruvic acid and CO2. The excess CO2 combines with sodium and water that is already present in the medium to form sodium carbonate. In addition, bacteria that utilize citrate can extract nitrogen from the ammonium phosphate present in the medium, forming ammonia which combines with water to form NH4OH. The formation of these end products produce an alkaline pH in the medium (greater than 7.6), resulting in a color change which is detected by Bromothymol blue. Colour change from green to blue is the indication of positive reaction.

TM Media offers an extensive variety of Dehydrated Culture Media, keeping in mind the end goal to create the best outcomes in any microbiological studies. These dehydrated culture media solely for these purposes including various forms of Agar and Broth, with such a wide selection both vegetable and animal origin culture media. All these are broadly used in Microbiology, Research Work, Pharma, Veterinary, Waste Water, Food and Dairy Industries, etc.

Bile Esculin Agar containing 4% bile salts was formulated by Swan and modified by Facklam and Moody. Bile Esculin Agar is also recommended by APHA for identification of Group D Streptococci. Peptic digest of animal tissue and beef extract serve as source of carbon, nitrogen and essential growth factors. Enterococci and certain streptococci hydrolyze the glycoside esculin to esculetin and dextrose.

CETRIMIDE AGAR is used for the selective isolation of Pseudomonas aeruginosa from clinical samples. The medium is a modification of the medium proposed by Brown and Lowbury (1965) for the isolation and differentiation of Pseudomonas aeruginosa from various materials

Sabouraud Dextrose Agar or SDA contains Mycological peptone provide nitrogen and vitamin source required for organism growth. Due to its low pH this medium is very sensitive to overheating which will soften the agar and caramelize the carbohydrate. Agar is the solidifying agent.

For selective isolation of fastidious pathogenic microorganisms after addition of blood.

TM Media is a leading brand for microbiology products for laboratory, pharmaceutical, hospital and many more industries. They have huge range of products on different categories.

Blood Agar Base (Infusion Agar) is used for isolation, cultivation of fastidious pathogenic microorganisms after addition of blood. Blood Agar Base media can be used as general-purpose media also, without blood addition. Blood agar base medium can also be used for primary isolation of Haemophilusspecies, where defibrinated sheep blood is used to enrich the medium.

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