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Mastalgia: Etiopathogenesis and Management: A 2 Year Experience
Background: Mastalgia is breast pain and can vary in intensity from a mild tingling to a sharp pain. Objective: The purpose of this study was to determine the side incidence and age group affected in females presenting with mastalgia and present evidence-based concepts to provide with an overview of diagnosis and treatment. Methods: A total of 71 patients were included in the study and age and side incidence of patients with breast pain were investigated. Results: A total of 90 patients aged 31-60 years who presented with breast pain were followed up. Most patients presenting with mastalgia fell between the age group of 41-60 years and complained of cyclical, bilateral mastalgia. Conclusion: Mastalgia is the most frequent symptom of breast diseases and the fear that breast pain is a symptom of breast cancer and the presence of severe pain that affects a woman’s quality of life make it an important health issue worth discussing. Stress and anxiety play a crucial role in pathogenesis of mastalgia and reassurance, breast support brassiere, and topical NSAID gel massage form the initial management of mastalgia. Danazol, Bromocriptine, Evening primrose oil and Vitamin E have been seen to be effective in the treatment of mastalgia.
Keywords: Mastalgia; Cyclical mastalgia; Noncyclical mastalgia; Danazol; Bromocriptine; Evening primrose oil; Vitamin E; Flaxseeds
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Introduction
Mastalgia is the most common breast symptom in patients attending a breast clinic [1]. 60 to 70 % of women experience some degree of breast pain at some stages of their lives, and in 10 to 20 % of cases, it is severe [2]. Mastalgia can be commonly associated with premenstrual syndrome, fibrocystic breast disease, psychological disturbance and, rarely in breast cancer [3]. Mechanical breast support, a low fat and high carbohydrate diet and topical nonsteroidal anti-inflammatory agents are reasonable first-line treatments. Hormonal agents such as bromocriptine, tamoxifen and danazol have all demonstrated efficacy in the treatment of mastalgia [3].
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Aim
Breast pain is a common symptom and the concerns of patients presenting with mastalgia are the fear that breast pain is a symptom of breast cancer and the presence of severe pain that affects a woman’s quality of life. The purpose of this study was to determine the side incidence and age group affected in females presenting with mastalgia not responding to treatment with non-steroidal anti-inflammatory drugs and present evidence-based concepts to provide with an overview of diagnosis and treatment.
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Materials and Methods
It is a prospective study of Mastalgia, wherein a group of females presenting with symptoms of breast pain were evaluated clinically and radiologically, treated and followed up till the symptoms were relieved. Only Ultrasonography of the breast was used as the imaging modality. All the participants in the study gave their informed consent and knew that objective of the Ultrasonography study was just to screen for a breast lump. Any patient who was found to have a breast lump on sonological examination was excluded from the study. The patients were routinely examined by the Lady Medical Officers and all the females with mastalgia were encouraged to report initially to the Lady medical officer who initially treated them with non-steroidal anti-inflammatory drugs were referred to the department of Surgery for further management only if there was no symptomatic relief after a week. The patients had free access to the Lady Medical Officer and were always encouraged to report about the nature, severity and timing of breast pain in relation to their menstrual cycle.
A total of 71 patients were included in the study. We investigated age and side incidence of patients with breast pain. Inclusion criteria included the patient having unilateral or bilateral cyclical breast pain. The characteristics of pain warranting inclusion in the study were tingling or mild pain which affected quality of life to severe unilateral or bilateral breast pain usually a week before the patient has her periods and pain reducing in severity after her periods. Patients who were excluded from the study were with a history of breast lump, nipple discharge, redness, breast retraction and pregnant and lactating women, known cases of breast cancer who have undergone surgery, patients with history of costochondritis, trauma and patients with cardiac and respiratory ailments
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Results
During the 2-year period between Jun 2017 to Jun 2019, a total of 90 patients aged 31-60 years who presented with breast pain were followed up.
Age group affected
16 patients (17.77%) were between the age group of 31- 40 years, 45 patients (50%) were between 41-50 years and 29 patients (32.22%) were in the age bracket of 51-60 years (Figure 1). Most patients presenting with mastalgia (74 out of 90: 82.22%) fell between the age group of 41-60 years, 51 patients (56.66%) were evaluated to have mild pain, 29 patients (32.22%) had moderate pain and 10 patients (11.11%) complained to have severe pain.
Side incidence
63 patients (70%) complained of bilateral mastalgia, 16 patients (17.77%) had left sided breast pain and 11 patients (12.22%) complained of pain in the Right breast (Figure 2).
Cyclical versus non-cyclical mastalgia
54 patients (60%) complained of cyclical mastalgia whilst 36 patients (40%) presented with noncyclical mastalgia (Figure 3).
Severity of pain
Breast pain described by the patients was divided into mild, moderate and severe. Mild pain was pain lasting for about 15-20 min in a day and lasting for 1 week. Moderate pain was defined as pain lasting for 1-2 hours in a day and extending up to 2 weeks whereas severe pain was described as persistent breast pain for more than 2 weeks. 51 patients (56.66%) were evaluated to have mild pain, 29 patients (32.22%) had moderate pain and 10 patients (11.11%) complained to have severe pain (Figure 4).
Age of menarche
8 patients (8.88%) had menarche at the age of 11 years, 39 patients (43.33%) had menarche at 12 years of age, 37 patients (41.11%) at the age of 13 years and 6 patients (6.66%) at the age of 14 years (Figure 5).
Menopause 32
Patients (35%) out of 90 patients who presented with mastalgia had attained menopause. Relationship with the menstrual cycle in cyclical mastalgia: 54 out of 90 patients described their pain to be cyclical out of which 36 patients (66.66%) complained of mid cycle pain lasting for about a week, 10 patients (18.51%) revealed that they had mastalgia between 2nd and 3rd week of the menstrual cycle and 8 patients (14.81%) said that they had breast pain 1 week before having their periods and the pain reduced in intensity after their periods
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Discussion
Mastalgia occurs most common in women aged between 30- 50 years 4 and is generally bilateral, diffuse, poorly localized, and generally described as a heaviness or soreness that often radiates to the axillae and arms [5]. There are contemplates which point towards a connection among mastalgia and mental stress [6]. Ader et al. [7] revealed that expanded caffeine utilization and smoking were related with mastalgia. Expanded estrogen levels, diminished progesterone levels, inconsistencies of the estrogen progesterone proportion, or expanded prolactin levels are related with mastalgia [8]. The cyclic nature of the breast pain, the presence of breast symptoms such as swelling, tenderness, and nodularity, and the cessation of these symptoms in the postmenopausal period are presumably because of the impacts of the estrogen hormone [9]. Mastalgia may occur due to fatty tissue necrosis, or a strain in the Cooper ligaments as results of either blunt or penetrating trauma to the breast [10]. An association between mastalgia and increased breast density has been accounted for [11]. Benign breast disorders are significantly associated with mastalgia [12] and public awareness of breast cancer has led to a steady flow of frightened females attending surgical outpatient clinics, with complaints of cyclical mastalgia, nodularity, or asymmetry, but a small proportion will indeed present with breast lumps [13].
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Etiology
Expanded estrogen emission from the ovary is the most acknowledged theory of mastalgia. An examination from France demonstrated a fundamentally decreased degrees of progesterone in patients with mastalgia [14]. Peters et al. [15] discovered that the patients with mastalgia had a fundamentally more noteworthy ascent in prolactin contrasted and the controls. At the Cardiff Mastalgia Clinic, the total body water was estimated utilizing radioactive water in mastalgia patients and asymptomatic ordinary ladies and another speculation was put across that simple retention of body water may be associated with breast pain [16]. Astley Copper proposed that mastalgia patients were neurotic [17]. Minton et al. [18] announced that caffeine confinement delivered improvement in symptoms. Peters et al. [19] observed that breast pain was related decidedly with level of ductal dilatation, as shown by ultrasound studies
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Investigation
Patients are explained about hormonal changes with menstruation and its relationship with mastalgia. A pain dairy is maintained by the patient and they record their daily pain experience on a chart. The severity of pain is also noted on a visual analogue scale (VAS) where 0 indicates no pain and 10 indicates very severe excruciating breast pain. Most experts consider any pain of ≥3 on a VAS of 0 to 10 to be significantly severe to require therapy [20]. Ultrasonography of the breast may be done to rule out any sub clinical lump and a mammogram may be performed for women above 35 years of age to see for cysts and other benign lesions which might be detected on a Mammogram.
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Treatment
The majority of patients can be managed by exclusion of cancer and reassurance [21]. Anxiety and stress are significant components prompting mastalgia and anxiolytics have been concentrated to have a job in patients with mastalgia associated with anxiety and depressive symptoms. Danazol is the most effective drug in the treatment of cyclical and noncyclical mastalgia with Bromocriptine and Evening primrose oil having equal efficacy [21]. Danazol is given in the dose of 200mg /day on the second day of menstrual cycle for 2 months followed by 100mg/day for the next 2 months. Bromocriptine is started at a dose of 1.25mg /day and may be gradually increased up to 2.5mg/ day and continued for a period of 2 months. Evening primrose oil is given in the dose of 3gm/day for a period of 4-6 months. Evening primrose oil, which contains omega-6 essential fatty acids and its effective component is believed to be gamma-linolenic acid [22]. Vitamin E has also been used in treatment of mastalgia effectively. Daily doses of 1,200 IU vitamin E and 3,000mg EPO in combination taken for six months may decrease the severity of cyclical mastalgia [23]. Flaxseed with the scientific name Linum usitatissimum contains omega-3 fatty acid and through it, the production of some arachidonate metabolites are reduced, and eicosanoids derived from omega-3 found in flaxseed, have antiinflammatory properties [24]. The study of Alvandipour et al. [25] showed that evening primrose oil and Vitamin E have similar therapeutic effects in reducing breast pains. Primrose oil capsules are also available and are given in the dose of two 1000mg capsules daily whereas Vitamin E is given as 400 IU capsule daily for a period of 2 months. Inverse relationship between mastalgia and breast cancer: Women who experience breast pain are less inclined to be determined to have breast cancer than women who do not complain of breast pain [26]. Despite the fact that mastalgia isn’t viewed as an indication of malignancy in essence, its presence does not rule out the presence of cancer, either. A study by Arslan et al. [27] uncovered that 0.2% of the women with mastalgia were diagnosed with breast cancer.
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Conclusion
Mastalgia is the most frequent symptom of breast diseases and the fear that breast pain is a symptom of breast cancer and the presence of severe pain that affects a woman’s quality of life make it an important health issue worth discussing. Stress and anxiety play a crucial role in pathogenesis of mastalgia and reassurance, breast support brassiere, and topical NSAID gel massage form the initial management of mastalgia. Danazol, Bromocriptine, Evening primrose oil and Vitamin E have been seen to be effective in the treatment of mastalgia. The most common age group affected is between 41-50 years and breast pain involving both breasts is the commonest. Cyclical mastalgia is commoner than non-cyclical mastalgia and mild pain which is described as pain lasting for about 15-20min in a day and lasting for 1 week affected most patients suffering from mastalgia. Most patients complained of mid cycle pain lasting for about a week. Early menarche and late menopause may be associated with mastalgia and the most common age of attaining menarche in patients who reported with mastalgia was at 12 years of age. Women who experience breast pain are less likely to be diagnosed with breast cancer and 0.2% of the women with mastalgia were diagnosed with breast cancer.
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Effects of Unsaturated Fatty Acids in Attention Deficit Hyperactivity Disorder
Long-chain polyunsaturated fatty acids (PUFA) in the blood, especially omega-3 fatty acids, have been repeatedly associated with various behavioral disorders, including ADHD, but the relationship has not yet been fully established. On the other hand, treatment of low omega 3 and PUFA levels with nutritional supplements in children with neurodevelopment disorder such as ADHD, dyslexia and dyspraxia are thought to improve disease symptoms. This review presents a summary of effects of unsaturated fatty acids in attention deficit hyperactivity disorder
Keywords: ADHD; Unsaturated Fatty Acids; Omega 3; Omega 6
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Introduction
Nutritional status and eating behaviors of children with ADHD
Attention Deficit Hyperactivity Disorder (ADHD) is defined as a neurodevelopmental disorder that reflect the persistence of ADHD symptoms such as inattention, overactivity, and impulsivity across lifespan [1]. ADHD was associated with greater severity of global eating disorder pathology, restraint, eating, shape, and weight concerns. Several mechanisms explain the reason of ADHD on eating patterns. According to a recent study (2018), children with ADHD may lose control more while eating and may consume more calories than healthy subjects. In addition, children diagnosed with ADHD may eat more food even if they are satiated compared to healthy subjects [2]. Faster eating in children with ADHD, inability to focus on hunger-satiety cycles, and inability to perceive body stimuli may lead to impaired eating-feeding patterns
As ADHD is a common diagnosis all over the world, experts and families are turning to different methods of treatment which have also associated with nutritional status. These methods are increasing and sometimes cause controversy due to the fact that there are some methods which are not proven yet. Although the approach to the treatment of ADHD with fatty acid supplementation is a frequently studied topic, there is a lack of a comprehensive literature review.
Unsaturated fatty acids in attention deficit hyperactivity disorder
Long-chain polyunsaturated fatty acids (PUFA) in the blood, especially omega-3 fatty acids, have been repeatedly associated with various behavioral disorders, including ADHD, but the relationship has not yet been fully established. On the other hand, treatment of low omega 3 and PUFA levels with nutritional supplements in children with neurodevelopment disorder such as ADHD, dyslexia and dyspraxia are thought to improve disease symptoms [3]. Furthermore, no significant adverse effects have been associated with this treatment model. In addition, some studies suggest possible beneficial outcomes in case of co-morbid conditions such as epilepsy [4].
According to a study, the ratio of omega-3 fatty acids was lower in plasma phospholipids and erythrocytes in ADHD group than in control group, whereas saturated fatty acid ratios were higher in ADHD group than in control group. While saturated fat intake was 30% higher in ADHD group, intake of all other nutrients was not different [5]. Studies have shown that omega-3 levels are lower in people with ADHD compared to their unaffected counterparts, but the reasons for this are not fully known [6].
In a study examining lipid and lipoprotein profiles, plasma triacylglycerols and phospholipids were lower and free cholesterol, HDL and apo-AI were higher in ADHD patients than controls. When compared to controls, total saturated fatty acid ratio was higher and PUFA levels were lower in ADHD patients. Therefore, this resulted in a significant reduction in the ratio of PUFA/saturated fats. It has been reported that lipid peroxidation is reduced (decreased plasma malondialdehyde values, increased G-tocopherol concentrations) in ADHD patients. The results of the study reveal that there are significant changes in lipid and lipoprotein profile and oxidant antioxidant status of ADHD patients [7]. These data indicate that essential fatty acid profiles of children with ADHD are abnormal, and this abnormality is not due to food intake.
Omega-3 fatty acid contains Elkosapentaenoic acid (EPA) and decosahegzaenoic acid (DHA). DHA omega-3 fatty acid accounts for more than 90% of omega-3 fats in the brain. Recent reviews show that EPA has more clinical efficacy. However, both EPA and DHA are expected to be important, based on methodological considerations of the included studies, the majority of studies to date, and different mechanisms of action [6].
High-dose EPA/DHA supplementation can improve behavior in children with ADHD. In a randomized controlled trial [8], children with ADHD showed increased EPA, decreased omega 6 fatty acid, and clinical improvement as a result of 0.5g of EPA supplementation for 15 weeks. Alternatively, there is a multifaceted complex interaction between further deterioration in executive functions, eating-appetite problems, eating problems, obesity, nutritional status, attachment and family relations problems and ADHD symptoms
According to a study, omega-3 fatty acid supplementation was found to have a small but significant effect on the improvement of ADHD symptoms, and the dose of eicosapentaenoic acid in food supplements was significantly correlated with supplementation efficacy. Omega-3 fatty acid supplementation was particularly effective in treating ADHD with higher doses of eicosapentaenoic acid [9]. Also, a recent meta-analysis shows that PUFAs supplementation monotherapy improves clinical symptoms and cognitive performances if these youths have a deficiency in n-3 PUFAs levels [10].
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Controversial Studies
A recent Systematic Review [11] concluded that the majority of data showed no benefit of PUFA supplementation, although there were some limited data that did show an improvement with combined omega-3 and omega-6 supplementation. But they explained this conclusion via small sample sizes, variability of selection criteria, variability of the type and dosage of supplementation, short follow-up times and other methodological weaknesses. However, a recent meta-analysis of 24 studies with ADHD reported by Cooper et al showed that did not show improvements in any of the cognitive performance measures [12].
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New Perspective
In this literature review, we emphasize the need for additional and more extensive clinical studies, but we concluded that omega-3 fatty acid supplementation is useful in the treatment of ADHD. It is thought that it will be beneficial to analyze the effects of food supplements by comparing them with different food supplements in the planning of treatment. In addition, ADHD, which adversely affects the quality of life, is important in terms of developing developmental problems from childhood to adulthood, using early diagnosis and effective treatment methods. Children and families should be properly informed during the treatment process. In order to raise the awareness of the families about nutrition and food supplements, programs can be included in social media channels, visual media and television where the studies on PUFA are explained and their effects are evaluated. The effectiveness of PUFA is thought to increase the use of food supplements to support pharmacological interventions, affect the course of treatment
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Conclusion
This review gives an introduction to recent findings on the clinical efficacy of PUFAs in ADHD. Studies, Systematic reviews and meta-analyses of randomized controlled trials (RCTs) assessing outcomes of supplementation with PUFAs have shown inconsistent results. But literature provides further evidence of the beneficial effect of supplementation with PUFA in the treatment of ADHD. Future research should further explore abnormal fatty acid metabolism in ADHD.
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Ethnomedicines and Health Management in Kenya: Which Way Forward?
Ethnic diversity and ethnosystematics have gradually evolved to give rise to unique cultures and ethnomedical practices and systems that have withstood the test of time worldwide. An estimated 70% of Kenyans rely on ethnomedicines as the only source of healthcare, with up to 90% using medicinal plants and animals as part of any treatment process. WHO estimates that as many as 70-95 % of the world’s people especially in developing countries rely on ethnomedicines for their primary healthcare needs and henceforth its recommendation in the Alma-Ata Declaration adopted by WHO and UNICEF in 1978 for its integration into primary healthcare systems, particularly of developing countries if the objective of the “Health for All by the Year 2000” was to be realized [1]. While about 100 million people use ethnomedicines in the European Union (EU) alone while more than one third of Americans and Europeans use assorted herbs for healthcare purposes, spending over £7.0 billion annually. On other hand, 1.5 billion people use Chinese Tradition Medicine worldwide and most modern conventional drugs have their origin of use and application deeply rooted in ethnomedicines. There is need therefore to understand the holistic nature of ethnomedicines so that it can be easily utilized and applied. This overview focuses on the composition and understanding of ethnomedicines, its historical development in Kenya, its challenges and finally suggests a way forward of unlocking its potential together with anticipated benefits. An illustrated example of unlocking potential of ethnomedicines is briefly given for Maasai Mara University with reference to its African Medicinal Botanical Garden under development.
