Myogenesis
[how mature muscle cells are made]
Muscle fibers generally form the fusion of myoblasts into multi-nucleated fibers called myotubes.
In early development, myoblasts can either proliferate, or differentiate into a myotube.
once differentiated, myoblasts align
cells fuse - presence of calcium ions is critical, involves recruitment of actin to the plasma membrane, followed by close apposition and creation of a pore that subsequently rapidly widens.
Stages and associated genetic factors
Delamination --> PAX3, c-Met
Migration --> c-met/HGF, LBX1
Proliferation --> PAX3, c-Met, Mox2, MSX1, Six1/4, Myf5, MyoD
Determination -->Myf5 and MyoD
Differentiation --> Myogenin, MCF2, Six1/4, MyoD, Myf6
Specific Muscle Formation --> Lbx1, Meox2
Satellite Cells --> PAX7
