#paenibacillus

[theyuanjourneys]

The First Activity of My Apprenticeship - Explore Microbe and It's Application

Hi everyone!!! This is the first post and i'll start updating what i learned or i got from apprenticeship session. Also i don't know what do you guys prefer for language that i used, should i use Bahasa Indonesia or stay in English Language.

Those pictures were me and the crew making Paenibacillus polymyxa culture, a bacteria species act as Biological Control Agent. Paenibacillus polymyxa could secrete protease, amylase, β-glucanase, and cellulase which surprisingly inhibiting fungal diseases as antagonistic substances by disrupting the cell wall structure. Antagonistic mechanism of P. polymyxa will also form biofilm which help these bacteria becoming dominant colony. The production of siderophore and indole-3-acetic acid help plant that bioinoculated by these bacteria thrive more nicely (Yang et al., 2024).

Paenibacillus polymyxa can be cultured in-vitro using Nutrient Broth/Agar or Tryptone Soy Broth/Agar. But! If we want to use this for application in agriculture that will be expensive due to the media. We actually overcome this by making Soy Extract obtained from residential industry of soy tofu. Since filthy soy milk was a waste, we mixed with water and sugar to form medium culture with the specific ratio.

For the ingredients are:

20 liters of filthy soy milk

600 grams of sugar

4 slant isolate test tube of Paenibacillus polymyxa

All the ingredients were pasteurized at boiling point, except we don't to pasteurized Paenibacillus polymyxa, otherwise the bacteria died. LMFAO. After it has been inoculated, it must be controlled by growing in the aerobic environment, since Paenibacillus polymyxa a facultative anaerobe microorganism (Meena et al., 2017).

We make that by creating system with clean air injected using Glass wool, KMnO4 as oxidation agent or simply kill any microbe in the air. The sterilized air than enter the jerry can.

If already done fermented in 2 weeks, we can actually obtain and package in 5 liters jerry cans. These activities are new to me and makes me excited :>

References

Meena, V. S., Mishra, P. K., Bisht, J. K., & Pattanayak, A. (Eds.). (2017). Agriculturally important microbes for sustainable agriculture (Vol. 2, pp. 3-23). Singapore: Springer.

Yang, F., Jiang, H., Ma, K., Hegazy, A., Wang, X., Liang, S., Chang, G., Yu, L., Tian, B., & Shi, X. (2024). Genomic and phenotypic analyses reveal Paenibacillus polymyxa PJH16 is a potential biocontrol agent against cucumber Fusarium wilt. Frontiers in Microbiology, 15, 1359263.

and my experiences lmao >_<

[microminutes]

Dr Warhol’s Periodic Table of Microbes

The Small Guide to Small Things

82.  Pb.  Paenibacillus

Absolutely the most awesome, artistic, colorful, inspirational microbes ever! These microbes are the perfect fusion of art and science. On solid media they grow in intricate, swirling, feather-like, maybe even Mandelbrot-like patterns. How?

It’s very complicated. First, they can talk to each other. Second, they can sense where nutrients are. Third, they are motile. Fourth (but not really last, we left out a lot of steps) they put 1, 2, and 3 together to coordinate their efforts and search for food using chemoattractant and chemorepulsive responses. The net result is that they move across the Petri dish and leave beautiful patterns on the culture medium. The best-known artistic species are Paenibacillus vortex and Paenibacillus dendritiformis.

Of course they are much more than pretty doodlers. There are almost 200 species, and they have been isolated from soil, abyssal marine sediment, tidal marshes, plant roots, Antarctica, hot springs, insect-pathogenic nematodes (like our friend #54, Xenorhabdus), the NASA Phoenix Lander clean room (very resistant to ultra violet sterilization)!, murals in an ancient tomb, and rarely from clinical samples.

The Paenibacillus are important in agriculture (some fix nitrogen and are used to inoculate fields, others produce antimicrobial compounds), industry (for extracellular enzymes, used in process ranging from cosmetics to biofuel), and cellulose degradation. Paenibacillus is a major pathogen of honey bees, causing highly infectious foulbrood. If you like eating agar, you’d be happy to know that 2 species share your passion. If you like LG electronic equipment, you’d be happy to know that P. elgii was named after the LG Company, which is where the work-up on the microbe was performed.