Keywords: Ethnophamacology; Conventional medicine; Legal framework of ethnomedicines; Healthcare systems; Ethnopractitioners
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Introduction
Ethnic diversity and ethnosystematics have gradually evolved to give rise to unique and beneficial cultural and ethnomedical practices that have withstood the test of time in various human societies worldwide. The prospect of the sustainable growth, development and evolution of these ethnopractices (including social, cultural and religious practices) has been achieved due to a rich biological diversity closely associated with diversified ethnicity in which each existing ethnic grouping contributes a unique and yet useful ethnopharmacopoeia [2]. Achieving optimal wellness for humanity together with animals, minerals, soils, water, microorganisms, plants and the entire environment that humans take care, has ever remained the omnipotent challenge since antiquity! The aim of these ethnopractices therefore was to develop a pool of ethnomedicines that approximates to the aspirations and realities of a holistic approach to ill-health condition of humanity and the entirety wellbeing rather than the conventional curative mechanism of technological fix of specific diseases only [3,4]. Moreover, the packaged ethnotreatments take care of the environments in which the target organisms exist, thus making ethnomedicines very unique and indeed fulfilling as ethnopractitioners endeavour to reconnect the individual-based social wellbeing and emotional equilibrium of a target patient whose taboos, norms and other traditions are philosophically considered alongside the ethnic code of conduct, governing set of rules and existing relationships [5]. As Abdullahi [2] stated, in many circumstances, the ethnopractitioners normally and more often than not act as, “an intermediary between the visible and invisible worlds; between the living and the dead or ancestors, sometimes to determine which spirits are at work and how to bring the sick person back into harmony with the ancestors and/or spirits”. Ethnomedicines have proved powerful ingredients in our livelihoods at all levels as a reliable, independent and biologically evolving healthcare system since human existence [6]. For incidence, an estimated 70% of Kenyan’s rely on ethnomedicines as the primary source of care, with up to 90% using animals, minerals, soils, water, microorganisms and plants as part of any treatment process. While World Health Organization (WHO) estimates that as many as 70-95% of the world’s people especially in developing countries rely on ethnomedicines for their primary healthcare needs and henceforth its recommendation for integration into primary healthcare services [5,6]. And further estimates that about 100 million people use ethnomedicines in the European Union (EU) alone while more than one third of Americans and Europeans use assorted herbs for healthcare purposes, spending over £7.0 billion annually. While China estimates that about 1.5 billion people globally use the Chinese Herbal Medicines (CHM) [8,9]. There is also an estimate of over 50% of modern pharmaceutical drugs having their origin of use and technological applications in ethnomedicines, with plant species together with the ethnopractitioners that use them taking the lead as the main resources [10-19]. Nevertheless, the most disturbing picture is that out of an estimated number of about 390, 800 plant species known in the world today, only 15% of these plant species have been investigated for possible medicinal value. How about the medicinal value of more than 1.611 million existing zoological species so far named and described and another estimated number of about 10 to 30 million living organisms that remain to be discovered? This is just amazing and an incredible pharmaceutical resource that is yet to be utilized to benefit humanity worldwide! This aforementioned brief account on ethnomedicines and health management therefore provides an adequate amount of evidence for investment into ethnomedicines research and development with a view to identifying more resources for developing new pharmaceutical agents and adding value to the existing ethnomedical agents and practices in Kenya [20]. This overview of ethnomedicines outlines its composition and understanding, its historical development in Kenya, its challenges and finally suggests a way forward of unlocking its great potential together with anticipated benefits.
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Composition of Ethnomedicines and Names Uniquely Used for Describing and Defining it
Ethnomedicines is uniquely defined and variously described using a wide range of geographic- and ethnic-based terminologies worldwide such as, Ayurveda, Siddha medicine, Unani, ancient Iranian medicine, Iranian (Persian) traditional medicine, Arabic indigenous medicine, Islamic medicine, traditional Chinese medicine, traditional Korean medicine, ancient Greek medicine, Haitian folk medicine, Native American traditional herbal medicine, Uyghur traditional medicine, Irish medical families, Japanese Kampō medicine, traditional Aboriginal bush medicine, Georgian folk medicine, acupuncture, Celtic traditional medicine, Muti, Ifá, native healing powers and traditional African medicine (ancient Egyptian medicine). Other descriptive terminologies of ethnomedicines include: traditional medicine, folk medicine, indigenous medicine, natural medicine, home remedy (granny cure), herbal medicine, integrative medicine, complementary medicine, anthroposophic medicine (massage, exercises, counselling and substances from plants, animals, soils, water, minerals, microorganisms etc.), alternative medicine, pseudo-medicines, magical medical practices, quackery medical practices, homeopathy, skepticism, etc. Ethnomedicine industry is therefore marred with a lot of controversy, doubt, suspicion and skepticism, which do not ensure efficiency, sustainability, effectiveness and timeliness in service delivery in the entire industry, henceforth its failure to grow and develop. This haphazard consideration of the field of ethnomedicines shows that this particular discipline has not grown and developed across board uniformly and that it has not professionally curved its own niche and thus gotten into the mainstream administration of academia and research. However, given that WHO is a specialized agency of the United Nations leading and setting out the course for the use and application of ethnomedicines throughout the world, it has universally developed the most acceptable and favourable definition of ethnomedicines as, “the sum total of the knowledge, skills, and practices based on the theories, beliefs and experiences indigenous to different cultures, whether explicable or not, used in the maintenance of health as well as in the prevention, diagnosis, improvement or treatment of physical and mental illness” [7]. Throughout the entire text of this manuscript, the meanings of the following terminologies have been understood to be in the following context unless otherwise differently explained.
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A Critical Review of Concepts and Definitions of Fundamental Terminologies
Ethno
A prefix used in the formation of compound words in English language to mean the study of races, people, cultures, tribes, caste systems, nations, societal classes and societies with a common and distinctive origin and evolution in terms of language, communication, cultural practices, nutrition, norms, beliefs, taboos, traditions, skills, technologies, innovations and just the entire livelihood from its ancient times to current. Examples include: Ethnomusicologistsomeone who studies the music of different societies and cultures, Ethnocentric-believing that the people, customs, and traditions of your own race or nationality are better than those of other races and Ethnology-the study of different societies and cultures
Ethnography
A scientific description of cultures (including traditional knowledge, ethnopractices and customs of indigenous/native and local people) of a particular society by someone who has lived in it and/or a book containing such information.
Ethnobotany
The scientific study of the traditional knowledge, practices and customs of indigenous/native and local people of a given geographical location and/or ethnic grouping concerning the values, applications and wider practical utilization and interaction of plant species by humanity; including their clothing, construction, ornamental, initiation ceremonies, farming, nutritional, medical, religious and many other aspects of uses in the course of their life [2,21,22].
Herbalism
A traditional medicinal or folk medicine practice based on the use of plants and/or plant extracts intended for medicinal purposes or for supplementing a diet (nutraceutical) with the aim of providing healing effects. Herbalism is also variously known as botanical medicine, medical herbalism, herbal medicine, herbology and phytotherapy.
Phytotherapy
From the Greek, “phyton” meaning “plant” and “therapeuein” meaning “to take care of, to heal,” this is the term used to describe medical herbalism too, is a science-based medical practice of using plant-derived medications in the treatment, management and prevention of diseases based on their empirical evidences of their medical potential and value [23].
Zoopharmacognosy
Is a broad terminology referring to a natural-based behaviour in which non-human animals apparently self-medicate by selecting and ingesting, absorbing or topically applying plants, soils, insects and psychoactive drugs or humans using animals, animal parts and products to prevent and/or therapeutically to reduce the harmful effects of pathogens and toxins as prophylactic and/ or curative measures, respectively [24-27]. This terminology originated from the Greek roots, zoo (meaning, “animal”), pharma (meaning “drug”) and gnosy (meaning “knowing”) and ever since its origin in 1993, it has found its position in the mainstream of administration of academia and research as independent discipline [27-29]. It involves what Caroline Ingraham described in 1984 as “evolution of animals’ behaviour to cope with potential health threats including disease and injury, through the development of solutions to restore health by self-diagnosing and administering medicinal compounds naturally found in the immediate environment.” Nevertheless, Ingraham’s philosophical statement of Applied Zoopharmacognosy has been heavily criticized by the author of The Skept Vet (http://skeptvet.com/Blog/2016/10/the-natural-nonsense- that-is-applied-zoopharmacognosy/).
Geophagy (with ethnomedicinal and nutraceutical values) (also known as geophagia)
The practice, in some world tribal and ethnic societies of eating earthy substances (such as clay) that in humans is performed especially to augment a scanty or mineral-deficient diet or as part of a cultural tradition that is associated with ethnomedicinal value and/or bodily requirements of organisms [30-32]. From different viewpoints, geophagy has been regarded as a psychiatric disease, a culturally sanctioned practice or a sequel to poverty and famine [31,33].
Microorganisms as ethnomedicines
The search for drugs from microorganisms to manage human health and that of other organisms is more recent and not based on ethnomedicinal root records in time and space as is associated with evolutionary history of humans [34]. Probably of particular significance to note is a considerable part of the currently available conventional pharmaceuticals in clinical use being comprised of drugs derived from microorganisms too [13]. Through the practice of geophagy therefore, humans may be consuming a lot of microorganisms unknowingly and henceforth, benefiting greatly from their useful natural compounds available in the soils with curative, protective and preventive properties [32]. It follows with logical necessity that a considerable part of the medicinal value of soils may be attributed to the existence of these microorganisms as soil-based bioresources with medically useful compounds.
Ritual medicines
In this context, it refers to a sequence of activities and/or events practically involving gestures, words (utterances, known as performatives) and objects performed in a sequestered place and according to set sequence of social custom, taboos and/or normal protocol, more often as part of a traditional religious ceremony in performing divine and/or holy services but with a view to getting holistic curative, protective and preventive remedies [35-37]. Ritual is more related to performing religious services as a methodological measure of religiosity level although characterized by formalism, traditionalism, invariance, rule-governance, sacral symbolism and performance [36,38,39]. It is full of genres and anthropological theories that govern human evolutionary processes in the society [40-42].
Complementary medicine (CM)
The terms “complementary medicine” or “alternative medicine” refer to a broad set of health care practices that are not part of that country’s own tradition or conventional medicine and are not fully integrated into the dominant healthcare system. They are interchangeably used with traditional medicine in some countries.
Spirituality
It explores life purpose/meaning and relates to religious conviction, involving deep feelings or beliefs of a religious nature and the quality of being concerned with the human spirit or soul (and/ or mental or emotional communion) rather than the material or physical values or pursuits in life. However, religion and spirituality are not the same thing, nor are they entirely distinct from one another but the two are closely related and may offer questions and answers about the infinite, provide support during emotional crises and invoke a sense of awe, wonder, and reverence. Conversely, life issues relating to comfort, beliefs, reflection, ethics and awe are all common to both religion and spirituality, thus affecting the way one think, feel and behave in the society. Nevertheless, Mastin [43] defined spirituality as, the measure of how willing we are to allow Grace (loveliness)-some power greater than ourselves-to enter our lives and guide us along our way, thus being how loving you are, how unconditionally accepting you are toward yourself and others. Spirituality takes charge of one’s emotional health and wellbeing (a state of being in alignment (body, mind, and spirit)) and refers to the way individuals experience their connectedness, energization, contentment and balancing to the moment, to self, to others, to nature and to the most significant or sacred and/or supernatural powers.
Divinity
In religion sense, divinity or godhead is the state of things that are believed to come from a supernatural power or deity (holy being), such as a god, goddess, Supreme Being, creator deity or spirits and are therefore regarded as sacred and holy. Such things are regarded as divine due to their transcendental origins or because their attributes or qualities are superior or supreme relative to things of the Earth. Divine things are regarded as eternal and based in truth, while material things are regarded as ephemeral and based in illusion. Such things that may qualify as divine are apparitions, visions, prophecies, miracles, and in some views also the soul, or more general things like resurrection, immortality, grace, and salvation. Otherwise what is or is not divine may be accordingly defined based on the ethnicity, beliefs, lifestyle, religiosity levels and at times, personality and environmental situation in one’s life. Above all, supernatural powers and associated holiness and being sacred in divinity make sense in an ordinary life when it provides holistic curative, protective and preventive remedies in ill-health situations in the society [35-37].
Ethnoknowledge systems (such as Ethnobotany, Ethnozoology, Witchcraft, Ethnomedicines, Ethnography etc)
Is geographic and/or ethnic-based traditionally defined complex body of knowledge (information), practices, norms, taboos, cultures and believes that normally guide day-to-day livelihoods of individuals belonging to a specific geographic location and/or ethnic grouping regarding their socio-cultural wellbeing, health, leadership, governance, economic life, relationships, informal education etc. The coherent ensemble of this complex body of knowledge (information), practices, norms, taboos, cultures and believes is build up from a wide range of sources (inherently within and without) in time and space throughout the evolutionary history of that particular geographic location and/or ethnic grouping [44,45]. The holistic nature and interconnectedness of ethnoknowledge systems in human societies in which they exist, make them very unique, to be held in high esteem, independent and as a fundamental resource for growth and development of ever evolving humanity. It therefore follows with logical necessity that the efficiency and efficacy of every defined athenahealth system may be appropriately and favorably measured in a culturally appropriated way, and the failure to consider the existing cultural context and framework within which it operates may result in its misunderstandings and failure to achieve the desired results [46- 48].
Cultural life
Refers to upholding the professional ethics of ethnopractices, ethnomedicines, ethnoknowledge, norms, taboos, cultures and believes of the various components of ethnoknowledge systems within any one given existing cultural context and framework. Culture itself is the sum total of people’s way of life through evolutionary history since antiquity, thus referring to their entire social behaviour, mindset ethnic-based orientations and norms (informal understandings that govern the behaviour of members of a particular society) found in human societies and expressed in all sorts of forms, both physically (objects and architecture they make or have made) and immaterially (values, belief systems, attitudes, rules, norms, morals, language, organizations, and institutions) [2]. Culture also considers values of the people and the ways they think about and understand the world around them and their own lives [49,50].
Ethnosurgery mechanisms and fracture making
Is a highly evolved and specialized form of traditional medicine that breaks bones to remove bad areas and restores bone tissues to their original physical and mechanical properties using plants and their extracts and poultice together with splint without cast and with the application of systematic massage [51-56]. The use of natural products, majorly plant-based in healing bone fractures is an indicator that phytochemicals may be developed as potential therapy for facilitating the healing process of bone fractures [57]. Nevertheless, how traditional medicine achieves a highly sophisticated kind of postnatal regenerative process that recapitulates many of the ontological events of embryonic skeletal development [58], has ever remained a mystery to many observers!
Ethnopsychotherapy
Also called Traditional Psychotherapy, just like any other types of therapies, aims to mitigate social, developmental, behavioural and emotional difficulties and irrational thoughts, beliefs, cognitions, behaviours and feelings by use and application of the knowledge of the science of behaviour and mind, including conscious and unconscious phenomena, as well as feeling and thought to interact with another affected person to help change the person and overcome problems in desired ways [59,60]. The rationale of undergoing psychotherapeutic processes is to continuously improve an individual’s well-being and mental health, to resolve or mitigate troublesome behaviours, beliefs, compulsions, thoughts, or emotions and to improve relationships and social skills of the affected person in the society while integrating mind, heart, body and spirit but not spirituality unless it is regarding bereavement [61-64]. Certain psychotherapies are considered evidence-based for treating some diagnosed mental disorders in life of certain individuals [64-68].
Phytonutrients
(also called phytochemicals, originates from the Greek word, “phyton” meaning “plant” and from the Latin word, “nūtrīre” meaning “nourish”. These are substances found in certain plants, which are believed to protect plants from damaging environment in which the plants exist and are beneficial to human health and help prevent various diseases. Plant foods contain thousands of natural chemicals, which help protect plants from germs, fungi, bugs, and other threats. Phytonutrients aren’t essential for keeping one alive, unlike the vitamins and minerals that plant foods contain. But when you eat or drink phytonutrients, they may help prevent various diseases and keep your body working properly. Examples include carotenoids, ellagic acid, sulphides, thiols, flavonoids, Proanthocyanidins, flavan-3-ols, resveratrol, phytosterols glucosinolates, anthocyanins, lycopene, phytoestrogens etc., obtained from foods such as fruits, vegetables, herbs and spices including whole grains, nuts, beans, tomatoes, tea, tofu, dates, peppermint, cloves, berries, olives, nutrition-rich onions, leeks, grape juice extracts, cranberries, cocoa, strawberries, nutrition-loaded tomatoes, carrots, sweet potatoes, coffee, garlic, beets, black pepper, cardamom, blueberries, broccoli, asparagus, dandelion tea, earl grey tea, wine, chai tea and green tea are examples of foods high in phytonutrients. Phytonutrients are the basis for more than 40 percent of medications today and have become a great resource of treatment of a wide range of diseases such as pulmonary and cardiovascular diseases, diabetes, obesity and cancer. Phytonutrients also provide colour, flavour and smell to plants. Medicinal plants that are high in phytonutrients include: aloe vera, arnica, arrowroot, milk thistle, clove, dandelion, ginkgo biloba, ginseng, lavender, peppermint, St. John’s wort, Witch Hazel etc.
Nutraceuticals
Are products derived from food sources that are purported to provide extra health benefits, in addition to the basic nutritional value found in foods. Nutraceutical products have been found to prevent chronic diseases, improve health, delay the aging process, increase life expectance and support the structure or function of the body.
Traditional Birth Attendants (TBA)
Also known as a traditional midwife, community midwife or lay midwife, is a pregnancy and childbirth care provider who assists a woman during labour and delivery with skills learned by apprenticeship or personal experience rather than by formal training [69]. Traditional birth attendants provide the majority of primary maternity healthcare in many developing countries and may function within specific communities in developed countries [70,71]. Traditional midwives provide basic healthcare, support and advice during and after pregnancy and childbirth, based primarily on experience and knowledge acquired informally through the traditions and practices of the communities where they originated [72-74]. Many traditional midwives are also herbalists, or other traditional healers in their own unique ways. They may or may not be integrated in the formal healthcare system and sometimes serve as a bridge between the community and the formal health system and may accompany women to health facilities for delivery [69,75].
Anthroposophic medicine (massage, exercises, counselling and substances from plants, animals, soils, water, minerals, microorganisms etc.)
Anthroposophic medicine (or anthroposophical medicine) is a form of alternative medicine. Devised in the 1920s by Rudolf Steiner (1861-1925) in conjunction with Ita Wegman (1876- 1943), anthroposophical medicine is based on occult notions and draws on Steiner’s spiritual philosophy, which he called anthroposophy. Practitioners employ a variety of treatment techniques based upon anthroposophic precepts, including massage, exercise, counselling, and substances [76-78].
Naturopathy or naturopathic medicine
Is a form of alternative medicine that employs an array of pseudoscientific practices branded as “natural”, “non-invasive”, and as promoting “self-healing”. The ideology and methods of naturopathy are based on vitalism and folk medicine, rather than evidence-based conventional medicine. A Naturopath is a health practitioner who applies natural therapies. Her/his spectrum comprises far more than fasting, nutrition, water, and exercise; it includes approved natural healing practices such as Homeopathy, Acupuncture, and Herbal Medicine, as well as the use of modern methods like Bio-Resonance
Homeopathy
Is a system of alternative medicine created in 1796 by Samuel Hahnemann, based on his doctrine of like cures like (similia similibus curentur), a claim that a substance that causes the symptoms of a disease in healthy people would cure similar symptoms in sick people? Homeopathy, or Homeopathic Medicine, is the practice of medicine that embraces a holistic, natural approach to the treatment of the sick. Homeopathy is holistic because it treats the person as a whole, rather than focusing on a diseased part or a labelled sickness.
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Milestones in Ethnomedicines Industry in Kenya:Legislative and Regulatory Framework
It is not exactly known with certainty when humans started practicing ethnomedicines but documentations indicate early records began some 4000 years ago [79] and it may be as old as human history on the Planet Earth. Nevertheless, since pre-historic times, ethnomedicine industry within the various ethnoknowledge systems and ethnic-based cultural settings provided the only sources of healthcare systems worldwide, Kenya included [48,80- 82]. Therefore, since antiquity, this form of healthcare system was well established and unequivocally trusted to take care of any nature of ill-health in human environments [83-86], as its method of discovery must have presented a lot of challenges to these early humans who turned to be guinea pigs in the “laboratory” (in this case, bushes and/or homesteads) for testing. Risks involved consuming poisonous material that resulted into physical damage (both internal and external), chronic diseases, vomiting, diarrhea, coma and even death [20]. By this strategy, the early humans dis covered a variety of food stuffs from plants as well as a wide range of natural medicines [87]. Following scientific evolution and revolution, particularly from 15th Century provided an evidence-based standard of medication and the entire healthcare systems that gave birth to conventional medicine that spread around the world through missionaries, colonialism, slave trade and recruitment to participate into world wars took place [88]. Unfortunately, the spread of conventional medicine occurred at the expense of ethnomedicine industry, which was in return, severely condemned. In reality therefore, this conventional medicine should be the Alternative medicine and vice versa. For instance, in Kenya, the Colonial 1910 Medical Practitioners and Dentists Ordinance therefore did not recognize ethnomedicine industry as a result of its international condemnation and wholesome dismissal and henceforth, total rejection. Rejection of ethnomedicine industry continued post-colonial era with the succeeding African governments, for instance in Kenya, ethnopractitioners were continually condemned in 1969 by former President, the late Mzee Jomo Kenyatta, who did not want to embrace the practice of ethnomedicines at any level, albeit being brought up in such an environment under the care of ethnopractitioners. This was a case of the influence of the western powers on succeeding African governments, a manifestation of the importation of the European law (English Common Law) into Africa that experienced conflict between the foreign law and the indigenous African customary rules, norms, taboos, culture and heritage as the case of Kenya’s Witchcraft Act (Cap. 67) that came into force on 12th November, 1925, Uganda’s Witchcraft Act (Cap. 108) that came into force on 28th March, 1976 and the Tanganyika’s Witchcraft Ordinance (Cap. 18) that came into force on 28th December, 1928 [89]. Noted that these laws were completely rejected as they failed to take into account the customary rules, heritage and socio-economic ethos of the people of East Africa that they were supposed to regulate, notwithstanding the existence of other institutions for their enforcement. It is important to note that these laws were introduced to condemn the practice of ethnomedicines as was equated to witchcraft by the then colonial masters throughout the entire of East Africa region. By this kind of intimidation, the ethnopractitioners were practically threatened and put in a position of dilemma where they could not be able to provide culturally competent and appropriate healthcare services to their clients [90]. Following this reaction, many ethnopractitioners abandoned their work at the expense of Christianity, Islamic and other religious faith and being loyal to the then ruling political regime, a situation that led to the underdevelopment of the entire industry of ethnomedicines.
