#gram-negative

[er-cryptid]

Patreon | Ko-fi    

[aphidcluster]

Crown gall disease of willow (Salix spp.) is caused by Agrobacterium tumefaciens bacterium.

[microscope-world]

This is Bordatella Hemophilus Pertusis - the bacterium that causes whooping cough captured under the microscope at 40x.

[er-cryptid]

Gram Staining

-- invented by Hans Christian Gram in 1884

-- helped distinguish groups of bacteria

-- identifies cells based on their ability to absorb certain dyes

-- gram-positive = cell does not remove the dye

-- gram-negative = cell does not hold dye, and is stained with a counterstain

-- cell walls that absorb the dye are made of peptidoglycans

-- alcohol does not remove this dye

-- lipopolysaccharides do not hold the dye

-- these are bleached with alcohol easily

-- gram-negative examples      -- pseudomonas      -- legionella      -- hemophilus

-- gram-positive examples      -- staphylococcus      -- clostridium      -- listeria

Patreon | Ko-fi

[oaresearchpaper]

Screening, identification and antimicrobial activity of mycoparasitic fungus (Aspergillus sp.) from Philippine aglibut sweet tamarind

Due to the increasing resistance of pathogenic microorganisms, Aspergillus sp. isolated from Aglibut Sweet Tamarind’s bark of Pampanga State Agricultural University was evaluated for potential mycoparasitism and antimicrobial activity. The micrograph obtained from Scanning Electron Microscopy (SEM) Analysis reveals that Aspergillus sp. is a potential mycoparasite; further, its identity was 99% which was confirmed through 18s rDNA of its ITS1 forward and ITS4 reverse sequences by Polymerase Chain Reaction (PCR) Amplification and Sequencing. Moreover, Thin-layer Chromatography (TLC) was used to identify the bioactive compounds of Aspergillus sp. The chemical groups such as Glycosidic flavonoid, Alkaloid and Anthrones were also present which can express the desired activity. Complete Randomized Design (CRD) was carried out with the following treatments; T1 (suspensions), – control (DMSO) and + control (streptomycin for bacteria: ketoconazole for fungus). Paper-disc Diffusion confirms that the suspensions of Aspergillus sp. have significant antimicrobial potential as shown in the zones of inhibition in S. aureus and S. cerevisiae but with lower activity in E. coli. Thus, Aspergillus sp. is a potential mycoparasite and source of new drugs and drug products.

[oaresearchpaper]

Identification of tolerance in Rangpur citrus against huanglongbing

Rangpur citrus group was tested during the present study for the identification of tolerance against huanglongbing. Control of huanglongbing (HLB) disease is necessary around the world to avoid the complete destruction of citrus. Rangpur accessions were graft challenged with huanglongbing positive budwood to study the resistance and symptom expression. Vein yellowing symptoms appeared on Rangpur poona nucellar and tuningmeng nucellar while vein yellowing and blotchy mottle symptoms appeared only on tuningmeng nucellar. Kirumakki nucellar and knorr nucellar expressed no symptoms. Being a drought-tolerant rootstock with less HLB symptom expression, Rangpur rootstock cultivars especially kirumakki nucellar and knorr nucellar may be used to test the production and survival of plant with compatible scion varieties.

[vinnieworld]

The Role of Iron in pathogenic gram- negative bacteria; Neisseria meningitidis

Author: Bharathi Kavindi Jayaratne AbstractIron is essential for pathogenic growth and colonisation. Hence, it plays an important role in infections caused by bacteria. Pathogens such as bacteria isolate iron from the host through various molecules and receptors, allowing the bacteria to survive the host’s immune responses and defense mechanisms. Which the growth, replication, metabolism, and…

View On WordPress

[vinnieworld]

The Role of Iron in pathogenic gram- negative bacteria; Neisseria meningitidis

Iron is essential for pathogenic growth and colonisation. Hence, it plays an important role in infections caused by bacteria. Pathogens such as bacteria isolate iron from the host through various molecules and receptors, allowing the bacteria to survive the host’s immune responses and defense mechanisms. Which the growth, replication, metabolism, and virulence of the pathogen. Due to…

View On WordPress