I think one of the really interesting ways to differentiate your characters is to play with their motivations and their approaches to doing good, in whatever form that looks like.
Some characters may not care about doing good, in whatever form that might take, but in a lot of fantasy/sci fi, murder mysteries/suspense/romantic suspense, etc. there is some push for at least some of the characters to do good in some form or fashion (e.g., saving someone, solving crime, stopping a bad guy).
Not everyone want to make a positive change for the same reason, and this motivating drive can give a lot of flavor to your character.
A common version that I see particularly in fantasy is a sort of vague "this is my responsibility" or "I am chosen to do this and so I must" but there are a ton of options for what is driving characters to do good:
Belief that doing good is required to get into heaven (or equivalent)
Belief that balancing out prior bad deeds with good deeds is required to get into heaven (or equivalent)
Belief that doing good is a personal obligation that people have to their community/world
Belief that balancing out or mitigating prior bad deeds with good deeds is a personal obligation that people have to their community/world
Desire for fame/adoration
Desire to protect specific person or people
Desire to protect themselves from the bad that they are stopping
Belief in the specific action or policy being implemented
Desire to make the world better because they will benefit from a better world
Desire to make the world better because their offspring/descendents will benefit from a better world
If three characters are working to throw an evil dictator, one might be doing it because they are sick of living under the evil dictator, one might be doing it because they think they can be the next leader if the evil dictator is gone, and one might be doing it because they want to be in the history books as someone who overthrew the evil dictator.
Characters can also have multiple motivations for it--it doesn't need to just be one motivation per character.
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