A. However, the state of affairs of the use and application of ethnomedicines drastically changed when World Health Organization (WHO) realized the value and importance of ethnomedicines in healthcare systems, particularly in Primary Healthcare System of the developing countries [1] Following this realization, ethnomedicines was incorporated into Kenya’s national health policy framework in the late 1970s following the Alma-Ata Declaration, adopted by WHO, UNICEF in 1978 [1], from where it was resolved that traditional medicine had to be incorporated in the healthcare systems of developing countries if the objective of the “Health for All by the Year 2000” was to be realized. “Although traditional medicine has been used for thousands of years and has made great contributions to human health, the Alma-Ata Declaration was the first recognition of the role of traditional medicine and its ethnopractitioners in primary healthcare by WHO and its Member States and Governments”, noted by WHO secretariat. However, African countries did not seriously consider this declaration as important as WHO itself. Nevertheless, Member States and Governments and WHO governing bodies, Kenya included, have adopted a number of resolutions and declarations on traditional medicines ever since the Alma-Atta Declaration of 1978 [1] and have differently impacted on member states and governments include the following noted milestones with special reference to World Health Organization where possible: a) In 1984, a Traditional Medicines and Drugs Research Centre (TMDRC) was established as part of the Kenya Medical Research Institute (KEMRI) to help validate the use and practice of ethnomedicines. For the last 24 years, TMDRC focused on research and development (R&D) without special attention to capacity building component at all levels and embracing the continuum of togetherness for specifically, strategic promotion of the sustainable use and practice of ethnomedicines amongst local and native communities. b) Kenya’s Development Plan 1989-1993 (34) recognized ethnomedicines and made a commitment to promote the welfare of ethnopractitioners who were condemned in 1969 by the then, President, the late Mzee Jomo Kenyatta. c) In 1999, Kenya’s Patent Law was revised to include protection for traditional medicines and its practices. d) Following WHO affirmative action, the Member States and Governments of the WHO African Region adopted a resolution in Ouagadougou, Burkina Faso, in August 31st, 2000 called “Promoting the role of traditional medicine in healthcare systems: A strategy for the African Region Committee.” This strategy provided for the institutionalization of traditional medicine in healthcare systems of the member states and governments of the WHO African Region. e) In Lusaka in July 2001, the Organization of the African Unity (OAU) Heads of State and Government declared the period 2001 - 2010 as the African Decade on African Traditional Medicine. f) In May 2002, World Health Assembly (WHA) launched the first ever WHO Strategy on Traditional Medicine 2002-2005 to help guide states and governments in developing legal framework that can help in integrating traditional medicine into primary healthcare systems. g) In May 2003, WHA made resolutions to promote traditional medicine, including WHA56.31.
h) In July 2003 in Maputo, the African Summit of Heads of State and Government was held and endorsed the plan of action for implementation of the Decade of African Traditional Medicine declared in Lusaka in July 2001 and the institution of the African Traditional Medicine Day in Member States to be celebrated every year on 31st August with effect from 2003. i) In 2005, National policy on Traditional Medicine and Medicinal Plants was drafted and emphasized the need to take inventory of all the medicinal plants in the country. It also sought to encourage the setting up of nurseries and herb gardens with the ultimate goal of bio-conservation and research. Unfortunately, these recommendations are yet to be passed into law by the Kenyan parliament [91]. j) In May 2008, the National Coordinating Agency for Population and Development (NCAPD), a semi-autonomous Kenya government agency that promotes and coordinates population and development activities in Kenya, developed a policy brief No. 1, Seeking Solutions for Traditional Herbal Medicine: Kenya Develops a National Policy. This policy brief summarized the major issues requiring consideration as Kenya worked to develop a national policy for guiding, promoting and regulating traditional medicine in the country. k) On 26th June, 2008 African Ministers of health and heads of delegation of African countries met in Algiers for the Ministerial Conference on Research for Health in the African Region and adopted The Algiers Declaration in which they agreed to promote research in traditional medicine and strengthen health systems while taking into account the socio-cultural and environmental situation in which the people of Africa live. l) In November, 2008, the first WHO Congress on Traditional Medicine, 7-9 November 2008, Beijing, People’s Republic of China was organized by WHO to further assess the role of Traditional Medicine/Complementary/Alternative Medicine (M/C/ AM), to review the progress of countries and to help Member States integrate TM/C/AM into their national healthcare systems. m) On 8th November, 2008, The Beijing Declaration was adopted to promote the safe and effective use of traditional medicine, and to call on WHO Member States and other stakeholders to take steps to integrate TM/C/AM into national health systems. n) In 2009, WHA resolution on Traditional Medicine was adopted on behalf of member states and governments (WHA62.13, 2009). o) In July 2009, The National Policy on Traditional Knowledge, Genetic Resources and Traditional Cultural Expressions, was drafted in Kenya in response to a growing need to address three main challenges facing the country: accelerating technological development, integration of the world economic, ecological, cultural, trading and information systems and the growing relevance of intellectual property rights to these areas of activity. The policy further sought to protect the owners of indigenous knowledge from being exploited through biopiracy and how accruing benefits could be shared amongst stakeholders involved. p) In 2011, World Bank survey estimated that there were potential 40,000 herbalists in Kenya compared to about 4, 500 conventional doctors. This presented and further revealed a very important human resources (health personnel) already existing in Kenya and whose shortage remains the biggest problem facing the national healthcare sector today as was noted by Dr Alfred Karagu, the then acting Chief Executive Officer at the Ministry’s National Cancer Institute (NCI). q) In 2014, WHO Traditional medicine strategy 2002-2005 was updated as WHO Traditional Medicine Strategy 2014-2023 to help healthcare leaders to develop solutions that contribute to a broader vision of improved health and patient autonomy. The strategy has two key goals: to support Member States (Kenya included) in harnessing the potential contribution of Traditional Medicine/Complementary/Alternative Medicine (TM/C/AM) to health, wellness and people-centred healthcare and to promote the safe and effective use of TM/C/AM through the regulation of products, practices and practitioners. r) Witchcraft Act (Cap. 67) that came into force on 12th November, 1925, Industrial Property Act No. 3 of 2001, The Copyright Act, 2001 (Chapter 130, 2014 Revised Edition), The Protection of Traditional Knowledge and Cultural Expressions Act 2016, and The Health Act 2017 ushered in the use and application of ethnomedicines alongside conventional medicine in Kenya. This Health Act of Kenyan Parliament for the first time legally brings Traditional Medicine/Complementary/Alternative Medicine (TM/C/AM) and ethnopractices fully under the national Ministry of Health alongside conventional medicine. While the bending private Bill at the National Assembly (Kenyan National Parliament), The Traditional Health Practitioners Bill, 20l4, sponsored by Member of Parliament, Rachel Nyamai and another similar Bill, The Traditional Herbal Medicine and Medicinal Plants Bill, 2014, sponsored by the Ministry of Health, once debated and passed, will contribute a great deal to the use, control, management and practice of ethnomedicines in Kenya. It therefore follows with logical necessity that in Kenya, the legal framework of TM/C/AM is gradually taking shape in time and space albeit its state of confusion/competition and resistance from the conventional practitioners. s) In 2017, the 2017-2022 Cancer Strategy launched indicated to educate its health workers and communities on the use and application of ethnomedicines for cancer treatment, control and management. t) These major developments from 2014 through 2015 and 2016 to 2017 culminated in efforts suggested way back in 2002 by the then Minister for Health, Prof. Sam Ongeri; to incorporate ethnomedicines at all levels of Kenya’s healthcare system in order to achieve “Health for All” but this was then strongly opposed by conventional doctors in Kenya, particularly the Kenya Medical Association.
u) In February 2017, during the 7th Kenya Medical Research Institute (KEMRI) Annual Scientific and Health Conference, evidence was presented supporting the use of some more than 20 locally available herbs in treating cancer. v) On Thursday, November 23rd, 2017 Maasai Mara University launched the tree planting exercise of medicinal plant species in its newly established, African Medicinal Botanical Garden (AMBG) at its Main Campus. The AMBG is to serve as one of the main ex-situ and in-situ conservation site in Kenya, as a resource centre for medicinal plants for study, research, consultancy and production. Additionally, this is part of the government’s strategy to combat desertification and increase the county’s forest cover and push it to 10 per cent by 2020. w) On Friday, December 1st, 2017, Maasai Mara University in partnership with the Wuhan Botanical Garden (WBG) of the Chinese Academy of Sciences (CAS) and in collaboration with Sino- Africa Joint Research Centre (SAJOREC), invited His Excellence, the Deputy President of the Republic of Kenya, William Samoei Ruto to commission the established African Medicinal Botanical Garden (AMBG). x) On 15th February, 2018, during the 8th KEMRI Annual Scientific and Health Conference with the Theme: Health Research for sustainable Development held between 14th and 16th February, 2018 at Safari Park Hotel, Nairobi, Kenya, KEMRI’s Traditional Medicines and Drugs Research Centre (TMDRC) organized a special symposium to address the state and way forward of Traditional Medicine/Complementary/Alternative Medicine (TM/C/AM) in Kenya.
y) On 31st August, 2018 and following the endorsement of the plan of action for implementation of the Decade of African Traditional Medicine declared in Lusaka in July 2001 and the institution of the African Traditional Medicine Day in Member States to be celebrated every year on 31st August with effect from 2003, Maasai Mara University organized for the National celebrations of the African Traditional Medicine Day.
z) On Wednesday, May 22nd, 2019 Maasai Mara University launched the Kenya Defence Forces (KDF) tree planting exercise of mainly medicinal plant species in its newly established, African Medicinal Botanical Garden (AMBG) at its Main Campus. The AMBG is to serve as one of the main ex-situ and in-situ conservation site in Kenya, as a resource centre for medicinal plants for study, research, consultancy and production. Additionally, this is part of the government’s strategy to combat desertification and increase the county’s forest cover and push it to 10 per cent by 2020.
As much as the three Acts mentioned in i) above need to be put into practice as the debate at the National Assembly awaits The Traditional Health Practitioners Bill, 20l4, and The Traditional Herbal Medicine and Medicinal Plants Bill, 2014, care need to be exercised in order to avoid contradictions and duplication of functions, operations, powers and authority of the various Acts involved in fostering appropriate integration, regulation and supervision at all levels, particularly the regulation of the capacity building, research, products and practice of ethnomedicines as well as ethnopractitioners
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Great Challenges with Ethnomedicines: Public Health Concerns and Required Concerted Efforts
The use of modern science and/or conventional knowledge to measure ethnomedicines as an independent and unique system and two, the failure of conventional medical practitioners to fully recognize and accept ethnomedicines in the healthcare system, pose great challenges on how to alleviate the mistrust that has endured for ages. Restoration of confidence in an ethnomedicinal system labelled as satanic, witchcraft, myth, quack, evil, apprehensive etc, requires concerted efforts at all levels, providing evidence-based knowledge in public domain. Growing use and application of ethnomedicines and their market expansion pose challenges on safety, quality and efficacy of traditional remedies, notably assessment of products and services, qualification of ethnopractitioners, methodology and criteria for evaluating efficacy, which is unique to Traditional and Complementary Medicine (T&CM) and ethnopractitioners. Contaminated an adulterated products are common in ethnomedicine industry and reports of T&CM–based toxicities are also alarming in public [92]. Continued use and application of endangered species such as Slow loris and Shark fins in ethnomedicines without precautions and proper guidelines endanger and threaten the long-term survival of ethnomedicine industry. There is need for the development of the ethnopharmaceutical production policy and regulations governing chain supply strategies of the ethnomedicine industry. This has to be supported by the political will to provide the enabling environment for working and implementation of the Acts. The effect of poisonous ethnomedical resources and use of human as the guinea pig in the “laboratory” is really a worrying factor! The maintenance of a working relationship between ethnopractitioners, conventional practitioners and the industry/academia is indeed challenging. Similarly, the sustainable integration of T&CM into conventional healthcare system and scaling to cover primary, communal and national levels is just an uphill task, in particular identifying and evaluating strategies and criteria for integrating T&CM into national health and primary healthcare (PHC) systems. Lack of development of national policies to guide the sustainable utilization, protection, conservation and preservation of ethnomedical resources: plants, animals, soils and microorganisms versus nature conservation. Limited national organizational arrangements for institutionalization of Traditional and Complementary Medicine. Lack of insurance cover of those patients seeking health services of T&CM. Capacity building in Traditional and Complementary Medicine: what levels and how should the curriculum be developed, controlled and regulated, and by who? In Kenya, the law under development is however silent on this particular aspect, thus presenting a dilemma and creating loopholes that may be used unprofessionally. There is need to develop concerted efforts to address the issues of Intellectual Property Rights (IPR) of ethnoknowledge systems, equitable sharing of accruing benefits following commercialization of T&CM, bioprospecting and biopiracy at local, national and international levels. There is also need to ensure the availability of relevant information, enhancing access and promoting rational and international use and application of Traditional and Complementary Medicine
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Which Way Forward for Ethnomedicines? The Recognition of Ethnopractitioners And Continuum of Togetherness.
Dreaming big to improve livelihoods in the society as we all read from the same script without mischief! Recognizing the fact that ethnopractitioners, conventional practitioners and industry, research institutions and academia are all equal partners, collaborators and/or players in health industry in Kenya. As a matter of facts, the three partners/collaborators/players must agree that globally, they are co-existing, competing, communicating, coordinating, collaborating and cooperating in their own unique ways of embracing survivalihood and henceforth, continued evolution of life that is optimally taken care of. Upon this realization, the three partners/collaborators/players should therefore come together to develop concrete, attainable goals and objectives with shared vision and the unique purpose of co-existence. The relationships for engagement should focus on a 3C framework of embracing coordinative, collaborative and cooperative in order to realize the unique purpose of co-existence in the health industry. The collaborative arm will ensure that the three partners are all equally involved in a plan or activity work together in an organized way for effective results and mutual benefit, authority and accountability for success and sharing of resources and rewards. The cooperative arm will ensure working jointly with others or together especially in an intellectual endeavor for effective results. While the coordinative arm will ensure that the three partners are working together to create or achieve the same thing. All these levels of engagements will improve the weaknesses of the three partners a great deal while building their strengths to greater heights as they grow and develop a healthy society [93-96].
I. The suggested continuum of togetherness should be able to: 1. Develop a robust and sustainable strategic mechanism of sensitization of the ethnomedicines at all levels in the local and native communities. 2. Provide a harmonious working relationship between ethnopractitioners and conventional practitioners with a view to institutionalizing traditional medicines in healthcare systems, jointly developing sustainable policies and products for implementation and unbiased regulatory mechanisms. 3. Initiate ethnomedicines research and development agenda involving ethnopractitioners, conventional practitioners and industry, research institutions, industry and academia to help provide scientific evidence on safety, efficacy and quality of ethnomedicines where necessary as observed during the Ministerial Conference on Research for Health in the African Region, held in Algiers, Algeria, from 23rd to 26th June, 2008 and adopted as the Algiers Declaration, 2008. 4. Initiate a regulated and controlled capacity building framework at all levels, including refresher courses for leading ethnopractitioners, Professional Certificate, Diploma, Undergraduate Degree programmes, master’s degree and Doctoral Degrees. However, integrate WHO-based training tools in traditional medicines and Primary Healthcare (PHC) to the training programmes, syllabi and curricula.
5. Renew the lost glory in traditional medicines by recognizing ethnopractioners as equal partners through a well-structured institutional-based policy document on Intellectual Property Rights (IPR) to protect the interest of the indigenous communities and its ethnopractitioners and taking into account fair and equitable sharing of benefits of relevant holders, in collaboration with relevant partners with regard to WIPO requirements in Geneva and as per the multilateral treaty, Convention on Biological Diversity (CBD) signed by 150 government leaders at the 1992 Rio Earth Summit. The Convention on Biological Diversity is an international legally binding treaty with three main goals: conservation of biodiversity; sustainable use of biodiversity; fair and equitable sharing of the benefits arising from the use of genetic resources. The WHO guidelines and regulatory frameworks for the protection of traditional medical knowledge and access to biological resources to their specific situations can therefore be guaranteed.
6. Initiate the formation of a regulatory National Board for Traditional Medicines, which will regulate and control the practice of traditional medicines, ethnomedical resources and capacity building at all levels including, refresher workshop- based training programmes for leading ethnopractitioners, Professional Certificates, Diploma, Undergraduate Degrees, master’s degrees and Doctoral Degrees. Will focus on capacity building skills in problem solving, communication, synthesis of information and working ability. 7. Promote collaboration and partnership amongst the three partners and play a key role in allocating and mobilizing adequate resources and strengthening capacity-building. 8. Harmonize the relevant sections of The Protection of Traditional Knowledge and Cultural Expressions Act, 2016 and Health Act 2017 together with that of the bending Bills at the Kenyan National Parliament, The Traditional Health Practitioners Bill, 20l4 and The Traditional Herbal Medicine and Medicinal Plants Bill, 2014, into one formidable Act that addresses all aspects surrounding ethnomedicine industry in Kenya.
9. An all-inclusive national taskforce with clear terms of reference need to be set up, with leadership preferably from the academia (because of its robust constitutional mandates on capacity building, research, community outreach and consultancy) so that a concept is drafted for discussion with the parliamentary committee in-charge of health in preparation for the development of a Bill to be tabled on the floor of the Parliament for debate and subsequent development into Act. 10. Promote interdisciplinary systems such as Traditional and Complementary Medicine to work in integrated approach to generate solutions to complex, dynamic societal problems, manage adoptively and inform decision-making processes. 11. Enhance resource mobilization, wealth creation and improvement of the livelihoods of marginalized communities identified to be the custodians of the indigenous knowledge, Traditional and Complementary Medicine and ethnopractices faced with either exploitation and/or extinction.
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Anticipated Benefits Accruing from the Envisioned Continuum of Togetherness
Recognizing ethnomedicines as having a wide variety of therapies and practices and as one of the resources of primary healthcare services familiar to many people in the world, its growth and development through partnership and collaboration of ethnopractitioners, conventional practitioners and industry, research institutions and academia as equal partners in the health industry will result into a lot of benefits as follows:
i. Improved health and livelihoods in the society due to increased availability, accessibility and affordability of healthcare services. ii. Quality training/teaching of students, this is good for society. iii. Increase in business and profit-making for involved partners. iv. Increase in amount of research for partner institutions. v. Sustainable utilization of natural resources and conservation strategies. vi. Improved health industry at all levels. vii. Increased employment opportunities. viii. Ranking of institutions involved improves nationally and internationally. ix. The interaction makes staff improve in competencies. x. Increase in research output in form of products, publications and patents etc. xi. Pooling resources and individual strength together for a common goal xii. Increased and improved commercialization of knowledge xiii. Joint sourcing of research funds: easy to get! xiv. Sharing of research facilities and rewards.
