Unsounded is an amazing comic with wonderful characters and dialogue. I have read it one and a half times now, and I think I have found satisfactory answers to a very important question. What are the characters' opinions on trans people?
Duane Adelier: I think he would be supportive of trans people if he met and talked to one. He would still need a bit more convincing to accept non-binary genders though, and he'd probably still be casually misogynistic.
Sette Frummagem: She would probably be supportive but would also make transphobic jokes occasionally, no matter what boundaries any individual trans people have set. I'd put the rest of the Frummagem family in this category, with varying degrees of disrespect, except from Anadyne, who i think would be just supportive, as she is not interested in petty bouts of one-ups-manship.
Mathis Quigley: I don't think he cares for trans people. But only because he is generally misanthropic, not because he is particularly bigoted. Nevertheless, I wouldn't expect any allyship from him.
Matty: He's a sweet baby boy. He would definitely be supportive.
Jivi: He would be supportive, but he would be prickly about it initially. Like he might get one's name or pronouns wrong and when they correct him he'd get mad, but I think that eventually he would get over it and be normal about it.
Emil Toma: There is a point where Mathis debates Duane's humanity but Emil is like "you say your name is Duane and I accept it and that's how I'll treat you". So I think he would be supportive of trans people. If a trans person committed crimes against the crescian queen, Emil would definitely use their correct name and pronouns as he arrested them.
Elka: She would be generally positive, but she would misgender a trans person she thinks did something bad.
Bastion Winalils: Supportive, but he may or may not be a chaser.
Queen Sonorie: Of course she is supportive of trans people. However, her plans as a politician do not include them, as she believes she has much more pressing matters to attend to.
General Bell: If Bell lived in the current real world he would be a youtuber that made videos about being an alpha male, so I'm pretty sure he would be a transphobe.
Cutter: Transphobic, but only because he hates everyone already.
Starfish: Transphobic. He would commit hate crimes.
Murkoph: Supportive. He would commit hate crimes.