All Things Chemistry @chemistrybug-blog - Tumblr Blog

High-performance liquid chromatography- (sometimes referred to as high-pressure liquid chromatography), HPLC, is a chromatographic technique used to separate a mixture of compounds in analytical chemistry and biochemistry with the purpose of identifying, quantifying and purifying the individual components of the mixture.

#High-performance liquid chromatography (sometimes referred to as high-pressure liquid chromatography) HPLC is a chromatographic technique...#high #performance #youtube #hplc #chemistry #lover #chembug #213 #rob #craig #csi #cuny #smart stuff

Project 3: 2D (NOESY) spectra FT-NMR of Proteins

In this project, students will record and analyze the 2D spectrum with two frequency axes of proteins.

Data will be acquired at the end of the experiment (detection, often called direct evolution time); and during this time labelled with the chemical shift of the second nucleus. Mixing sequences utilize two mechanisms for magnetization transfer: scalar coupling or dipolar interaction (NOE)

Students will become familiar with 2D spectrums

Two dimensional FT yields the 2D spectrum with two frequency axes. If the spectrum is homonuclear (signals of the same isotope (usually 1H) are detected during the two evolution periods) it has a characteristic topology:

The NOESY experiment is crucial for the determination of protein structure. It uses the dipolar interaction of spins (the nuclear Overhauser effect, NOE) for correlation of protons. The intensity of the NOE is in first approximation propotional to 1/r6, with r being the distance between the protons: The correlation between two protons depends on the distance between them, but normally a signal is only observed if their distance is smaller than 5 Å. The NOESY experiment correlates all protons which are close enough. It also correlates protons which are distant in the amino acid sequence but close in space due to tertiary structure. This is the most important information for the determination of protein structures.

#Project 3: 2D (NOESY) spectra FT-NMR of Proteins.#In this project students will record and analyze the 2D spectrum with two frequency axes of proteins. Data will be acquired at the end o...#chem lover for life #chemistry #chem #c #chemistryyy

Project 2: Quantum mechanical calculations using Gaussian “09 software for the energy conformations of Aspirin

Gaussian 09

Gaussian 09 is the latest in the Gaussian series of programs. It provides state-of-the-art capabilities for electronic structure modeling. Gaussian 09 contains every scientific/modeling feature, and none imposes any artificial limitations on calculations other than your computing resource. This software platform will be used to investigate Aspirin and other small molecules, by the students in the department of chemistry at the College of Staten Island.

#Project 2: Quantum mechanical calculations using Gaussian “09 software for the energy conformations of Aspirin #Gaussian 09 Gaussian 09 is the latest in the Gaussian series of programs. It provides state-of-the-art capabilities for electronic struct...#gaussian 09 #chem lover #chemistry #Chem Lecture

Project 1: FT -NMR of Aspirin

Proton Nuclear Magnetic Resonance (1H NMR) Spectroscopy is a powerful method used in the determination of the structure of unknown organic compounds

Students will be using NMR extensively to help assign structures to organic compounds, namely aspirin and other medicinal compounds.

The 1H NMR spectrum of an organic compound provides information concerning:

the # of different types of hydrogens present in the molecule

the relative #'s of the different types of hydrogens

the electronic environment of the different types of hydrogens

the number of hydrogen "neighbor" a hydrogen has