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The Case of Maasai Mara University in Narok County,Kenya
Maasai Mara University has partnered with The Wuhan Botanical Garden (WBG) of the Chinese Academy of Sciences (CAS), and in collaboration with Sino-Africa Joint Research Centre (SAJOREC) to establish an African Medicinal Botanical Garden (AMBG) at the Main Campus of the University. This idea of collaboration of a Chinese- aided China-Africa Joint Research Centre in Kenya, where African and Chinese researchers would conduct joint research, was conceived during deliberations between Prof. Robert W. Gituru of Jomo Kenyatta University of Agriculture and Technology (JKUAT) and his Chinese research counterpart, Prof. Wang Qing Feng, in 1999. Ever since its establishment, the Sino-Kenya ties has yielded a lot of fruits, among them, the ‘Sino-Kenyan Scientists’ Workshops’, the JKUAT Botanical Garden, training sessions for African stakeholders to various Universities and research institutes in China, scholarships for Kenyan students to the prestigious University of Chinese Academy of Sciences, and the on-going work of African Medicinal Botanical Garden of Maasai Mara University, Narok County.
The University is developing the African Medicinal Botanical Garden on a 20-acre land to support teaching, consultancy, community outreach services, research and product development in the areas of Traditional Medicine/Complementary/Alternative Medicine (TM/C/AM) (Figure 1). The AMBG will serve as one of the main ex-situ conservation sites in Kenya, as an ethnobotanical and ethnomedicine resource centre for study, research, product development and consultancy, and as a centre for information dissemination on ethnobotanicals, ethnomedicines as well as general ethnoknowledge. The project commenced by planting a few available medicinal plant species (1053 seedlings comprising 67 species) from the nurseries of trees of Kenya Forestry Research Institute (KEFRI) headquarters and elsewhere in its branches during any rain seasons. Additional planting took place with medicinal plant species, which were raised in a plant nursery established at the University’s Main Campus, Narok County following capacity building of the University’s staff.
Careful observation of Figure 1 indicates that the area is a semi-arid one, therefore implying that the area has limited amount of water supply and/or availability. The University therefore innovatively devised a technology of cleaning wastewater using a biological means, which is Common Water Hyacinth plants filtering system. Semi-cleaned wastewater from the sewage is pumped into a series of joined compartments, each filled with the Common Water Hyacinth plants for further purification before it is released in the field for watering plants at various designated water points. Meanwhile, in preparing to ensure that as a stakeholder institution, Traditional and Complementary Medicine work in integrated approach, the University is keen at establishing the state-of-theart laboratories for teaching, research and product developmentIn addition, the University is planning to engage the local communities so as to partner and collaborate with them in research and product development in the areas of Traditional Medicine and capacity building.
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Conclusion
It is important to fast track the organization and integration of ethnomedicines into the mainstream conventional healthcare systems at all levels and in line with the WHO guidelines and standards as outlined in its resolutions, declarations and strategies within the framework of the country’s constitution. For sustainable growth and development of Traditional Medicine/Complementary/ Alternative Medicine in Kenya, the two Acts and the two Bills yet to be tabled in the National Assembly, there is need to be harmonized them into one formidable Act through a national task force, preferably led by the academia for its strong constitutional mandates on capacity building, research, consultancy, community outreach services and policy formulation. In this Act, clarity on control and regulation of capacity building, research, practice, Intellectual Property Rights (IPRs), well defined functions, powers and authority of the Council/Board, mandates of operating committees and sub-committees, and consultation and inclusivity amongst concerned stakeholders should not be ignored. It should be noted further that IPRs are protected in law by, for example, patents, industrial designs, copyright, geographical indications and trademarks to help foster a culture in which innovation and creativity are celebrated, protected and enable people to earn recognition or financial benefit from what they invent or create.
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Acknowledgements
We are very grateful to the Wuhan Botanical Garden (WBG) of the Chinese Academy of Sciences (CAS), and Sino-Africa Joint Research Centre (SAJOREC) to partner and collaborate in establishing an African Medicinal Botanical Garden (AMBG) at the Main Campus of Maasai Mara University. We are also thankful to the organizers of the 8th KEMRI Annual Scientific and Health Conference with the Theme: Health Research for sustainable Development held between 14th and 16th February, 2018 at Safari Park Hotel, Nairobi, Kenya, where this manuscript was first presented and critiqued by colleagues during the KEMRI’s Traditional Medicines and Drugs Research Centre (TMDRC) organized symposium to address the state and way forward of Traditional Medicine/Complementary/Alternative Medicine (TM/C/AM) in Kenya. To them all we are very grateful.
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Lovely by the Water- Reflections on the Pleasures and Benefits of Doing Qigong in Natural Surroundings
Qigong and Nature are intertwined. The image of water is omnipresent in Taoist philosophy as Taoist sages believe we are always immersed in Tao that our lives are spent in this moving river of life. Images of trees usually willows situated near water their branches moving in the gentle breeze permeate the ancient texts which guide Qigong practice. While there has been considerable research recently on Green and Blue Spaces on exercise in general however, there has been very little modern research on the health or any other benefits of practicing Qigong outside in natural surroundings. This article offers a commentary on the experience and perceived benefits of practicing Qigong in natural surroundings. It presents 20 years of teaching Qigong classes in Natural surroundings through the eyes of my students and of Qigong colleagues around the world and places these in the context of academic research. It concludes that the ancient Taoist and Buddhist master’s perceptions written many years ago are correct. The quality of the experience health benefits, and the energy accrued from Qigong is enhanced by practice in nature.
Keywords: Qigong; Health; Wellbeing; Nature; Water; Forests; Green space; Blue space;
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Introduction
Context and limits
I retired from full-time University teaching 3 years ago. One day I sat down and realized I have been offering Qigong classes for over 25 years and outside in natural surroundings for more than 20 years. As I started to reflect on this. I decided to write an article about this topic. My intent was to gather personal anecdotes about my own experience and the experiences of my students and Qigong colleagues around the world about their perceived benefits of practicing Qigong in natural surroundings (by/over water amongst trees in fields on hills/mountains etc.) and to put these in context against a backdrop of academic research drawn from international studies on health benefits of Qigong and of being in Nature. I sent a brief note to my current and former students and to a few other colleagues around the world. I asked each of them:
“are you able and willing to contribute some personal anecdotes-a few lines (or more if you wish) about your own previous experiences practicing Qigong in natural surroundings. This could be about what you felt or saw and/or about any perceived benefits you experienced”. Their comments are recorded verbatim indented and in italics in the text. However, what follows is not a research paper per se but rather focusses on personal reflections on my own journey through both Qigong and research and the experience of others who engage in Qigong practice within nature Foot note.
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The Road to Qigong for Healthy Adults
I have practiced Japanese and Chinese Martial Arts for over 50 years. In 1993 I met my current teacher Master George Ling Hu [1]. In 1994, Master Hu encouraged me to offer Qigong and Tai Chi classes for adults. I began very slowly but offered both Tai Chi and Qigong. I quickly realised that while Tai Chi was more recognisable and thus more popular it was also more difficult for participants especially those who attended classes only sporadically. Participants who attended only 3 of 10 classes and those who did not practice forms outside of class time were not only frustrated but also frustrating to participants who attended regularly and practiced dutifully outside of our class meetings. Qigong on the other hand was easier for students to pick up and attend classes as their schedule allowed. Also, I was drawn more and more to its benefits for health. In part this is because it is easier to begin and practice as there is less to memorise but also because in my opinion the benefits to participants were more tangible.
In the past 25 years I have offered regular weekly Qigong classes for adults in many diverse locations including among other spaces: a small pharmacy a large university gym a portable ‘Nissan Hut’ various Yoga studios and even a realtor’s office in various communities throughout Ontario Canada. In addition to my regular weekly classes I have run weekend classes and Train the Trainer workshops and programs based on these weekly classes at various locations worldwide including Australia, China, Portugal, New Zealand, South Africa & the UK
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Qigong in Natural Surroundings: Research as Context
While the term “Qigong” is a modern construct many of the methods that are used today are derived from age-old Chinese traditions-most notably Taoist & Buddhist longevity (so called immortality) techniques, meditations and martial arts training exercises [2]. The monasteries of Wudang (Taoist) and Shaolin (Buddhist) feature prominently in the history and practice of what has come to be known as Qigong. Wudang Temple was situated high in the Wudang mountains and Shaolin Temple high in Song Mountains. Each in their own way exemplified and in modern times inform the idea of practicing Qigong outside in Nature. In researching this article, I was initially struck by the paucity of research connecting natural surroundings and Qigong. True there are references in several ancient texts to the value of practicing amongst trees or on mountain or by lakes and there are some modern articles primarily in health blogs that follow the same line. However, I found almost no research qualitative or quantitative specifically linking the health or any other benefits of practicing Qigong outside in natural surroundings. However, the therapeutic value to Human Health and Quality of Life of “Green Spaces” (i.e. woods and natural meadows, wetlands as well as urban parks and sports fields) and “Blue Spaces” (i.e. waterfront parks, harbors, ports, marinas, rivers, open air streams, canals, lakes, ponds and fountains) and especially their inclusion within urban environments is well documented [3-7]. Research in this area is extensive and ranges from studying the effects of simply spending 5 minutes in an urban park to the practice of Shinrin-yoku (bathing in the forest atmosphere or taking in the forest through our senses) – usually referred to simply as Forest Bathing. Dr Qing Li suggests Forest Bathing can reduce your stress levels and blood pressure strengthen your immune and cardiovascular systems boost your energy, mood, creativity, and concentration even help you lose weight and live longer [3]. Other findings from research on exercising in Green and Blue spaces which are often interlinked include the following:
I. Participants who exercised outdoors expressed more restorative feelings compared to ones who exercised indoors … (they) said they felt like the activity allowed for them to escape they were fascinated by their surroundings they felt like their interest during the activity was sustained [4]. II. Compared with exercising indoors exercising in natural environments was associated with greater feelings of revitalization and positive engagement, decreases in tension, confusion, anger, and depression, and increased energy [5]. III. A positive association between greater exposure to outdoor blue spaces (with) benefits to mental health and well-being [6].
The overwhelming finding from research on exercising in Green and/or Blue Spaces is that each have significant health benefits for human beings. The term Green Exercise has been coined in the research to describe any activity in the presence of nature irrespective of whether this is deemed a Green Space or a Blue Space. The evidence shows that pursuing Green Exercise leads to positive short and long-term health outcomes. Getting outside and moving for as little as five minutes at a time improves general good health. Every green environment improves both self-esteem and mood; the presence of water generated greater effects. Interestingly, low-intensity to moderate-intensity physical activity shows greater improvements in self-esteem than high-intensity outdoor exercise [7].
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Research on The Effects of Nature on Health and Well-Being
Qigong in nature is the ultimate gift: The tangible nurture which nature brings me plus the renewed energy I feel being bare foot on the earth-is like an enhanced power boost to my body, mind and spirit-all in one go. Qigong and Nature are intertwined. As mentioned elsewhere [8] images of water are omnipresent in Taoist philosophy, as Taoist sages believe we are always immersed in Tao that our lives are spent in this moving river of life. Images of trees, usually willows, situated near water, their branches moving in the gentle breeze permeate the ancient texts and artwork which guide Qigong practice [9] while Lao Tzu and other Taoist Sages make many references to trees, water and nature in general [10]. The exercise Stand Like A Tree, the basic exercise for Yi Chuan-Zhan Zuang, (the so-called Iron Shirt training exercises for building strong bones and muscle) is based on the Chinese theory-sky above, earth below, and man standing like a tree rooted between the two. Considerable research has been conducted on the benefits of Qigong and remarkable results have been suggested not only for persons with medical conditions (such as High Blood Pressure, Cancer and even Spinal Cord injuries) but also in helping to prevent illness and increase longevity [11]. My own research over the last 20 years has suggested that 30 minutes of seated Qigong helps normalize blood pressure, lower pulse rate and reduce stress. This research also showed that participants reported having more energy, less illness (shorter duration and less severe) and generally felt healthier [1,8]. However, the ancient Qigong masters prescribed how and when to practice. How the time of day, and the direction one faces while practicing influence the effects of Qigong on the body and mind. Similarly, the position of the sun and whether one should have its rays in front or behind you. Emphasis was placed on the four primary times to practice: Sunrise, Midday, Sunset, Midnight, because of the quality of energy at these times. In addition, the Ancient texts warned against practicing in cold wet windy conditions especially with wind behind you as this leached chi from the body.
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Let’s Take this Thing Outside
In 1993 I was still practicing the Japanese (Judo, Ju Jitusu, AiKi Do) and Chinese martial arts forms (Tai Chi, Ba Gua, Kung Fu) I had learned over the previous 25 years from my former teachers such as Sensei O’Tani and Professor Chee Soo. Slowly I was starting to introduce the Chinese forms I was learning from Master Hu into my routine and was practicing for 60- 90mins each time sometimes 2-3 times a day. I found practicing indoors somewhat restricting and so started to move my practice into my back yard and practiced there until the snows made it impossible. My old cat Tikka a Zen master of the first order would accompany me and ‘help’ me in my practice. However, my backyard was not a particularly tranquil space and did not at the time have great Feng Shui. Although later I did put in a fishpond which changed the ambience and distracted Tikka and the other cats. At that time was living in Windsor Ontario Canada and my house bordered a large city park - Jackson Park. One day I decided to move my daily practice to the park. So sometime around 4am I would walk for 5 minutes find a space amongst the trees as far away from houses and the road as I could. I would practice until just after sunrise and then return home and get ready for work. Once in a while this regime was disrupted such as the time the Police stopped and asked me what I was doing-I was practicing Sword forms at the time and they were a little concerned that I was wielding a dangerous weapon in a public park-albeit at 4:30 in the morning!
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Classes on the Water
I first began to offer Qigong classes on the water in 1998 when my then assistant John Taylor was living on the Lake St Clair and offered me the use of his deck to teach. I offered 5 linked classes on his deck which was almost at lake level alongside the water. By any measure this pilot project was very successful. Soon after this I moved to live in a house that had a deck that extended out over Lake Erie. For 12 years I offered classes on the deck 1-2 times a week throughout the summer come rain or shine. In 2015 I moved again and for the past 4 years I have been offering classes once a week on a bluff which looks over Lake Erie and from which point you can see all the way to Ohio. Each of these locations offers a different aspect and feeling but the quality of energy experienced practicing by a lake is special particularly when conducted at a time that we can watch the sun go down.
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Breathe and Smile Qigong
Over the years I have come to call the classes I teach Breathe and Smile Qigong. This came about when I realized that Master Hu was always smiling and sharing jokes and witticisms to help his students relax. I came to realise that this was not an accident but a conscious teaching tool because when you simply breathe and smile you relax making it easier for Chi the essence of Qigong to flow. The Breath and Smile Qigong class I teach has changed only a little over the years. It was designed to run for a total of 1hr with 50-55mins of this being exercise. I have run the class at various times throughout the day such as at lunchtime (12- 1), immediately after work (4:45-5:45). However, for the last 16 years my class has run predominately in the early evening (6:30-7:30). Irrespective of the time offered the most significant classes have always been those I have offered in natural surroundings. Over the last 20+ years the class size has varied from 6-24 people. On average 8-10 people regularly attend class each week. By and large the participants are relatively healthy and come to the classes to remain that way. Most are women aged 45-80 but occasionally my university students’ former students participants’ children and the occasional man (usually a participant’s partner or spouse) also attended. Participants have been very loyal often attending for more than 3 years and usually only stop coming to class due to health problems (their own or family members) or relocation.
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Shape of the Classes
While specific exercises change from time to time the program follows a simple and similar pattern. I use exercises described in Stand Breathe Smile [12] and for many years the sequence of exercises in my classes was: a. Sinews Changing Exercises b. Preparing the Body for Action c. Opening and Closing Breaths d. Ba Duan Jin e. Sinews Changing Exercises – Repeat f. Opening and Closing Breaths – Repeat g. Cool Down
However, as I and the participants in my classes have been growing older some of these exercises have become more difficult to perform without frustration. So more recently I have started to include exercises which are more suited to people as they age. About 2 years ago I started to include more Yi Quan (Standing Mediation or Standing Pole) exercises being careful to limit the length participants hold each posture. Slowly working up from holding each pose 3 long breaths to 2mins. More recently still I have introduced some simple exercises from Wu Qin Xi (Five Animal Frolics). In part I introduced these because the exercises work on specific areas of benefit to aging humans e.g. Crane Stretches to the Heavens (improves breathing) Crane Stands on one Leg (helps improve balance) and Crouching Tiger Grabs Prey (strengthens muscles and bones). I also introduced them because I am working on a health promotion book for younger children linking the Five Animal Frolics health and environmental awareness especially to animals and their habitats
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Commentary
Connections to Nature in General
Many participants made general comments about being surrounded by nature: Practicing in a natural setting near trees and water provides tangible feedback that encourages the natural relationship we as humans have with our environment thus creating a deep sense of peace and well-being.
Specific exercises help connect to nature
Other participants made references to how specific exercises amplified the feelings of being in nature: A. The exercises themselves have an outdoor feel as the fresh air fills my lungs when I stretch my arms wide doing Cleansing Breath. I see the sky above when I raise my arms up to perform the exercises (Separate Heaven and Earth. Salute the Sun or Place the Moon Back in the Sky). My fingers sense the water in the lake when my hands cup and move like Lilies Floating on the Water. B. Being outdoors, Lotus Flower Opening & Lotus Flower Closing are the two exercises where I am able to completely let go relax my mind and be one with it all. The feeling is fleeting but it is there and draws me back for more.
Health and well being
Interestingly few participants wrote about specific health benefits from practice outdoors. Most were on the general nature of feelings of wellbeing and tranquility. a. Qigong exercises in a peaceful tranquil outdoor setting nourish both body and soul. b. I found it easier to connect with my senses so, I felt connected to my body. The sense of somatic connection happened more quickly and felt more profound than when I was indoors. c. (playing) calming music helps calm the mind and body to allow focus on breathing. once this happens, you hear nature’s music. Waves, rustling leaves, birds, bugs and you feel the breeze and tiny ants crawling on your toes. Healing and loving one’s self are made possible. d. However, several did comment specifically on health benefits e.g: Through the movements, I found my physical self, relaxing and healing. Stress, tension and muscle aches gently dissolved. I found that my digestive properties were strengthened, and joints became more supple. With increased flexibility and more energy. I could move on to the next event in my life with greater ease and a clarity of mind a focus that wasn’t there prior to the session.
Meditative/calming effects
Many commented about the meditative and calming nature of being outside i. Performing Qi Gong in natural surroundings gave me a sense of tranquility and peace-each simple repeated movement was much like a repeated mantra in meditation: the focus and repetition allowed my mind to relax allowed my thoughts to let the noise go and a sense of ease and peace ensued. ii. (Outdoor practice) is quieter easier to concentrate and lose yourself in the act good for the mind as well as the body. iii. With Nature’s presence all around. I begin to relax in body, mind & spirit and connect with the feeling energy from the heart. iv. The outside environment has a transformative effect on Qigong exercises making the movements less mechanical and more invigorating; exercises flow more freely and naturally as I feel the gentle breezes off the lake smell the roses in the garden and see the sun peeking through the cloud’s overhead. v. Outdoor practice left me feeling rejuvenated because not only did it allow time to connect with myself but also time to reconnect with natural elements that resonate deep within.
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Teachers Comment on Effect on Their own Students
a) Several other teachers talked about the effects of Qigong on their students: whenever we were forced to move our class indoors due to inclement I found my participants were much more talkative; it took them longer to get settled and harder to remain focused throughout. It’s interesting because there was actually less going on around us while we were practicing indoors. b) Others talked of how difficult it is sometimes for a student to allow nature in: Sometimes (it takes a) long time to get rid of old habits and ways of thinking and being able to see change in one’s own life differently and reconnect with nature where we are a part of! c) At which time guidance can come in the forms of guiding the senses: Feel the wind how he embraces you! Listen to the songs of the birds and leaves of the trees how they sing the song of life for you! Feel the touch of the sunlight which shines through the trees to warm your skin! See the play of colors of the flowers and the butterflies dancing in the wind!
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Biting Insects and Qigong Armour
Practicing outside has its problems from time to time. As one older participant observed: I sometimes found the heat, mosquitos and the time of day challenging. Mosquitos and biting flies are a reality of a South-Western Ontario summer. However, as another participant commented they are not usually a problem while practicing Qigong: I seem to attract mosquitoes and often the bites become very inflamed to the point of leaving me with a bruise. In addition, I avoid the use of insect repellent so I wasn’t sure if practicing Qigong in the evening on a lakeshore would be possible for me to do comfortably. To my great surprise while practicing Qigong. I was not bothered at all by mosquitoes! the way mosquitoes stayed away from me. was so surprising. I remember you described it as “Qigong armour”. This idea of Qigong Armour is very interesting to me. It is commented on obliquely in some of the ancient texts and I have found that when practicing Qigong these biting insects are unable to pierce my skin. I can feel them trying but to no avail. I am not sure if this is a side effect of studying so called Iron Shirt techniques or practicing Qigong in general for so many years
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Changing the Weather: Yi Leads Qi-Qi Moves Clouds?
