The Brown Notebook

Meep? Moop. Moop? Meep! Meep? Meep? Meep...

[asajjvxntress]

John is so Tired.

[endangered-justice-seeker]

This isn’t a “border militia” and they’re not “detaining” or “arresting” people. This is a white nationalist terrorist group kidnapping desperate people. Shit

[tatakaeya]

HAPPY EASTER

[feministism]

[pixarsource]

How far computer animation has come: Toy Story (1995) // Toy Story 4 (2019)

[medxclusive]

Introduction to Genome Editing using CRISPR/Cas9

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A post shared by medXclusive (@medxclusive) on Apr 20, 2019 at 6:08pm PDT

Genome editing (also called gene editing) is a group of technologies that give scientists the ability to change an organism’s DNA. Several approaches to genome editing have been developed. A recent one is known as CRISPR-Cas9, which is short for clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9.

CRISPR-Cas9 was adapted from a naturally occurring genome editing system in bacteria. The CRISPR-Cas9 system works similarly in the lab. Researchers create a small piece of RNA with a short “guide” sequence that binds to a specific target sequence of DNA in a genome. The RNA also binds to the Cas9 enzyme. As in bacteria, the modified RNA is used to recognize the DNA sequence, and the Cas9 enzyme cuts the DNA at the targeted location. 

Although Cas9 is the enzyme that is used most often, other enzymes (for example Cpf1) can also be used. Once the DNA is cut, researchers use the cell’s own DNA repair machinery to add or delete pieces of genetic material or to make changes to the DNA by replacing an existing segment with a customized DNA sequence.

[wonderwall]

We finally figured out what makes Kristen Stewart smile: hot wings! The “Twilight” star posed for this amazing photo after dining at a Hooters restaurant in Texas recently. Get the details at Wonderwall.com.

[dietdiscourse-deactivated202211]

yeah, hot wings, sure

[gondwana]

wlw stands for Women Loving hot Wings

[1tenderbeing]

Me when I see hot wings

[ithotyouknew2-deactivated201710]

Just gals loving hot wings

[ninenine-brooklyn]

[knownhag]

chelsea peretti’s opening monologue at the tenth annual tech crunchies

[gothcostco]

what are you even supposed to do on twitter

every time i use it i get angry at someone every 40 seconds

[gothcostco]

twitter is like youtube comments without the videos

[thebrownnotebook]

This is the best thing I've ever read

[gothcostco]

what are you even supposed to do on twitter

every time i use it i get angry at someone every 40 seconds

[gothcostco]

twitter is like youtube comments without the videos

[dappermouth]

Still, you would have loved me only if I had been something holy.

[historical-nonfiction]

A Cute Accident

Clay brick with a stamped cuneiform inscription and a dog’s pawprints.

From the ziggurat at Ur, 2112 BCE - 2004 BCE.

[sovietpostcards]

Pictorial book of the Soviet Union from a 1931 book

[supersgirls]

doctor who series eleven ≡ one gifset per episode → the ghost monument

[supersgirls]

doctor who series eleven ≡ one gifset per episode → the ghost monument

[queerasfact]

This is secret code used by 19th-century diarist Anne Lister to record her lesbian relationships! And underneath, and sample of her diaries. Anne wrote 6600 pages, or almost 4 million words of these diaries, giving us a treasure trove of information about her life, and one of the only first-hand accounts we have of female same-sex relationships in the 19th century.

Now you too can communicate with your friends in secret lesbian code!

To learn more about Anne, check out our episode and follow-up Christmas special!

[theprettiestboy]

You too can write like a vintage lesbian aristocrat