This is an amazing organism that every microbiologist, geneticist, and biochemist can sink their teeth into. Art majors too!

The name Paenibacillus means “almost bacillus”, they are Gram positive spore forming rods that measure approximately 0.5 to 1.0 microns wide by 3 to 6 microns long, but with so many species, expect to see individual variation.

P dentriformis image: From Ben Jacob Lab, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=22947774. Thank you Ben!

Get a science poster at https://www.etsy.com/no-en/shop/WarholScience

Copyright 2017 Warhol

[theyuanjourneys]

Paenibacillus polymyxa Culture Transfer Technique

Hiii hope you guys have a nice day :>. So i wanna share something i got from internship about how we gain bacteria again and again and so on...

Paenibacillus polymyxa is the bacteria as biocontrol agent that often we make (already explained before). It was so good for plant againts major type of disease of plant (much a like probiotics for plants). But the question is, while we used them how we obtain it?? is it just already there? actually no. All known bacteria pathogenic or beneficial that has been studied will pass all through technique called subculturing.

Subculturing is technique for transfering microorganism from one medium to another medium and it used routinely in preparing and maintaining bacterial stock culture. Yes, that's right! From stock can be increased to 50, from 50 can be 2500 and so on as long the new sterile medium available.

There were many types of subcultures, each type of medium used also be different, but the principle remains the same. As long i know there were slant-to-broth transfer, broth-to-slant transfer, slant-to-slant transfer, and slant-to-agar deep transfer (Cappuccino & Welsh, 2019). However.... at the internship, mentor and other senior were so creative that we ACTUALLY used bottle of glass -_-, yes i mean it. It was so similar we're using slant-to-slant transfer but slightly modified. What i actually amaze that in this internship it applies 3R (reuse, reduce, recycle) ^.^

It was done by all equipment sterilized with autoclave. Bottle that used are glass from YouC1000 (vitamin beverage) and capped with cotton. Start from pouring our agar media to that bottle only small amount that are not overflowed if put horizontal, but not too small that hard to strike with inoculation loop. We used 100% Nutrient Agar Merck with 20 grams each 1 Liters, usually we just make it enough according how much bottle glass we going to use.

We do inoculation by simple zig-zag single streak with using inoculation loop that already heated red hot and used after 10 secs. After inoculation process done, we going to incubate it for 2x24 hours in 30°C, then stored at 4°C for further use. If we need to make it for bigger scale, the isolate itself then given enough sterile water, shake until all diluted and mixed in the big gallons of media for field application

Reference:

Cappucino, J. G., & Welsh, C. (2019). Microbiology: a laboratory manual (12th ed.). Pearson.

[catgirlkirigiri]

I love the wave of chicken nugget posts he seems like a cool guy

He is a cool guy he’s just also a very stupid but cool guy who is currently missing somewhere in the woods next to a 55mph road at 11:33pm

[mecanosaurio]

View this post on Instagram

el rumbo del movimiento #mediaLabPrado #interspecifics #keymerLab #paenibacillus

[rxsarcasm]

My bacteria has a tumblr!!!

So the beginning of the Fall semester in Microbiology I was given a bacteria to research and write 4 papers on and at the end of the semester give a presentation on. When my instructor was giving the directions for the presentation he opened it up for us to be as creative as we wanted, even noting that a student the year prior had made an Eharmony profile for their bacteria. Well I'm highly competetive so this information to me meant I could just do any old powerpoint. I had to get fancy. And so the bacteria tumblr idea was born.

Now granted I only gave myself about 3 days to pull this all together and I only get 5 minutes to talk so it still flows more or less like a powerpoint, it is a presentation after all. But I am most definitely get a 100 on this for sheer awesomeness!!!

http://paenibacillusdendritiformis.tumblr.com/

[paenibacillusdendritiformis]

Discovery

Discovered in early 90's by Eshel Ben-Jacob et al.

Originally classified as Bacillus then reclassified in 1993

Found in soil, rhizosphere, vegetable matter, and insect larvae

Increasing interest due to possible importance in industry,  agriculture and medicine.

[paenibacillusdendritiformis]

Morphology

Paenibacillus = almost rod-shaped

Dendritiformis = tree shaped

Spiral patterns 

   -T Morphotype- tip splitting

   -C Morphotype - Chiral, not identical to its mirror image