The Weather is another matter. The reality is the weather is often fickle and beyond the control of the Qigong teacher. Summer in my area is sub-tropical-very hot, humid and we get LOTS of thunderstorms that often come out of nowhere. There have been days when the weather was so inclement that class had to be cancelled. However, in over 20 years of practicing Qigong by the water and amongst trees these days were very few and far between. On the occasions when the weather changed while we were practicing Qigong on my deck over Lake Erie. I tried a technique I had read somewhere in an ancient text where masters gathered the energy of the group and used it to affect the wind and rain. What surprised me was it works! Using the concept of Yi leads Chi I shaped the energy of the group to divert the rain and thunderstorms. It was almost unbelievable-it was thundering and raining ALL around us and yet it was as if we were in a bubble with no lightning or rain! If this had happened once I would have said it was a coincidence but I managed to do this on numerous occasions. Everyone who was present on these occasions spoke of the energy they felt and the experience itself as being beyond belief!
Interactions with wildlife and domestic animals
During my practice I have experienced interactions with domestic animals and wildlife. I have been watched closely by coyotes’ foxes and deer during my practice. Eagles have swooped low over my head to take a closer look at me and I have even been dive bombed by a squadron of purple martins while practicing my sword forms. So persistent was this attack that I had to use the flat side of my sword to repel their attack! Dogs and Cats are naturally drawn to Qigong. My own cats used to join me every morning on my deck over the water. One time in Portugal I was practicing outside among the trees and my host’s cat Tigger sat on a wall. a. As she commented: I will never forget my cat Tigger mesmerised with your slow movements, sitting on the wall watching you for a whole hour and following your slow movements with her head in time and as if hypnotised. b. Another couple commented: We both have extremely fond memories of your cats hanging out and practicing with us! I have often spent large parts of my practice with a cat rubbing itself between my legs or laying down in the exact spot where I have to place my foot.
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Discussion
Being outside whether under the warmth of the sun the heat of the humidity the cool breezes from the offshore wind the sights and sounds of the lake the lovely colours of the sunset and the rising moon with its emerging stars. The essence of being outside in natural surroundings enhances all the senses to contribute to a better quality and more mindful practice! By and large the writings of the ancient sages were poetic written at a time before the internet and google and prior to the ascension of modern science with its emphasis on evidence based best practice. Nevertheless, my own experience and that of many others is that natural surroundings affect Qigong practice. Not only this but each location has its own Feng Shui effect that exerts an influence on the quality of energy felt and thus the overall experience itself.
As one participant put it: In addition to the benefits of improved flexibility and strengthened immune system in a studio type setting there is infinitely more benefit to the practice of qigong in a natural setting. I found that I began to unwind as I began sensing the tranquility of being on the lake. The sunsets were beautiful the fantastic view of the water as we focused upon the horizon all added to managing tension and anxiety. A truly spiritual experience transmuting all the hustle and bustle into sheer peacefulness and bliss.
I have found there is a difference between practising over water, alongside water and simply viewing water. Exactly what this is hard to describe for like attempts to describe Tao the feeling is visceral fleeting-embodied rather than quantifiable or describable. The presence of trees near the water also exerts an effect. The quality the taste of the air in each natural surrounding is different. Practice with warm summer wind is very invigorating. Breathing in as the wind blows creates an amazing feeling of the body filling with energy
One person commented
Performing Qi Gong next to the water was very beneficial for me as water has always held great spiritual meaning for me. I feel a sense of deeper meaning to the universe when surrounded by bodies of water. The trees flowers birds and other aspects of nature also helped create this space of spiritual healing. When performing the Qi Gong i gained a sense of balance in energymy physical being and spiritual being became one. This in many ways summed up my own experiences. For me from the very beginning the feeling of practicing in natural surroundings amongst the trees hearing the bird’s dawn chorus and the quality of the energy experienced felt tangibly different from practising indoors even in the most balanced space with great energy and light. The later classes by the water had different views and birds but the feelings and the energy experienced were very similar. My own experience and those of my students and other teachers around the world tends to reaffirm the ancient Taoist and Buddhist masters perceptions written many years ago that the quality of the experience the energy and the overall health benefits accrued from Qigong is enhanced by practice in nature
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A Contemporary Positional Multi Modal Assessment Approach to Training Monitoring in Elite Professional Soccer
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Understanding positional differences in terms of specific movement, speed and mechanical demands imposed upon elite professional soccer players in training and match play is of paramount importance to further develop player performance. The primary investigation aim was to examine a novel multi-metric method and reveal the representation of a tapered structure referencing a direct relationship with competitive match demands. The study involved 29-elite male European soccer players (26.7±4.07 years, height 183.4±5.87cm, body mass 78.4±8.03kg, VO2max 57.55±5.32mL.kg−1.min−1 and sum of 8 skinfolds 54.12±13.65mm). Daily positional TL data relating to competitive match day (MD) data was assessed via a Global Positioning System (GPS) across a 22-week period during the mid-phase of the competitive season. Results revealed differences between playing positions; central forwards (CF) revealing lower average intensity values when compared with CBs, FBs and WFs (p<0.005). On MD-3, CBs revealed significantly higher intensity scores when compared to CFs and CMs, and WFs with higher intensity scores for CFs. Furthermore, FBs had higher volume session scores than CBs, CFs and CMs on MD (p<0.005). Indications reported in this investigation are novel and vitally important when trying to understand positional TLs in relation to match loads among elite soccer players as well as ensure correct preparation of competitive demands. To conclude, the specific multi-modal approach used allows practitioners to combine key positional mechanical volume and intensity metrics as part of a player positional monitoring strategy and ensure a greater focus on targeted physical stressors. As a result, this may improve the training specificity of positional training workloads in direct relation to match-play, subsequently leading to a greater link between increased performance and reduced injury risks.
Keywords: Soccer; GPS Analysis; Tapering; Periodisation; Positional Training Load
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Introduction
Team sport success depends upon numerous variants such as player skill, fitness, squad size, tactics and psychological factors, as a result, investigations continue to show an ever-growing appreciation and progression for the use of tools to quantify and monitor training loads (TL) of professional soccer players [1,2]. At the elite level the use of global positioning systems (GPS) to report daily TL placed upon on the individual and collective team is becoming more commonplace [2,3]. Although it is widely accepted that the use of GPS can provide a more detailed summary of the TL (Intensity x Duration) [4] GPS literature has generally provided isolated metric outcomes such as total distance covered (TDC), high-speed running (HSR), sprint, acceleration and deceleration activities [1,2,5].
Assessment of the daily training load (TL) in professional soccer is well reported, however, within the soccer specific literature TL metrics are reported in isolation and without link to competition [3]. Accordingly, Jaspers et al. [6] state that despite the availability of more detailed TL indicators, the utility of these variables in relation to training outcomes is rare. Currently within soccer team training is usually completed collectively without a position specific orientation, this is despite each individual responding differently to similar TL [7,8]. Furthermore, reporting collective data to improve knowledge and enhance efficiency is vital, however, understanding the positional and individual differences within the sport in terms of specific movement demands imposed upon elite professional soccer players in training and match play is of paramount importance [1]. Although important with its role in developing preparedness for competitive match play, research surrounding the positional training outputs linked to match demands is currently very limited.
The link between training and competition from a periodisation perspective has gained more interest over recent years [9] however, further reporting TL with a direct link to performance preparation is underreported. Tapering training approaches to elite level soccer may facilitate longitudinal progressive adaptations and concurrently reduce injury risk [10,11]. Reporting this information as a multimodal approach in line with positional roles in direct relationship with competition demands could potentially provide a more effective monitoring approach when developing or planning training content. Like many team sports, soccer has a degree of positional variation with regard to training and matchplay demands. Indeed, central midfield (CM) players and full backs (FB) have been shown to cover significantly greater distances than central defensive players in both training and competitive match studies [9,12-14]. Literature suggested that this is may be attributed to these positions tactical roles during match play, resulting in reductions in wellness [2] and increase muscular damage [2] and decrements in neuromuscular qualities [2] meaning that managing the training elements of all positions and ensuring appropriate recovery in order to ensure positive competitive performance is of paramount importance to coaches and practitioners. Barnes et al. [13] observed that across a seven-season period, high-intensity running distance and high-intensity actions increased by ~30% (890±299 vs. 1,151±337m, p<0.001; ES: 0.82) and ~50% (118±36 vs. 176±46, p<0.001; ES: 1.41), respectively. Furthermore, Central midfielders have consistently been found to cover the greatest total distance whilst full backs, central midfielders and wide midfielders run greater distances at high intensities [14,15,16]. Various reasons have been proposed as to why these positional differences in locomotive patterns exist. Research demonstrates that positional differences in maximal oxygen uptake (VO2max) are evident for soccer players, with central midfielders and full backs displaying the highest values [17] whist others found no differences [18]. Nevertheless, central midfielders and full backs consistently have the greatest physical capacities when assessed using intermittent running tests [17,19]. In a report of TL positional differences across a microcycle [9] reported positional differences across TL variables with respect to days preceding competitive match play. Furthermore, it was revealed that CD recorded lower total distances covered (TDC) when compared to CM and WF concurring with previous literature in this area. Interestingly, CF and WF player’s work-rate is generally characterized by intermittent bursts of HI activity, usually into space to receive a pass form a supporting player or sprinting onto a penetrating pass between the opponent’s defence [9]. However, this may also be based on their tactical roles to press the opposition and win back possession leading to greater TDC and HI distance. Given that much of soccer specific training performed collectively as a team, although substantial positional differences exist, it is crucial that an individualised, positional specific approach is adopted, reported and understood within a training context. Being able to provide clarification of the TL across a microcycle may lead to a more comprehensive understanding of positional tapering approaches in elite soccer to ensure positive performances during match-play. Therefore, the primary aim of the current investigation was to examine the position specific intricacies of a novel, contemporary and clear positional multi-metric method examining a tapering structure in-season.
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Methods
Participants
Twenty-nine (n=29) senior male European professional soccer players (26.7±4.07yrs, 183.4±5.87cm, 78.4±8.03kg, 57.55±5.32mL·kg-1·min−1 and 54.12±13.65mm) were assessed throughout the investigation. Five of the players involved within this investigation were members of their respective national team. Players were grouped into positional units for assessment throughout the investigation: Full Backs (FB) (n=6), Central Defenders (CD) (n=6), Central Midfielders (CM) (n=5), Wide Forwards (WF) (n=8) and Central Forwards (CF) (n=4). Informed consent and medical declaration were obtained from participants in line with the procedures set by the local institutions research ethics committee. The study was fully approved by the involved Sports Science and Medical Department at the Football Club.
Procedures, content and study design
The study was conducted over a 22-week mesocycle period during the mid-phase of the competitive season. This mid-season phase was used for the assessment as to ensure minimal fitness changes and subsequent metric changes per training sessions. Daily analysis from training weeks situated within a 1-game week were used within the study to track the positional demands across a regular tapered micro-cycle. 2-game weeks were not assessed in this study due to the TL being significantly different when compared with 1-game training weeks [20]. Players included within the data collection were selected based on their positions within the team but were inclusive of both starting and non-starting players. During the investigation period, all players were instructed to maintain normal daily food and water intake. No additional dietary interventions were undertaken throughout the investigation
Training assessment
Throughout the investigation, data from 88 training sessions used for analysis. The data assessed included 22x1-week blocks of full training sessions per player, per position: 22xmatch day-4 (MD-4) which describes the training session 4 days preceding the match, 22xmatch day -3 (MD-3), 22 x match day-2 (MD-2), and 22xmatch day-1 (MD-1). No other data pre-MD-4 was considered for analysis as they did not have any specific on pitch content of adequate TLs. All training sessions within the investigation peri od were played on an outdoor grass training field with an average temperature of 12.7±2.4 °C. Goalkeeper’s data were excluded from the study and the data analysis. All players were fully familiarized with the type of training sessions and use of GPS. Training sessions were preceded by a standardized warm-up period of 15min and player data were assessed and compared with respect to their individual playing position post training sessions. The microcycles selected for assessment due to these microcyles including full-training weeks following a 1-game period in order to minimize the potential for accumulative fatigue build-up causing variations across specific metrics.
Player running analysis (GPS data)
Each individual player’s running profile each match and training session were analysed using a 10-Hz GPS device (S4, Catapult Innovations, Melbourne, Australia). The current system has previously been shown to be a valid and reliable for the assessment of intermittent movement profiles [21]. For the purpose of the study, the 4 key variables recorded throughout the sessions included TDC, HSR (19.8-25.2 km.h−1), sprint distance (SPD: >25.2km.h−1) and the sum of high intensity efforts which is described as the total number of high accelerations and decelerations (Sum A: D >4m.s-2). All variables recorded for assessment were reported in both absolute and relative terms. For the purpose of this study, the specific speed thresholds set were in line with previous research [9] and the average values per player taken from the com complete training sessions were used for analysis in relation with their maximum competitive match play data. Maximum match day data was used as the benchmark for the microcycle analysis in order to represent the data as a percentage of the player maximal capacity and physical potential. The average values per player, per position were taken from the complete training sessions and used for analysis.
Load management analysis (GPS data)
With the aim of attaining a global daily and weekly volume+intensity score for each playing position, a contemporary load management analysis technique as used by Owen et al. [9] incorporating 4 volume based (TDC; HSR; Sprint Dis; Sum of HIE) and 4 intensity (TDC. min; HSR. min; Sprint Dis. min; Sum of HIE. min) GPS metrics. In order to attain the daily volume and intensity scores, average individual player data was pooled (i.e. as shown in the below calculation) to provide a squad average to create an initial session metric outcome score (Table 1). The mean individual data assessed was then compared to the average maximum individual competitive match play metric achieved and reported as a percentage figure in positional units. The individual percentage volume and intensity of the session were generated through the following calculation:
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statistical analysis
All variables are reported as mean±SD unless stated. Prior to analysis all variables were assessed for normality with the Shapiro- Wilk test. A 1-way analysis of variance (ANOVA) was used to analyse difference in the GPS metrics between playing positions. Another 1-way ANOVA was used to analyse differences between GPS metrics, for each playing positions. When p was found significant (p<0.05), paired t-test comparisons were used to determine differences between the five playing positions and between the eight metrics. The interaction positions: day was also analysed using a two-way ANOVA and t-test paired comparisons were used to compare the playing positions within the days. Holm’s Bonferroni method for controlling type 1 error [22] was also used, therefore a p<0.005 was considered significant for the ten between-positions comparisons, and a p<0.0017 for the twenty-eight between-metrics comparisons. The effect sizes (ES) were calculated for all paired comparisons and evaluated according to [23] as small: <0.50, moderate: <0.50-0.80, and large: >0.80.
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Results
Daily average volume session score was 29±26% and daily average intensity session score was 28±26%, for all players. Over these averages, CF had lower intensity score than CB (ES: 0.28), FB (ES: 0.20) and WF (ES: 0.24) (p<0.005) (Figure 1). Differences were also found amongst playing positions across the different metrics (Figure 2, Table 2). In %TDC and %Sum A:D, CM had lower score than all others (ES: 0.24-0.48); in %HSR, CB had lower scores than CM (ES: 0.39), FB (ES: 0.34) and WF (ES: 0.36); in %SpD, CF had lower score than FB (ES: 0.26); in %TDC. min, CF had lower score than CB (ES: 0.54), FB (ES: 0.52) and WF (ES: 0.42); in %HSR. min, CM had higher score than FB (ES: 0.25), and WF higher than CB (ES: 0.32), CF (ES: 0.44) and FB (ES: 0.44); in %SpD. min, CF had higher score than FB (ES: 0.33), and CB higher score than CM (ES: 0.41), FB (ES: 0.48) and WF (ES: 0.44); and in %Sum A: D. min, CB, FB and WF had higher score than CF (ES: 0.36-0.61) and CM (ES: 0.25-0.48), and WF higher score than FB (ES: 0.24) (p<0.005).
*Significantly different from all other playing positions (p<0.005) $Significantly different from CB (p<0.005) £ Significantly different from CM (p<0.005) # Significantly different from FB (p<0.005) &Significantly different from WF (p<0.005)
Over the day-to-day perspective, FB had higher volume session score than CB (ES: 0.42), CF (ES: 0.45) and CM (ES: 0.38) in MD, CB had higher intensity session score than CF (ES: 0.54) and CM (ES: 0.35) in MD-3, and WF had higher score than CF (ES: 0.34) in MD-3 (p<0.005) (Figure 3). In the interaction positions: days, no significant results were observed in %SpD, %Sum A:D, %TDC. min, and %Sum A:D.min metrics. Differences in %TDC, %HSR, %HSR. min and %SpD. min is presented in (Figures 3-7) respectively. The differences across GPS metrics according to the playing positions are presented in Table 2. Across averages, and in all playing positions, %TDC. min > %TDC with no difference for CF. Across all positions similar scores for %Sum A: D. min were recorded except for CF (ES: 0.33-0.44) who were observed to have increased %Sum A:D. Lower %SpD. min were (p<0.0017) observed for all positions except CB (ES: 0.23-0.34). Additionally, the %HSR was similar across all positions except for the higher observed values for CM (ES: 0.22) and WF (ES: 0.29). Finally, similar %SpD were observed for al positions except CM (ES: 0.32) and WF (ES: 0.34).
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Discussion
The primary aim of the current investigation was to examine a novel, contemporary and clear multi-metric method to better examine the positional tapering strategies of an elite European soccer during an in-season period. The main finding of the current investigation was the identification of a link between playing position and match running performance in elite soccer players which is in-line with previous research [24,25]. It is pretty well-established that wide midfielders cover more total and high-intensity running than central defenders [26-29] and that HI running, and average HI distances increase during the most intense period of matches, with this increase dependent on positional roles and tactical responsibilities within the game [30]. Our findings suggest that significant positional differences exist for TL variables with respect to days preceding competitive match play (i.e different positional tapering strategy).
The current data show large differences in CM players and all other positions within training environments when comparing TDC as a percentage of MD data. These data highlight that midfielders TDC training response is significantly lower when compared to other positions of play. These findings maybe as a result of the increased TDC within game play for midfielders when compared to other positions. Indeed, previously CM have consistently been found to cover the greatest total distance during game play [14-16,28]. Furthermore, these positions have been shown to have increased CK and reduced CMJ during the training week due to increased workload during game play [9]. Overall these results have practical significance for coaches suggesting that when using TDC and as a percentage of MD data as a TL variable of interest coaches will be able to identify if specific positions are appropriately loaded prior to match play. For example, within the current investigation there appears to be a specific underloading of CM when compared with other positions of play.
Consistent with the above findings for TDC as a percentage of match plat we show that large difference exists between the loading prescribed for CM when contrast against all other positions within training when analysis %sum A:D as a percentage of MD data. The current findings highlight how the assessed metric (%sum A:D) training response is significantly lower for midfielders when contrast all other positions. The above findings can be related to the increased TDC in game play for MD players when compared to all other positions [29]. Indeed, these MD players have tactical duties both in attack and defence acting as link players in attack while also providing additional cover for defensive players in defence. These tactical roles may result in greater changes of directions influencing the A:D observed [13].
