yo′ Echizen

Won't you notice me? ♥ BS in Biology | 20 | FEU

[yoechizen]

Somehow, I am still stuck after trying so, so hard... Maybe I didn't? Maybe I did. And did not.

I don't need saving. I can't be saved. I need to be understood.

-2:43

[yoechizen]

I just wanted to change

I just wanted a change

But here I am

Still stuck

I am still stuck...

I am still a terrible person

Up to no good.

-2:38

[yoechizen]

Why would you wish upon a star?

It is dead.

Pray instead.

070818

[yoechizen]

062918

Pizza huuuut

Nung umaga, hinayaan lang kaming gumawa ng thesis sa lab kasi busy din sila.

Nung tanghali naman, nagcheck ulit kami ng results ng 🦀 biochemical test.

Yung methyl red test for e. coli at salmonella: distinct red = positive.

Then sa plate naman na may colonies sa emb at hea, xld, bsa. Kumuha kami ng isolated colony tapos inistreak sa slant.

Bsa, hea, xld for lia at tsia. Lia = violet Tsia = red. Streak sa lia then stab then double stab sa tsia then streak.

Then sa emb, naghanap lang kami ng dilution na may iso colony tapos yun lang yung inistreak namin sa nutrient agar.

[yoechizen]

Hirap sating mga Pinoy ginagawang buffet yung defense e. Kingina 2k daw para sa food ng mga panel. Ano na FEU?

[yoechizen]

062818

Ojt: Wala kami masyadong ginawa kanina. Nagcheck lang ng result sa biochem test ( Brilliant Green Bile Broth for fecal count and EC for total coliform {both may effervescent or bubble sa Durham's tube} [E. coli] ) Yung EC naman inistreak namin sa EMB (dark brown na kami rin yung nagprepare \:D/

Rappaport V. {Dark blue positive:faded blue} for Salmonella ay inistreak naman namin HEA, Xylose lysine d., at Bismuth sulfa(i)te agar. to isolate black colonies.

Then naglipat din kami ng Salmonella at E. coli na galing sa slant sa emb.

Then naghugas na kami for one hour ng mga ginamit na tubes.

[yoechizen]

062518

Nakapencil skirt

Nalate

Nagquiz kami 14/15 ako

Nagcheck ng result

[yoechizen]

062718

8:22 dumating

May dumating na tao para magcalibrate ng pcr

Nagprepare ako ng emb:

350 mL distilled = 350 mL sample × 37.5g ÷ 1000L then pour plate stay sa ref

Naglipat ng salmonella at e. Coli control week 3 day 1

May +10 ojt daw for 1 week from visayas

Naglipat ng sample sa rv (blue) at ec (yellowish) at bgbb (green) tapos water bath

Nagcheck ng result sa ymc (pink) [velvety = mold] [10 to 150 valid count] at

biochemical test:

Barret a + b stand 2hrs red = positive

Kumain sa Jollibee

[yoechizen]

062618

Bumili ng tinapay sa bakery.

Nakalimutan payong, salamin, tsinelas

Nagdissect ng tilapia (black chin)

Test ulit for e. Coli at salmonella

Record yung mga nakaraang test

Kumain ng pizza

[yoechizen]

If you don't want to take a risk

Then you are not worth the risk.

[yoechizen]

The fault in our star is that, we always wanted everyone to remember us. We are afraid to be forgotten.

The second fault is that, we don't really need everyone. We just need that only one, each night, when the darkness fell, to come look up on us and smile.

[yoechizen]

062318

Half day lang kami ngayon.

Plates:

EMB = Brown (for fecal count) kumuha kami kanina sa plates namin then nilipat sa PCA slant.

XLD = Orange (isolation for E. coli at Salmonella same with HEA at BSA)

HEA = Dark Green

BSA = Matcha

Broth: Kuha sa plate gamit yung butt ng inoculating loop then streak sa TSIA at stab. Then stab 2x yung LIA saka streak. No need to change loop if same plate.

TSIA = Red

BGBB: Brilliant Green Bile Broth (tuloy-tuloy na 48 hrs incubation). Total coliform. Bubbles in durham tube or

LIA = Violet

Then may quiz sa Monday.

Liquids:

Brilliant Green Bile Broth with Durham Tube for the Detection of total coliform. Positive = bubble in durham or effervescent.

Last, pinakita samin ni mam yung result ng yeast and mold na ginawa ni Jason at sir nung Monday.

[yoechizen]

Nasa punto na ako ng buhay ko na wala ng punto yung buhay ko :D

[yoechizen]

Ako yung tipo ng mag-aaral na the more na maganda yung sulat ko, the more na walang pumasok sa isip ko.

[yoechizen]

[yoechizen]

062018

Dumating ako sa site nang 8:07

Una, nagweigh muna kami ng 25g crabs' meat. Ayos naman, smooth.

Then dilution na medyo mahirap (?) nang very very slight dahil need ko iipit yung small cap ng test tube sa hinliliit ko.

Then streak lang ng E. coli

Then uwi na.

Pero hindi na ako sumakay sa bus. Doon ako sumakay sa Q-Mart ng Crame papasok.

[yoechizen]

061918

Late ako kanina sa biores dahil ang tagal ko gumalaw. Hindi na dapat ako papasok.

Naligaw kami konti papunta sa site.

Nalito ako sa tinimbang ko kung pca ba yun o ltb.

Muntikan nanaman ako lumagpas sa Crame.

Sa papasok na lang ako sumakay kasi walang adamson.