#dna technology

[sezarshop]

DNA helix Sticker by Sezars

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[delightfully-difficult-pirate]

NERD RANT AHEAD

Okay, so I’m rewatching A Discovery of Witches and my nerd brain is laughing my ass off at this computer screen.  

This is from Episode 5, about 27:44 minutes in.  Matthew has just been in the lab, being all sciency, and compared “the actual physical data” from Diana’s DNA to that of their pre-eleventh century witch DNA. 

But on one side of the computer screen he is looking at... is a picture of a Southern Blot, a way of visualizing DNA that was previously separated via a gel matrix (Which Matthew was sloppily preparing at about 26:30).

This technique is OLD.  It was the first technique, I believe, developed to visualize DNA fragments, in 1975.  Usually, the DNA is first fragmented with Restriction Enzymes that look for specific DNA sequences.  

(This type of DNA analysis is also what was used in early Forensic DNA, most notably the OJ Simpson Trial.)

Now, Southern Blots still have it’s place in DNA labs, of course.  But If you’re using a gel to visualize DNA it has mostly been replaced with more modern  techniques that don’t involve radioactive isotopes. 

I work with Mitochondrial DNA like our dear Professor Clairmont does (Why is a biochemist working with Mito DNA????).  I spend 40 hrs a week sequencing ~ 1,200 of its ~16,569 base pairs and analyzing the data.   

Below is what the most common DNA sequencing technique’s data looks like.   Lots of pointy peaks in different colors.  Gives you a headache after a few hours, trust me.

The newest technology (called Next Generation Sequencing even though it’s like the 4th generation of sequencing technology) produces data, that when visualized by a computer program, looks like the colorful strings of letters below.

Someone needs to update movie/show makers and tell them that DNA technology has changed in the past 30 years...  Stop flashing around Southern Blots like they are the height of Genetic research!

Just go get the people who made the Mr. DNA sequence from Jurassic Park, those bastards were ahead of their time!  OR GOOGLE SOMETHING!

Fun science fact or Why Scientists Shouldn’t Be Allowed to Name Things:

The Southern Blot method was developed by Edwin Southern, hence the name.  

When the same basic method was applied to proteins, it was named the Eastern Blot... and then came the Western Blot, which did the same for RNA...  

[theshortnews]

🔥DNA analysis reveals identity of jawbone found on Washington coast 30 years ago

Human remains discovered decades ago along the Washington coast have been identified using advanced DNA technology, marking the second recent identification ... read full news

[frnwhcom]

Unraveling the Mystery: The Resolution of the JB Beasley and Tracie Hawlett Case

In the quiet town of Ozark, Alabama, the unresolved murders of JB Beasley and Tracie Hawlett haunted the community for over two decades. This article delves into the chilling details of their disappearance, the long journey of the investigation, and the breakthrough that finally brought closure to a case that seemed destined to remain unsolved. Disappearance and Discovery On July 31, 1999, JB…

Unraveling the Mystery: The Resolution of the JB Beasley and Tracie Hawlett Case

[michigantopnews]

Coffee Cup DNA Leads to Justice in 25-Year-Old Michigan Rape Case

orensic genealogy solved a 25-year-old Michigan rape case, leading to the conviction of Kurt Rillema. Victim bravery and DNA evidence delivered long-awaited justice.

Forensic breakthrough with coffee cup DNA and victim’s courage bring closure to a decades-old case in Oakland County. DNA Evidence Cracks Decades-Old Assault Case in Michigan Kurt Rillema PONTIAC, Mich. – Justice has finally been served in a 25-year-old rape case after Kurt Rillema, 52, of West Bloomfield, Michigan, was sentenced to 10–15 years in prison for the 1999 sexual assault of a young…

Coffee Cup DNA Leads to Justice in 25-Year-Old Michigan Rape Case

[studychemistrytopics]

What is Recombinant DNA Technology?

Recombinant DNA technology (rDNA technology) is a process that is used for producing artificial DNA through the combination of different genetic materials (DNA) from different sources.

[simplyforensic]

Advancements in DNA Technology

DNA technology has come a long way since its discovery in the 1950s. Today, it is used in various fields, including medicine, forensics, and agriculture. In addition, the advancements made in DNA technology have revolutionized the way we approach problems and find solutions. In this article, we will discuss some of the most significant advances in DNA technology. CRISPR-Cas9 Gene…

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[dakotayounger]

Ancient DNA From 1 Million Years Ago Discovered in Antarctica

As we're a species with ever-shrinking attention spans, it can be difficult to comprehend just how long life has been around on Earth.