From the data resented we observed that when assessing the total positional average across the microcycle as a percentage of maximum match day data, CF covered significantly less average intensity scores when compared with CB, FB, and WF players. These findings suggest that following this type of microcycle training structure CF, may require an additional training stimulus such as top up conditioning through small-sided games of high intensity shuttle running, to increase both the intensity and volume markers of these positions in terms of maximum match day output [31,32]. However, this should be completed in accordance with assessments to ensure performance preparation is not hampered and injury risk is increased [9]. When the volume-based metrics were considered we observed that CB and CF positions were significantly underloaded with regard to HSR in training when assessed as a percentage of MD data. These findings are of great importance to coaches as these positions are directly associated with more explosive actions that directly affect the game through creating or preventing scoring opportunities [5,9,16]. As a result of these findings, ensuring these positional demands are induced with sufficient HSR exposures within the training content is vitally important not only for performance preparation, but from an injury risk perspective. Previously Malone et al. [9] revealed that team sports players exposed to sufficient maximal velocity bouts reported significantly reduced injury risks compared to those players who were under or overloaded with the same stimulus. When positional volume comparisons of SpD across the microcycles were analysed, we observed that CF performed significantly less volumes when compared with FB but not any other positions. Again, these findings may suggest that CF who are generally involved in high explosive actions, may require extra SpD volume stimulus, or adversely, the FB require a reduced amount of SpD volume across the microcycle. Understanding the performance profiles, injury rates and tactical efficiency of the players will however, determine whether or not this is correct in terms of load management, with soccer players have been shown tolerate increased SpD during training with increased intermittent aerobic capacities [9]. Our data reveals that when TDC ·min is considered by coaches as a TL volume measure that CB, FB and WF perform increased volume when compared with CM players with respect to percentage of match day values. This can be related to the increased intensity of training with respect to these positions when compared to competitive games. The fact the CM positional role induces a greater TDC based on the role employed, can be related to the consistent higher running performance requirements of these positions. Recent literature has highlighted CM covering greater TDC in games and training and higher intensity of play reported in m·min-1. However, to date no current literature have reported the data as a percentage of match day data. The main practical application of these observations is that CM perform significantly less intensity in training when directly related to match data and compared across positions. This could potential mean that these positions require additional conditioning top-ups during specific periods of the season [9].
One of the most interesting findings from reported data using this contemporary positional method of analysis is the fact that CBs, although largely described in the literature as performing less HSR, sprint distance and TDC within games when compared across positional roles [29] within the current investigation CBs performed significantly greater SpD·min-1 values as a percentage of match performance within training environments. This is interesting as it again highlights that the microcycle training pattern used in this study induced a higher sprint demand for CBs in proportion with other positions assessed. The current observation was consistent with MD-3 findings where CBs were shown to have a higher total intensity scores than CFs and CMs respectively. These findings may highlight an overload with regard to total intensity for CBs on MD-3 within the current investigation. These observations show the practical nature of the current contemporary approach to monitoring training load by revealing the specific positional training load demands of players relative to match play intensities. The findings therefore could potentially allow coaches to better adapt training load in the following days for both the position and the individual. Furthermore, on MD-1, CF covered slightly but significantly higher %TDC than CBs, CMs and FBs (p<0.005) and CMs lower %TDC than all others (p<0.005). CFs and CMs were also largely described in the literature as the playing positions covering the least and the most distance, respectively. Therefore, the high and low proportion of TDC observed for these playing positions, respectively, was not an alarming indication and should not be interpreted as a training overload, even though these values were recorded only 24hr prior to competition. The current investigation should be considered with a number of limitations, firstly, it was not possible to collect information relating to the lower limb non-contact injury occurrence across the period. Future research should aim to assess the impact of the application of the current contemporary model with regard to the injury-workload relationship. Given that objective measures of internal load have been related to fitness increments in team sports athletes, future research should aim to apply the current model to internal measures of load and assess their association with changes in fitness changes across a training period.
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Practical Application
The current study proposed a position specific contemporary multimodal method to represent a player specific tapering and periodisation strategy for both volume and intensity markers across an in-season period amongst professional European soccer players. We have shown that significant differences exist between TLs (i.e., volume and intensity) across both positions and microcycles assessed when representing TL as a percentage of match play, resulting in both overloading and underloading in relation to MD values for specific positions. Using this specific multi-modal approach may allow practitioners to combine key mechanical volume and intensity metrics assessed as part of their athlete or player monitoring strategies to vary the physical stressors depending upon the specific tapering or periodisation approach followed for a given microcyle period. To conclude, this monitoring strategy brings together the key reported metrics from a mechanical perspective revealing a positive portrayal of a periodisation or tapering strategy in order to identify differences between intensity and volume markers across the positional lines of play within an elite European soccer team. The present study demonstrates that the use and integration of a multimodal approach to monitoring TL in direct relationship with competitive soccer match-play at the elite level provides a better weekly review of both player intensity and volume markers for specific positions and can be utilised by coaches to understand the overloading or underloading of specific positions during a microcycle. The current model could be further enhanced by understanding the day-to-day player variance, fitness and wellness state in accordance to the coaching demand corresponding to match-day activity. Continual TL monitoring across the pre- and in-season phases should be performed to prevent players from overreaching and overtraining. Coaches and physical conditioning staff may also further use this method in order to understand which important key metrics need to be stressed or developed to better prepare individual players for match play demands, additionally the model may provide coaches with a better approach to topping up substitute based players who do not complete match day volume or intensity with regard to running performance measures.
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Acknowledgement
This manuscript is original and not previously published, nor is it being considered elsewhere until a decision is made as to its acceptability by the Editorial Review Board. There are no funding sources and are no conflicts of interest surrounding this scientific investigation.
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The Roles of Extracellular Purinergic Signaling in Local Acupoints In Chrono- Acupuncture Analgesia
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Objective: To explore the peripheral mechanism of chrono- acupuncture with respect to purinergic signaling in local acupoints. Methods: The Sprague Dawley (SD) rats were randomly divided into two groups. One group was injected 0.1ml saline as control group. Another group was established by subcutaneous injecting 0.1ml complete Freund’s adjuvant (CFA). After modeling successfully, model rats were randomly divided into model group and acupuncture group based on the basic pain threshold. The basic pain threshold was tested by tail-flick method. The extracellular ATP concentration in local acupoints was detected by high- performance liquid chromatography (HPLC) after acupunctured at different times, which were zeitgeber time 0(ZT0) (7:00), ZT4(11:00), ZT8(15:00), ZT12(19:00), ZT16(23:00), and ZT20(3:00) respectively. Based on the characteristics of the variation of pain threshold and extracellular ATP concentration, the peak phase and valley phase were selected for the further experiment. The protein expression of P2X3 receptors in skin of “Zusanli” (ST36), dorsal root ganglion (DRG) and spinal dorsal horn (SDH) were separately detected by immunohistochemistry. Results: Time had an evident influence on pain threshold (P=0.047, P<0.05) which indicated the pain threshold at different time points was different. The variation of pain threshold among control group, model group and acupuncture group were significantly different (P<0.01). In acupuncture group, the of peak value and valley value appeared at ZT8 and ZT16 respectively. Pain threshold showed complex interactions with extracellular ATP, time factor and acupuncture stimuli. The extracellular ATP concentrations were significantly different in comparison among control group, model group and acupuncture group respectively. (P<0.01). As for protein expression of P2X3 receptors in skin of “Zusanli” (ST36), compared with control group, it obviously increased in model group at ZT8 and ZT16. And there was significant difference between control group and model group (P<0.01). In addition, compared with model group, it obviously decreased after acupunctured at ZT8. And there was significant difference between model group and acupuncture group at ZT8(P<0.01), but not at ZT16 (P>0.05). Interestingly, the protein expression of P2X3 receptors in both DRG and SDH was consistent with that in skin of “Zusanli” (ST36). Conclusion: Temporal variation of purinergic signaling participated in the initial mechanism of chrono-acupuncture analgesia in local acpoints. Also, that might be the base of dynamic variation of acupoints reactivity.
Keywords: Circadian rhythm; Chrono-acupuncture analgesia; Pain; ATP; P2X3 receptor
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Introduction
Circadian rhythm was one of life characters. Peripheral clocks possessed by almost all cells [1]. Circadian clock could be entrainmented by external environments, such as darkness and light, the seasons, the social behaviors, acupuncture and so on [2]. circadian clock encodes key regulators of response to therapeutic treatment [3]. Various of pain syndromes not only showed circadian rhythm, but also affected the circadian rhythm of the body. Most migraine attacks begin in the early morning [4]. many patients with RA present a circadian rhythm in symptoms severity with a significant worsening in the morning. Additionally, studies have shown that the change of pain threshold had obvious circadian rhythm [5-6]. It has been recognized that acupuncture, as an effective means of treatment, could relieve various kinds of pain syndromes [7- 8]. The electro-acupuncture has regulation effects on circadian rhythm of temperature and melatonin in depression rat model [9]. Therefore, the curative effect of acupuncture at different times was different. In terms of chrono-acupuncture analgesia, the studies, pay more attention to central mechanism. Compared with the normal acupuncture, premature acupuncture for the regulation of dysmenorrhea model rats’ β-EP content and HSP70 expression in hypothalamus and pituitary more obvious effect. That might be related to mechanism of central analgesia [10]. However, the peripheral mechanism of chrono-acupuncture analgesia, so far, poorly understood.
Acupoints, as the base of acupuncture stimulation, were the initial taches of acupuncture therapy. The temporal specificity of acupoint reactivity is one of important influence factors of acupoint reactivity. Some studies had shown that purine and its receptors involved in acupuncture analgesia. Acupuncture could increase the concentration of extracellular purine in local acupoints of rats, and the concentration of extracellular purine reached peak value at 30 min, then decreased gradually [11]. P2X3 receptor, as a member of non-selective ATP gated ion channel, participated in the conduction of peripheral painful information through binding to extracellular ATP [12]. Also, the protein and gene expression of P2X3 receptors in DRG increased in various kinds of painful animal models, accompanying with the change of ATP current [13-15]. While the gene expression of P2X3 receptors and ATP current in DRG had showed a downward trend after electro-acupuncture [16]. What’s more, the extracellular purinergic signal, such as ATP, not only had its own circadian rhythm, but also showed the function of time modulation. Thus we proposed the hypothesis that, in the peripheral mechanism, the acpoint reactivity of chrono-acupuncture analgesia might be related to the temporal changes of purinergic signaling in local acupoints.
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Results
The relationship among time factor, ATP & pain threshold on chrono-acupuncture
There were no significant differences among the weight and basic pain threshold of each rats (P<0.05). In control group, the pain threshold peaked at ZT0 and reached the bottom at ZT12 which entails time had a notable effect on pain threshold (P<0.05=. After modeling, both the circadian rhythm of pain and the phase of the pain threshold had changed. compared with control group, the pain threshold of model group decreased. It showed that it peaked at ZT8 and reached bottom at ZT16. However, time had no obvious effect upon the pain threshold in model group (P=0.076,P>0.05). After acupuncturing, compared with model group, the pain threshold of acupuncture group increased. The peak occurred at ZT8 and the valley occurred at ZT16. Time factor had a significant influence on pain threshold of acupuncture group(P<0.05) Table1- Table 5, Figure 1. The interaction played a notable part in pain threshold. When compared the pain threshold among control group, model group and acupuncture group, they were significantly different (P<0.01) Figure 2. The temporal variation tendency of pain threshold in each group. In the control group, two valley value were at ZT4 and ZT12 respectively. The temporal variation of pain threshold in model group showed abnormal pathological changes. While, compared with model group, the pain threshold of acupuncture group showed upward and it also showed abnormal pathological changes.
Time factor had effects on extracellular ATP concentration in different groups. There was significant difference in the variation of extracellular ATP concentration among control group, model group and acupuncture group (P<0.05). There was statistical significance between control group and model group at ZT12 (P=0.017, P<0.05). Extracellular ATP concentration in acupuncture group was higher than that in model group at all six time points. However, there was only statistical significance at ZT16(P=0.022, P<0.05). In the same group at different time points, extracellular ATP concentration also had the differences. In the control group, the extracellular ATP concentration of ZT8(P=0.016, P<0.05) and ZT12 (P=0.038, P<0.05) were significantly different from that of ZT4 respectively. In the model group, the extracellular ATP concentration of ZT4(P=0.026, P<0.05) and ZT20 (P=0.030, P<0.05) were significantly different from that of ZT0 respectively. In the model group, the extracellular ATP concentration of ZT4(P=0.010, P<0.05) and ZT20 (P=0.029, P<0.05) were significantly different from that of ZT12 respectively. And the increase of extracellular ATP concentration in acupuncture group was affected by the time factor, which was statistically significant compared with other two groups Table 6- Table 9 Figure 3 and Figure 4. The trend of extracellular ATP concentration over time. In control group, two valley value appeared at ZT4 and ZT12 and two peak value appeared at ZT0 and ZT8. Extracellular ATP concentration was in a low level at ZT12 and began to show an upward trend at ZT16. In model group, that showed one valley value and one peak value, which was ZT4 and ZT8 respectively. Compared with control group, extracellular ATP concentration abnormally increased at ZT8 and the valley value disappeared at ZT12. The trend of acupuncture group was similar to that of control group. Two valley value appeared at ZT4 and ZT12 and two peak value appeared at ZT0 and ZT8. The time of high point and the low point of extracellular ATP concentration appeared to be normal. The extracellular ATP concentration in acupuncture group was higher than that in model group.
* mean that using ZT12 of control group as a reference △ mean that using ZT16 of model group as a reference # mean that using ZT4 of control group as a reference ▲ mean that using ZT0 of model group as a reference ■ mean that using ZT12 of model group as a reference
a. Predictors: (constant), group, ATP b. Dependent variable: pain threshold
a. Predictors: (Constant), Group, ATP b. Dependent variable: pain threshold
a. Dependent Variable: pain threshold The pain threshold was mainly affected by extracellular ATP concentration in local acupoints and showed a significant positive correlation (P<0.05) Table 10- Table 12.
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The Relationships Between Time Factor and P2X3 Receptors on Chrono-Acupuncture
As for protein expression of P2X3 receptors in skin of “Zusanli” (ST36), compared with control group, it obviously increased in model group at ZT8 and ZT16. And there was significant difference between control group and model group (P<0.01). In addition, compared with model group, it obviously decreased after acupunctured at ZT8 and there was significant difference between model group and acupuncture group at ZT8(P<0.01), but not at ZT16 (P<0.05). Interestingly, the protein expression of P2X3 receptors in both DRG and SDH was consistent with that in skin of “Zusanli” (ST36) Table 13- Table15, Figure 5- Figure 10.
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Discussion
This work indicated that time factor was an important regulator of purinergic signaling in acupuncture analgesia. We found that: 1. Acupuncture not only increased pain threshold but had the function of temporal regulation on pain threshold. 2. The variation of extracellular ATP concentration in local acupoints was affected by acupuncture in different time points and there was a positive correlation between pain threshold and extracellular ATP concentration. 3. The variation of protein expression of P2X3 receptors in local points, DRG and SDH had temporal difference after acupunctured at different time points
Together, our results showed that, in the peripheral mechanism, the acupoint reactivity of chrono- acupuncture analgesia might be related to temporal changes of purinergic signaling in local acupoints. Both master clock and peripheral clock could modulate the circadian rhythm of lives according environment time cues. It was found that the mechanism of molecular oscillations in the peripheral biological clock was similar to that of the master clock [17]. If there were abnormalities in circadian rhythm of the body, chrono-biological indicators changed accordingly. There were some special rhythm characters in many kinds of diseases, especially in pain syndromes. As for rodents, the responses of pain were drastic in dark and moderate in light [18-19]. In clinical studies, pain intensity and body response had rhythmic and cyclical changes. Multiple signaling involved in the rhythmic changes in pain syndromes [20]. Our findings indicated that acupuncture not only increased the inflammatory pain threshold in rats, but also the acupuncture-time interaction had a significant effect on the pain threshold. At different time points, the peak value of pain threshold in control group appeared at ZT0 and the valley value appeared at ZT12. However, the rhythms of animal pain changed after model establishment. The pain threshold of model group was significantly lower than that of control group at ZT0, ZT16 and ZT20 respectively, except at ZT12. Additionally, the peak value of pain threshold in acupuncture group appeared at ZT8 and the valley value appeared at ZT16. Compared with model group, the pain threshold of acupuncture group at the same time point was upward generally. On the whole, time factor was markedly effect on three groups in this experiment. The interaction had significant influences on the pain threshold. Based on experimental results, the pain threshold of rats was rhythmic in physiological conditions. While the pain threshold had abnormal rhythm changes after establishment. And acupuncture could increase the inflammatory pain threshold in rats. The interaction of acupuncture and time factor had significant effect on the pain threshold. In other words, the changes of pain threshold aroused by acupuncture at different times was significantly different. Studies focusing on the extracellular purinergic signaling, represented by ATP, was important informational substances in the body’s temporal structure. In both peripheral and central oscillatory system, extracellular ATP and its metabolites showed marked circadian rhythm. Under the cycle of LD12:12 and DD, the extracellular ATP level in SCN neurons of rats had obvious rhythmical changes. While the extracellular ATP accumulation in vitro cultured cortical glial cells also showed diurnal oscillations [21-22]. The circadian variation of ATP hydrolase activity was of importance for maintaining the temporal function of extracellular purinergic signaling. ATP hydrolase activity in peripheral blood also had rhythmical change, and the highest reactivity occur in dark period [23]. Furthermore, extracellular ATP could selectively raise the expression of gene mPer1 to affect the basic working pattern which was transcription- translation negative feedback among molecular clocks by activating P2X7 receptor [24]. So, extracellular ATP not only outputted temporal information, but also had the function of temporal modulation. Our findings indicated that, the extracellular ATP concentration in control group had double peak (ZT0 and ZT8) and double valley (ZT4 and ZT12). The extracellular ATP concentration of ZT12 was at a low level and began to increase at ZT16. However, the model group showed only one peak (ZT8) and one valley (ZT4). Compared with control group, there was an abnormal increase at ZT8 and the original valley at ZT12 disappeared. The variation of acupuncture group is similar to that of control group and showed double peak (ZT0 and ZT8) and double valley (ZT4 and ZT12). The peak and valley value of extracellular ATP concentration tended to be normal. And the extracellular ATP concentration in local appoints in acupuncture group was higher than that of model group. Combined with pain threshold and extracellular ATP concentration, our results showed that the pain threshold was mainly affected by extracellular ATP concentration in local acpoints and shows positive correlation. There was also a positive correlation between processing methods (whether the rats were in healthy conditions or not and whether acupuncture was adopted).
The studies showed that ATP involved in the pain conduction. P2X3 receptors, a non-selective ligand gated cation channel, played a key role in peripheral transmission of pain [12]. ATP could induce mechanical hyperalgesia in gastrocnemius of rats to release proinflammatory cytokine by activation of peripheral P2X3 receptor [25]. ATP was released from injured cells and excited peripheral P2X3 and P2X3 receptors to generate action potentials of primary afferent neurons, transmit them to the distal end of the central nervous system and released the neurotransmitter’s effect on the postsynaptic process of the central nervous system Neurons [26]. Related studies had shown that P2X3 receptor-induced pain could be significantly reduced after blocking the P2X3 receptors’ conduction pathway or using P2X3 receptor antagonists. After sciatic nerve was cut off, the expression of P2X3 receptor in L4/5 DRG decreased by 50% [27]. It had found that, TNP-ATP, a P2X1, P2X3 and P2X2/3 receptor antagonist, significantly attenuates inflammatory hyperalgesia in the rat model of inflammatory pain induced by carrageenan in the temporomandibular joint (TMJ). Whereas P2X1 receptor did not participate in this model pain, which mean that P2X3 and P2X2/3 receptor might involve in the inflammatory pain sensitivity of the rat model of TMJ [28]. A-317491, a selective P2X3 receptor antagonist, could reduce chronic inflammatory and neuropathic pain mediated by P2X3 receptor in rats. And intrathecal administration of A-317491 seemed to be more effective to relieve tactile allodynia after peripheral nerve injury than intraplantar administration [29]. Meanwhile, many
studies showed that, purinergic signaling played an important role in acupuncture analgesia. Electro-acupuncture could exert an analgesic effect on neuropathic pain through simultaneous action of purine Al and P2X3 receptors [30]. Also, acupuncture could reduce the expression of P2X3 receptor gene and the magnitude of ATP excitation current in DRG of rats with chronic constriction injury (CCI) [31]. What’s more, midbrain periaqueductal gray P2X3 receptors involved in the modulation mechanism of electro-acupuncture analgesia in the spinal cord. ATP had circadian rhythm and had the function of temporal modulation [32]. Our findings indicated that, no matter in skin of “Zusanli” (ST36), DRG and SDH, compared with control group, the protein expression of P2X3 receptors in model group markedly increases at ZT8 and ZT16 respectively. While compared with model group, the protein expression of P2X3 receptors in acupuncture group markedly decreases at ZT8, but not at ZT16. These results mean that, there was temporal difference in decreasing the protein expression of P2X3 receptors by acupuncture. Moreover, after both establishing and acupuncturing, the variation of protein expression of P2X3 receptors in DRG and SDH displayed highly consistency at the same time point. This might be one of the foundations of the time effect of acupuncture analgesia
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Experimental Procedures
Animals
The experiment consisted of two parts. 156 SD male rats were needed in this experiment. All rats (body weight of 200 ±20 g) were provided by animal center of Sichuan provincial hospital (Permit Number: SCXK(CHUAN)2013-15). The rats were acclimated to the rat facility for 1 week before starting the experiments. The rats were housed under 12h:12h light/ dark cycles (Light on at 7:00, ZT0 and off at 19:00, ZT12) with free access to water during the whole experiment, and free access to food. Animal care was carried out in accordance with the Instruction for Ethical Treatment of Animals issued by the Ministry of Science and Technology, China, in 2006. We tried to minimize the number and suffering of the laboratory animals. All procedures and animal experiments were approved by the Animal Care and Use Committee of Chengdu University of Traditional Chinese Medicine (China) (NO.2014-01).
Assessment of basic pain threshold
The basic pain threshold of rats was tested by tail-flick method. The standard rats which tailed between 3 and 10 seconds on the test were involved in the experiment. The basic pain threshold was needed to finish within 1 hour under the same condition. In addition, in order to avoid scalding the rats and calm them down, each one was measured at intervals of 5 minutes or more
Grouping and modeling
In this part experiment,108 SD male rats were randomized divided into two parts. One part was subcutaneously injected 0.1ml 0.9% saline into the right hind paw for 1 day before the experiment as control group. Under the same condition, another part was subcutaneously injected 0.1ml CFA into the right hind paw for 1 day before the experiment. After successful replication of the model, it was randomly divided into model group and acupuncture group based on the basic pain threshold. And each group was divided into 6 sub-groups which were ZT0, ZT4, ZT8, ZT12, ZT16 and ZT20 respectively. The rats established by CFA should be in acute pain stage after 24 hours of injection and the syndromes showed that the time of tail-flick shortened accompanying with red-swollen paw.
Experimental progress
Assessment of pain threshold: At the second day after model establishment, the rats in acupuncture group acupunctured “Zusanli” (ST36) in the affected side for 30 min (twisted 1 min every 5 min, 120 per/min). When the therapy was finished, the pain threshold of rats was tested by tail-flick method. The pain threshold was needed to finish within 1 hour under the same condition. Additionally, in order to avoid scalding the rats and calm them down, each one was measured at intervals of 5 minutes or more
Assessment of extracellular ATP concentration in local acupoint: We collected samples of interstitial fluid by a micro dialysis probe implanted in the tibialis anterior muscle/sub cutis of rats at a distance of 0.4–0.6 mm from “Zusanli” (ST36). The processes of this experiment as follows: 1. Under the constant temperature of 37°C with a CMA/450 animal thermostat, the rats were anesthetized with 10% chloral hydrate (0.4ml/100g) by intraperitoneal injection and kept them in prone position. The right hind leg of the rats was shaved, disinfected and kept operating area clean and dry. 2. The length of the catheter was guided by micro dialysis probe accurately. 3. Cut off the skin where the probe had been marked with tissue scissors and tissue forceps. The wound was minimally invasive as long as the probe was successfully inserted. The syringe needle was needed to insert into the tear supporting tube and was slowly implanted local tissue of acupoints. Then, the syringe needle was put out, and the micro dialysis probe (CMA20, Sweden) was inserted into the tear supporting tube. 4. The two ends of the probe were respectively connected to the microanalysis pump (CMA402) and the cooling microcollector (MAB85). In the micro dialysis pump, 0.9% saline was injected into the injector, with the speed of 1μL/min and balanced for 1 hour. In the cooling micro-collector, the temperature was set to 4°C. After the balance, the local tissue fluid of the acupoints was collected by the cooling microcollector automatically (30min / 1 sample). Each rat was collected for two samples which were respectively before and during acupuncture. The sample collections lasted for 1 hour. 5. The content of purine in the sample collected by the microanalysis was tested by HPLC.
Assessment of protein expression of P2X3 receptors in skin of “Zusanli” (ST36), DRG and SDH: Based on the former experimental results, the peak value (ZT8) and the valley value (ZT16) were chosen for the further experiment. 48 SD male rats were needed in this part experiment. The methods of assessment of basic pain threshold, modeling and grouping were the same as the former. The rats in acupuncture group acupunctured “Zusanli” (ST36) in the affected side for 30 min (twisted 1 min every 5 min, 120 per/min). Meanwhile, the rats in the control group and model group did not accept acupuncture therapy. When the therapy was finished, the rats were anesthetized with 10% chloral hydrate (0.4ml/100g) by intraperitoneal injection and the skin and subcutaneous tissue of “Zusanli” (ST36) in the affected side (size:2*2; depth:2~3mm ), DRG(L4-L6) and SDH (L4-L6) were respectively took away, then immediately placed in 4% paraformaldehyde and examined by immunohistochemistry and average optical density method.
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Statistical Analysis
All data were analyzed by SPSS19.0 and results were presented as mean± standard deviation (SD). Statistical methods which consisted of one-way ANOVA (one-way analysis of variance), RMANOVA (repeated measures analysis of variance) and MANOVA (multivariate analysis of variance) were used to compare between the groups after the normal distribution test and the homogeneity test of varuances. Significance was determined at P<0.05.
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Acknowledgement
This study was funded by National Natural Science Foundation of China (NO. 628313). The authors have declared that no competing interests exist.
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Author Contribution
Conceived and designed the experiments: DC ZZ. Performed the experiments: XL XS SH YW XZ NL. Analyzed the data: QZ JZ. Wrote the manuscript: SH XL XS NL
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Pleiotropic Effect of Herbs: Hepatoprotective and Hepatotoxicity
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Every herb of our mother earth has therapeutic as well as harmful effects, so term Pleiotropic effect of herbs is better stint for defining herbs. Although herbal remedies and their active phytoconstituents have a potential to treat devastating hepatic disorders, but they are also known to possess hepatotoxic properties. Ayurvedic drugs as well as their preparations act in a holistic manner to treat liver disorders via targeting multiple pathways but still there is a need to explore their novel hepatoprotective mechanism along with possible harmful and toxic outcomes. Therefore, it is the responsibility of the user to use herbs as Amata or Sudha not like Visah. Our purpose is not to dampen the use of herbal drugs, but aware all researchers and clinicians for the usage of herbs with good practice
The plants like Andrographis paniculata, Ocimum sanctum, Solanumnigrum, Silybummarianum, Phyllanthus niruri etc. were proved to be have hepatoprotective action [1]. Several hepatoprotective mechanism of herbal drugs have been revealed such as the interaction with various CYP isoforms, capability to decrease oxidative stress via increasing endogenous antioxidants like reduced glutathione, Catalase, level of Phase II/antioxidant enzymes decrease inflammatory mediators’ releases and to inhibit the entry of toxins to the cells [2-4]. Moreover, natural products have shown great promise in combating the toxicity of several commonly used drugs, including acetaminophen and paracetamol. However, like all apathic medicines there are numerous herbals medicines which causes hepatotoxicity due to their large doses and drug abuse. Herbal products including Shou Wu Pian (Polygonummultiflorum), Breyniaofficinalis, Germander (Teucriumchamaedrys), Chaparral (Larreatridentata), Actractylisummifera, Impila (Callilepsislaureola), Pennyroyal (Menthapulegium), Greater celandine (Chelidoniummajus), Kava (Piper methysticum), Black cohosh (Cimifugaracemosa), Noni juice (Morindacitrifolia), Gotu Kola (Centellaasiatica), etc. are reported to have liver damaging effects [5]. These products when consumed cause symptoms ranging from acute, chronic, cholestatic, fulminant, and acute autoimmune-like hepatitis to acute liver failure, and liver cirrhosis. The exact mechanism of their toxicities is largely unknown; however, involvement of oxidative stress and apoptosis is frequently reported [5]. There are numerous validated scientific evidences revealing the hepatoprotective potential of herbs including, polygonummultiflorum thumb. and Radix bupleurion the contrary these drugs are also documented for their hepatotoxicity at higher dosage [6,7]. Therefore, prescription-based bestowing of herbal drugs is certainly required to control herbal induced hepatotoxicity [8]. Additionally, Numerous natural products, are now widely accepted for their hepatoprotective activity and used in various marketed hepatoprotective formulations like Liv 52 (Himalaya Drug Co, Bangalore), LIVOZON M/S Hind Chemicals, Kanpur, Liver Care, 180 Vegetarian Capsules(Himalaya Drug Co, Bangalore), LIVOTRIT (M/S Zandu Pharmaceuticals Bombay) etc. [1]. Today’s demand is to promote the use of natural marketed preparation as supplementary medication but with proper care and practice. However, a majority of natural products investigated to date are non-toxic, but some studies have shown liver toxicity by certain natural products. Therefore, the proper selection of the herbal medicines is also necessary [9]. It is envisioned that natural products will not only lower the risk of drug-induced liver damage, but also provide an alternative solution to remedy the drug-induced hepatotoxicity.
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Competing interest
The authors declare that they have no competing interests.
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Acknowledgement
We acknowledge the Chitkara University for providing facilities.
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The Impact of Emotional Intelligence as Buffer Effect of Stress in the Working with External Clients, The Specificity of the Health Professionals
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Aim: Skills based on EI (Emotional Intelligence) lead to challenges both in the area of personal excellence and professional success, so with this research we intend to explore the relationship between EI and PS (perceived stress) in workers with external clients, particularly health professionals. Materials and Methods: empirical, cross-sectional and non-experimental study, with a non-probabilistic sample by networks of 874 workers from different professional activities (154 healthcare area). The applied questionnaire includes four scales, however in this study we used the EI and PS scales. Results: The scales used with good indexes of adjustment: EI [ /df= 3,965; NFI= .923; RMSEA= .055; α= .84; M= 5.11 (SD= .63)]; PS [ /df= 4.045; NFI = .953; RMSEA = .059; α = .82; M = 1.79 (SD = .51)]. The results support empirically a negative relationship between emotional intelligence and perceived stress in workers, this relationship can be enhanced with the relationship with other people, such as external clients. Conclusion: We conclude that people who contact the general public develop EI more, in turn EI relates has a negative and statistically significant relationship with PS.
Keywords: Emotional intelligence, perceived stress, people management such as external clients
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Introduction
The growing interest in investigating and debating the concept of EI has resulted in a better understanding of the role of emotions in human life, as well as the impossibility of separating emotions from rationality, as this new intelligence construct encompasses several dimensions of knowledge: self-regulation, adaptive impulse control, self-efficacy and social intelligence [1,2]. Studies show that higher emotional intelligence scores lead to reduced burnout and better performance, better communicative ability, more safety and more satisfaction [3]. Therefore, EI is identified as the cornerstone in reducing negative work outcomes and improving employee well-being [4].
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Materials and Methods
This is an Empirical, cross-sectional and non-experimental study, with a non-probabilistic sample by networks of 874 workers from different professional activities in Portugal (154 healthcare area) and 65% of these workers work with external clients. The applied questionnaire includes four scales, however in this study we used the EI and PS scales.
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Results
According to the adjustment indices of the confirmatory factor analysis of this instrument that can be observed in the Table 1, we can see that the models proposed by the authors [5], overall presents good adjustment indices: EI [x2 /df= 3,965; NFI=.923; RMSEA= .055;]; PS [ x2/df= 4.045; NFI = .953; RMSEA = .059].
By analyzing the Table 2 all dimensions of the EI positively correlate with the variable Work with external clients, which leads us to deduce that EI is enhanced with relationships with other people, such as external customers. Due to the specific characteristics of the health area, health professionals present a significant correlation (r= .17 with p < .05) with Empathy and social contagion, meaning that health professionals are the most creative, the most effective at resolving problems, and the best team workers. On the other hand, in the other professional categories, when analyzing Table 2, we find that there is a very strong correlation (p < .01) between the dimension of Self- Encouragement and working with external clients (r= .10). It seems that participants feel that the priority is to belong to enthusiastic teams in order to overcome the obstacles and challenges.
* p < .05 ** p < .01 The PS correlates negatively with the variables it works with external clients both at the global scale level and in the analyzed professional categories. Given the results we decided to test the difference in magnitude of the correlation coefficients between the EI (and constituent factors) and the PS previously obtained in health professionals and other professional categories, the complementary SPSS routine was performed. (Syntax Files), elaborated by Alferes [6], to test the difference between two correlation coefficients of two independent groups (SP, obtained in health professionals and other professional categories). With the observation the Table 3 we can see that there are significantly higher negative correlations in health professionals compared to other professionals, between the SP and the global EI scale, thus, we can conclude that, in general, EI acts as a buffer effect in PS more effectively in health professionals (24%), while only 16% in other professionals. This effect assumes greater expression in the Self-Encouragement dimension (z = 3.67), since the proportion and variance shared with the SP in health professionals is 13%, while in other professionals it is only 4.8%.
*p < .05 ** p .01
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Discussion
The performance of the psychometric studies of the measuring instruments allowed us to demonstrate that the models proposed by the authors, in our sample, have good overall adjustment indices. As for internal consistency, we obtained values considered good. The global scale of EI showed an internal consistency of .84 and the PS scale showed an internal consistency of .82, which are considered good values as described in the literature [7]. To better understand this phenomenon (emotional intelligence as a buffer effect on perceived stress) we added sociodemographic variables (Working with external clients) to our study and studied its effect on emotional intelligence and perceived stress. Controlling the variable Work with external clients, we found that it correlates positively with EI, with more significant relationships in the Self- Encouragement dimension, in the specificity of the professional category, we found that EI in all its constituent factors correlates positively with the Professionals variable. being the most significant relationship with Empathy and emotional contagion. It seems that people in contact with the general public develop EI more, probably because they are more often involved in conflicts, which they have to solve creatively, developing constructive ways to manage anger and maintain difficult conversations [5], in the case of health professionals in which their context is in the high emotional work plan because it involves the perceived and expressed feelings [4]. In this line of thinking, our results were astonishing as healthcare professionals as they work with various types of clients feel the need to train themselves to better serve their clients. However, in our research we found no studies on the influence of working with external clients on EI or SP on health professionals. And indeed, our research is pioneering as we conclude that EI acts as the most effective stress protection factor for healthcare professionals, because considering the overall scale of EI we found a probability of 24% of acting as a buffer effect in SP in health professionals whereas in other professional categories it is only 16%.
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Conclusion
Effectively it was found that IE has a negative impact on perceived stress, i.e., the higher the IE score the lower the SP, this relationship assumes greater magnitude in health professionals. In our view this was an expected result, because in the research done all the literature pointed in this direction. On the one hand, in this relationship, we highlight the dimension Understanding one’s own emotions and Self-Encouragement in health professionals and the Self-Encouragement dimension in other professional categories. On the other hand, the scales used (EI scale and PS scale) showed a good fit index to the data.
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Acknowledgement
We want to convey our deepest thanks to all the people who made this project possible.
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Conflict of Interest
We don’t have any economic interest or any conflict of interest.
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Angiogenesis as A Potential Therapeutic Target for NASH Treatment
Non-alcoholic fatty liver disease (NAFLD) - the most common type of chronic liver disease - encompasses a histological spectrum of disorders including simple fat accumulation (steatosis), non-alcoholic steatohepatitis (NASH) and cirrhosis. NASH is a chronic and silent liver disease characterized histologically by the presence of hepatic inflammation and cell injury (hepatocellular ballooning) due to hepatic steatosis equal or superior to 5% of hepatocytes [1]. NASH develops in the absence of excessive alcohol consumption but is linked to unhealthy eating habits and lack of physical activity [1]. It is often referred to as metabolic disease of the liver. NAFLD patients with obesity and metabolic syndrome features such as insulin resistance, type 2 diabetes mellitus, hypertension and dyslipidemia, are at higher risk of progression to NASH. NASH is associated with higher cardiovascular risk and increased fibrosis leading to cirrhosis and liver cancer when serious [2].
Currently no approved pharmacotherapy exists for the treatment of NASH. Mainly sustained weight loss by a calorie-restricted diet, eating habit and lifestyle modification, and increased exercise are the top priorities and recommendations for patients. Final goal is to achieve and sustain weight loss of 7% to 10% of bodyweight, as this has been shown to improve the majority of histopathological features of NASH [1]. The past decade has been an explosive interest in drug development targeting pathologic pathways in NASH, with numerous phase 2 and 3 trials currently in progress.
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Drugs in Phase 2 And 3 Trials
The development fever of NASH treatment called ‘golden egg’ has not cooled down. Currently, ‘Ocaliva, Obeticholic acid (OCA)’ of Intercept Pharmaceuticals (USA) is the only treatment that has finished phase 3 of clinical trial. However, there are still labels for safety and efficacy issues. This means that the NASH cure, which in turn has a higher efficacy and a more safety, is strongly needed. Currently developing drugs in phase 2 and 3 trials have different mechanism each. The most leading player is OCA, a potent agonist of the farnesoid X nuclear receptor (FXR) that is just being evaluated in the phase 3 study REGENERATE (NCT02548351) for the treatment of NASH after being studied in the phase 2b FLINT trial (NCT01265498), where 283 patients with non-cirrhotic NASH were randomized 1:1 to receive OCA 25mg or placebo for 72 weeks [3].
The next launching candidate is Elafibranor (GFT505). Elafibranor is a dual PPAR-α/δ agonist produced by GENFIT (France) that is currently undergoing evaluation in the phase 3 RESOLVE-IT trial (NCT02704403). Elafibranor was tested in the phase 2b GOLDEN-505 trial (NCT01694849) which randomized 276 patients with NASH without cirrhosis to Elafibranor 80mg, 120mg or placebo groups for 52 weeks [4]. Estimated primary completion date is December 2021. Cenicriviroc (CVC) is a dual CCR2/CCR5 inhibitor owned by Tobira Therapeutics (USA). In animal fibrosis models it is demonstrated to have anti-inflammatory and anti-fibrotic properties. CVC is being evaluated in phase 2 and phase 3 trials. However, big pharmas have experienced continuing failures in developing NASH treatments and terminated clinical trials such as Selonsertib (P3, Gilead, ASK-1, apoptosis signal-regulating kinase 1 inhibitor), Liraglutide (P3, Novo Nordisk, GLP-1, glucagon-like peptide-1 analogue), Metadoxine (P3, General de Mexico, Antioxidant, glutathione source), hydroxytyrosol and vitamin E (P3, Bambino Gesù Hospital and Research Institute, Antioxidant), Emricasan (P2, Novartis, Caspase Inhibitor), NGM282 (P2, NGM Bio, Variant of FGF-19), BMS-986036 (P2, Bristol-Myers Squibb, Pegylated FGF-21), Simtuzumab (P2, Gilead, LOXL2 antibody), Volixibat (P2, Shire Pharmaceuticals, ASBT, apical sodium–bile acid transporter inhibitor) and Saroglitazar (P2, Zydus Discovery, PPAR-α/γ agonist) [5].
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Multi Target
The pathogenesis of NASH is still unclear. Until recent years, a ‘two-hit’ theory has been thought to drive NASH pathogenesis (steatosis of more than 5 % of hepatic fat, as a first hit and other factors such as inflammatory cytokines, mitochondrial dysfunction, and oxidative stress, as a second hit) [6]. However, this view is not generally recognized because NASH can be developed by many other molecular pathways and the driving factors may be different among patients. Thus, ‘multiple-hit’ model is widely accepted now [7]. The presumable multiple factors are as follows: lipid accumulation by excessive delivery from adipose tissue such as visceral fat, insulin resistance, inflammation, gut-liver axis dysfunction, genetic factors associated with inflammation, lipid metabolism and oxidation. These factors affect to liver function to treat energy sources such as carbohydrate and lipid and result in the accumulation of toxic lipid species, leading to stress, damage, death in hepatocyte and following fibrosis, cirrhosis and hepatocellular carcinoma. Based on these hypotheses, it is considered that the development of therapeutic regulating multiple target may be more effective rather than that of specific one target.
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Angiogenesis and Liver Fibrogenesis
Angiogenesis is closely associated with NASH, especially in pathogenic liver as well as in adipose tissue. Fatty acids primarily delivered into liver come from adipose tissue such as visceral fat. Adipose tissue accumulates lipids by the remodeling process including adipocyte differentiation and angiogenesis, and supplies lipid to liver in the form of free fatty acids through lipolysis [8]. Inflammation and excessive free fatty acids due to dysregulation of lipolysis in adipose tissue can induce the NASH pathogenesis [6]. In normal liver, fibrogenesis initiate under acute liver injury but the process is transient and easily recovered. In pathogenic status of chronic liver disease, for example, fibrotic liver, normal oxygen delivery is persistently reduced due to the increase of portal venous resistance by the deposition of extracellular matrix (ECM) [9,10]. Hepatic stellate cells (HSCs), which produce collagen and pro-inflammatory cytokines, are a major culprit of fibrosis. Hypoxia in liver upregulates angiogenic factors, such as vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF) in hepatocyte and HSCs and induce immature angiogenesis. The incomplete vessels cannot overcome hypoxia and furthermore maintain chronic inflammatory condition by infiltrating inflammatory cells secreting pro-inflammatory cytokines tumor necrosis factor alpha (TNFα), interleukin-6 (IL-6), nitric oxide (NO). These conditions again stimulate HSCs and induce the deposition of ECM, leading to liver fibrosis. Therefore, hypoxia, pathologic angiogenesis, and liver fibrogenesis are all interconnected events.
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Conclusion
Although the causes of NASH are not well defined, we now know the presences of many factors and multiple targets in NASH. Angiogenesis inhibitors have the potential to treat NASH targeting liver fibrogenesis. Studies examining antiangiogenic therapy in rodent models of chronic liver disease were undertaken. However, NASH therapeutics that are able to control multiple factors at a time and to treat for a long term are required. Recently, herbal extracts of Melissa [11] and stevia [12] showed the alleviation of NASH pathogenesis through multiple pathways including angiogenesis. Herbal medicine may be a desirable alternative due to its multiple action, long-term safety, cost-effectiveness and good compliance for NASH treatment.
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Effects of Sodium Bicarbonate Supplementation on Repeated-sprint Ability in Professional vs. Amateur Soccer Players
Soccer is described within the research as a high-speed and high-intensity intermittent sport exposing players across many levels to a continued physical, physiological, technical, tactical and psychological demands. This variance of stressors encountered during actual training and competitive match-play have shown fatigue to become a prevalent issue, especially following periods of high intensity bouts. As a result this investigation has been developed in order to compare the effects of sodium bicarbonate ingestion (NaHCO3) on professional and amateur soccer player’s RSA (7 x professional players: mean±SD: age 21.7±2.1yrs; weight 79.7±9.5kg; and 7 x amateur players: mean±SD: age 22.8±1.2yrs; weight 79.3±4.9kg). Each player ingested 0.3g.kg-1 NaHCO3 or placebo microcrystalline cellulose (MC) in a randomized, double-blind, crossover order, 90-minutes before the repeated-sprint ability (RSA) test (5 x 6-seconds maximal-effort sprints). No differences were found in La- concentrations among professional or amateur players in MC or NaHCO3 conditions pre-exercise (P>0.05). The NaHCO3 trial revealed significantly higher post-exercise La-concentrations in professional (9.57±1.09vs. 10.77±0.90mmol/L-1) vs. amateur players (10.06±1.45 vs. 10.87±1.25 mmol/L-1). NaHCO3 resulted in significant improvements in mean power output in sprints 2 (512.3±199.4 vs. 547.6±185.3W) and 3 (468.6±209.4 vs. 491.6±199.0W) in amateurs, but no effect in professionals. Therefore, it may be suggested that amateur participants in soccer may benefit from NaHCO3 ingestion more than professional players as a result of their reduced physical conditioning level when compared to professional level players.
Keywords: Sodium bicarbonate; Repeated sprint; Soccer
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Introduction
Soccer is characterized by its unique, unpredictable, intermittency profile in both training and competitive games [1]. Previous research has suggested that during match play, high intensity efforts last for anything between 3.7-4.4 seconds in duration [2,3]. Furthermore, elite level players complete significantly more sprints than their amateur counterparts (1.4±0.1 vs. 0.9±0.1%, p<0.05) [4]. Recent research has revealed a strong correlation between repeated-sprint ability and elite soccer performance [3]. Energy produced during repeated sprints predominantly derives from anaerobic glycolysis [5], a metabolic pathway limited by the progressive increase in acidity through the accumulation of lactate (La) and hydrogen ions (H+) in muscle and blood [6]. Fatigue during high-intensity exercise remains contentious, with further uncertainty surrounding fatigue in soccer [7]. While the mechanism of H+ accumulation is well documented, other mechanisms are largely involved in the RSA fatigue. Earlier research in this area suggestH+accumulation could result in alterations in enzyme activity, perceived effort, ion regulation; or inhibition of essential glycolytic rate limiting enzymes phosphofructokinase and lactic dehydrogenase [8]. Various intracellular buffering mechanisms become active with increased repeated-sprints in an attempt to neutralize the increased H+ in the blood, including the monocarboxylate transporters 1 and 4, although it is believed bicarbonate (HCO3- ) is the most active [9]. The body’s natural stores of HCO3- are limited; therefore, when the body’s buffering capacity is exceeded through increased H+ production acidosis occurs, resulting in fatigue [6]. Elevated H+ concentrations as a result may increase pyruvate dehydrogenase activity, thus enhancing aerobic participation. Evidence indicates La- and H+ accumulation, and the resultant acidosis are not the sole cause of fatigue [2].
The ingestion of sodium bicarbonate (NaHCO3) prior to exercise is suggested to enhance high-intensity sport performance particularly in sports which involve rapid motorunit activity and large muscle-mass recruitment such as soccer [10]. Induced alkalosis following NaHCO3 ingestion is thought to increase the body’s extracellular buffering capacity, delaying the onset of fatigue, and pH decrease, increasing muscle contractile capacity, through enhanced muscle glycolytic ATP production, and increased muscle La- efflux [11]. Evidence suggests NaHCO3 ingestion~120-minutes pre-exercise in a dosage of 0.3g. kg-1. BM may improve high-intensity sport performance by~2% [12]. However, conflicting reports exist during repeated-sprint protocols with performance enhancement in some [2] but not others [13].
Some suggest physical characteristics have a significant impact upon participant’s responses to NaHCO3 supplementation [14]. Anaerobically trained, elite participants are likely to fatigue as a result of mechanisms other than acidosis during highintensity exercise [15] as they possess higher muscle buffer capacities which blunt the ergogenic effects of exogenous buffers [16]. This may have specific implications on soccer performance, where amateur or youth players, or players returning from injury who display significantly lower anaerobic capacities [17]. A recent meta-analysis [18] concluded that the ergogenic effects of NaHCO3 are diminished among amateur or untrained athletes. Additionally, this is the first study to examine the impact of training status upon the ergogenic effects of NaHCO3 supplementation during repeated-sprint ability exercise. As a result, the purpose of this investigation is to compare the effects of NaHCO3 ingestion on the repeated-sprint performance in amateur and professional soccer players.
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Methods
Experimental approach to the problem
Upon commencement of the investigation, participants completed 3-separate sessions composed of one familiarization session (e.g. laboratory introduction, testing requirements, introduction to testing equipment, practice sprints and anthropometrics) and two testing sessions. Although a familiarization session was performed, the players involved within the study were fully aware of the tests used due to their ongoing club seasonal sport science testing battery [19]. To begin the investigation, players were placed in a randomized placebocontrolled, double-blind crossover design method. Following the ingestion of either 0.3g.kg-1.bm (body mass) of NaHCO3 or 0.3g. kg.bm of microcrystalline cellulose (MC-Placebo) in identical capsules, the players completed 5 x 6-second cycle bursts with 24-seconds passive rest between maximal efforts. Following a 7-day separation crossover trial period, both sets of repeatedsprints were conducted at the same time of day (10:00hrs to 11:30hrs) to control for diurnal effects. Capillary blood was taken from the fingertip before capsule ingestion (baseline), 100-minutes post-ingestion (pre-exercise), and immediately after repeated-sprint (post-exercise). Participants were informed to maintain normal dietary patterns and training throughout the study. Participants were also asked to refrain from consuming food and beverages (other than water) 2-hours before testing, they were also asked to avoid alcohol or any vigorous exercise 24-hours before testing. The experimental design is shown in Figure 1, with the overview of the experimental protocol presented in Figure 2. The experimental protocol has previously been described used in accordance with recent research [2]. This research was approved by the ethics board and research committee within the nominated University and participants were informed of the research requirements, benefits and risks before giving written consent.
Subjects
Fourteen soccer players were tested as part of this investigation (7 x professional players: mean±SD: age 24.7±2.8yrs; weight 79.7±9.5kg; and 7 x amateur players: mean±SD: age 19.9±1.2yrs; weight 79.3±4.9kg). All participants had been involved in soccer for over 5 years at various levels ranging from amateur participation to professional and the testing took place in the mid-season phase as to ensure stable physical player profiles. Participants were excluded from the study if they were taking medication known to affect pH balance, suffering from chronic disease, recently suffered an episode of fatigue/ flu or were currently taking performance enhancing supplements.
Training Status
Participants were assigned to one of two groups based on their training status; characteristics of each group are presented (Table 1). Professional soccer players were assumed to be well trained, whereas amateur soccer players were assumed to be untrained. These assumptions were confirmed by the results of a non-exercise model questionnaire [20], and a high-intensity training questionnaire. Participants were deemed to be well trained if they had a MET of <13 and took part in at least 3 highintensity training sessions each week, for at least 5 consecutive weeks.
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Procedures
Substance ingestion
Participants ingested either 0.3 g.kg-1 NaHCO3 or 0.3g.kg-1 MC in 18-24 gelatin capsules, 120-minutes prior to performing the repeated-sprint test. Capsules were closely matched for weight, sight and size, and were assigned in a randomized, double-blind, crossover manner.
Repeated sprint protocol
Participants performed a pre-test warm up 90-minutes following capsule ingestion consisting of 5-minutes cycling at 80W. This was followed by 3 practice starts, where participants were required to pedal at near maximal speed for 2-3 seconds interspersed with 20-seconds slow pedaling followed by a 90-second rest. Participants then performed a 6-second maximal assessment sprint test on the cycle ergometer (Monark Ergomedic 828e, Sweden). The power output completed in the first sprint was used as the criterion score during the subsequent 5 x 6-second cycle test. The subjects were allowed 5-minute off bike rest following the assessment sprint. The 5 x 6-second cycle test consisted of five, 6-second maximal sprints commencing every 30-seconds, with mean power output recorded for each individual sprint. Participants were required to achieve at least 95% of the criterion score for the first sprint, as a check of pacing. Participants that failed to achieve the 95% criterion score were allowed further 5-minutes rest and recommenced the 5 x 6-second cycle test. The 24-seconds between sprints consisted of active recovery, with participants instructed to maintain a cycling speed of 80 RPM with no load and counted in from 5-seconds before commencing the next sprint. All maximal tests were undertaken using the standard Wingate anaerobic test load (7.5g.kg-1.BW) and procedures [21]. Standardized verbal encouragement was provided to each subject during all sprints, with all sprints performed in the seated position. A similar repeated-sprint has been used previously [2] and has been reported to be a valid and reliable test of repeated-sprint ability [22].
Blood sampling and analysis
Whole-blood La- was taken from finger-prick blood samples and assessed for physiological responses to both NaHCO3 and MC. Participants fingers were prepared for sampling using an alcoholic wipe, dried with a tissue then punctured with a disposable lancet (Owen, Mumford, Oxford, UK). The initial droplet of blood was removed with a tissue; subsequent droplets of volume 5μl were collected on the La strip of the La Pro LT- 1710 (Arkray, Kyoto, Japan). Blood samples were taken on arrival~100-minutes post-capsule ingestion, and immediately post-exercise. The La Pro is considered a viable measuring tool for the analysis of blood La owing to its proven reliability [23].
Gastrointestinal tolerability assessment
Acute GI discomfort questionnaires were completed at rest, pre-exercise and post-exercise. The questionnaire has been used in previous literature [24] and participants were required to report the intensity of sickness and stomachache by selfselecting a number along the scale provided. The scales showed integers from 0 to 10, with descriptors at 0, 3, 6, 9, and 10. The descriptors along the sickness scale included: not at all, slightly, quite, very, and sickness, and along the stomach ache scale: none at all, dull ache on and off, moderate continuous, severe continuous and severe doubled up
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Statistical analyses
All values are reported as mean±standard deviation. Within group difference in blood La, mean power output and GI-tolerability was analyzed using a paired t-test. Independent t-test was used to analyze the percentage change between groups for mean power outputs from placebo to NaHCO3, and absolute difference for La concentrations and GI-tolerability from placebo to NaHCO3. Statistical significance was accepted at P<0.05. Between trial differences were also assessed using Cohen’s effect size with modified descriptors (Hopkins, 2002), using the following criteria:<0.2= trivial, 0.2-0.6 = small, >0.6- 1.2 = moderate, >1.2–2.0=large, and >2.0=very large. Precision of the estimate of observed effects was indicated with confidence limits (±95% CL).
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Results
Power output
All participants achieved at least 95% of the criterion score on first attempt. NaHCO3 improved power output in sprint 2 (P=0.011) and sprint 3 (P=0.021) in amateur participants. NaHCO3 had no effect on the power output of professional participants in any of the repeated sprints (P>0.05) (Figure 3). From sprint 1, mean power output was reduced in all subsequent sprints in both placebo and NaHCO3 in amateur (P<0.001) (Figure 4). No reduction in power output occurred in subsequent sprints from sprint 1 in professional participants (P>0.05) (Figure 3). Absolute difference between trials in professional and amateur was trivial (effect size = <0.01). Percentage difference in mean power output was greater amongst amateur players in sprint 2 (P=0.013; d=-0.183) and sprint 3 (P=0.025; d=-0.113) when compared to professional players
Blood lactate
Blood La concentrations for the placebo and NaHCO3 trials are summarized in Table 2. Blood La concentrations were similar in both trials at rest in professional (P=0.893; d=- 0.107) and amateur (P=0.080; d=0.045), although increased in both conditions pre-exercise but still remained similar in professional (P=0.176; d=-0.87) and amateur (P=0.849; d=- 0.09). Post-exercise blood La concentration was significantly greater in professional participants during NaHCO3 trial than placebo trial (P=0.011; d=-1.19) although no difference in the two conditions in amateur (P=0.093; d=-0.59). Additionally, the absolute difference from placebo to NaHCO3 was similar in both professional and amateur at rest (P=0.497), pre-exercise (0.250) and post-exercise (0.491).
Gastrointestinal discomfort
Stomachache and sickness feelings in both trials did not differ at rest in professional (P=0.317), amateur (P=0.317) or pre-exercise in professional (P=0.157) or amateur (P=0.083). Post-exercise gastrointestinal discomfort increased from rest in NaHCO3 trial in both professional and amateur participants (P=0.002), but not in placebo trial (P>0.05). The absolute difference in feelings of gastrointestinal discomfort between professional and amateur were no different when compared to rest (P=1.00), pre-exercise (P=0.298) or post-exercise (P=0.268) values.
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Discussion
The primary aim of this investigation was to compare the effects of sodium bicarbonate ingestion (NaHCO3) on anaerobic performance of professional and amateur soccer players during repeated-sprint bouts. Results have revealed that despite an improved mean power output of amateur participants in the mid-section of the repeated bouts (sprint 2 and 3 out of 5) in the test, NaHCO3 had no greater effect on amateur than professional participants throughout the testing protocol. Additionally, secondary findings from the study highlighted the failure of NaHCO3 to affect post-exercise blood La concentrations of amateur participants, whereas NaHCO3 increased the postexercise blood La concentrations of professional participants
As seen within the study, the mean power output of professional participants was not altered by NaHCO3 ingestion. The higher endogenous muscle buffer capacity of the professional participants within this study may provide a possible explanation to this observation. Indeed, muscle buffer capacity may be increased by 25% following five consecutive weeks of high-intensity interval training (3-sessions/week) [25]. Furthermore, elite athletes are known to possess greater muscle buffer capacities when compared to their amateur counterparts [25,26]. In accordance with the findings of this specific study, previous research also suggests a greater endogenous muscle buffer capacity of professional participants is more than likely responsible for the maintenance of mean power output throughout both NaHCO3 and placebo trials. Subsequently, it should be noted that this would not explain the conflicting reports of Bishop et al. [2] who, using a similar experimental protocol, reported an 8.5% reduction from sprint 1 to 5.
The lack of any ergogenic effect of NaHCO3 among professional participants suggests their greater endogenous buffering capacity blunted any performance enhancing effects of the NaHCO3 supplementation [16]. The improved performance amongst the amateur participants following the NaHCO3 supplementation highlights the positive buffering effect of acute supplementation on less trained individuals. Such findings are in agreement with previous data associating NaHCO3 with no performance effect in anaerobically trained professional athletes when compared to amateurs [2,13,14]. From a practical perspective, professional players who are recently returning to training (e.g. injured) may benefit from a NaHCO3 supplementation to maintain increased training intensity
Recent research has suggested that NaHCO3 is effective in its role of decreasing post-exercise La- [2,27]. Consistent with previous research [2,28] NaHCO3 failed to improve mean peak power output of both professional and amateur participants during the first sprint. It was suggested that a single 6-second sprint maybe too brief for the buffering mechanisms of NaHCO3 to be effective, however, repeated 6-second sprints may benefit through greater facilitation of H+ efflux from the muscle [28]. Similarly, it has been suggested that single short sprints with brief recovery (~17-seconds) may not allow adequate translocation of metabolites from the myoplasm [5]. Again, consistent with previous research [28] and supportive of recent suggestions that NaHCO3 is effective during longer exercise of ~1-minute for instance [12].
In contrast to previous findings [2] NaHCO3 failed to improve the performance of professional or amateur participants in sprints 4 or 5. Early research suggested the ergogenic effects of NaHCO3 are associated with large decrements in resting H+ [27] suggested maximal decrease in H+ occurs 60-90-minutes postingestion of 0.3g-1.kg-1 NaHCO3 however, NaHCO3 administered 60-minutes pre-exercise resulted in a 42% performance enhancement [28]. Latterly, recent research has suggested peak blood alkalosis can be expected ~120-150-minutes postingestion of 0.3g-1.kg-1 NaHCO3 [12].
It is possible the amateur participants began the exercise with suboptimal PCr stores following an intensive warm up (3 practice sprints and 10-second sprint) and only 5-minutes recovery; with PCr resynthesis reported to reach only 85.5±3.5% of resting levels during 6-minutes recovery following a 30-second sprint in amateur participants [29]. With strong correlations between percentage PCr resynthesis and percentage recovery of power output and maintenance of muscle power output [29] Professional athletes were better able to maintain power output during the repeated sprint than amateur, presumably because the rate of PCr resynthesis is known to be more rapid in professional athletes [30].
Interestingly, pre-exercise blood La concentrations were increased from rest in both professional and amateur participants, suggesting high rates of glycolysis during the warmup. Early studies suggested that La accumulation was the cause of fatigue during high-intensity exercise [31] meaning both professional and amateur participants began the exercise in an acutely relative fatigued state. Although recent reports reject this relationship, with new evidence suggesting reduced rates of PCr resynthesis is the main cause of reduced exercise performance during repeated sprints and not the acidosis resulting from La production [31].
Strong correlations exist between aerobic power and the maintenance of repeated-sprint performance [13]. The increased contribution of aerobic metabolism and reduced contribution of anaerobic glycolysis through repeated sprint repetition as reported by Gaitanos et al. [13] provides sufficient explanation of the current results; where the contribution of anaerobic glycolysis probably fell during sprints 4 and 5, offsetting the potential for performance enhancement by NaHCO3. Further, the probable lower aerobic power of the amateur participants meant they were unable to meet the increased contribution of aerobic metabolism in the latter sprints meaning they were unable to maintain mean power output.
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Perspective
Results within this investigation have revealed that despite an improved mean power output of amateur participants in the mid-section of the repeated bouts (sprint 2 and 3) test, NaHCO3 had no greater effect on amateur than professional participants throughout the testing protocol. This is in contrast to previous research who failed to show any performance improvement of professional or amateur players following NaHCO3 supplementation [2]. Furthermore, within this study professional players were better able to maintain power output during the repeated-sprint protocol than amateurs, presumably because the rate of PCr resynthesis is known to be more rapid in professional athletes [30]. From a practical perspective, the current investigation although unique in its concept suggests that amateur participants in soccer may benefit from NaHCO3 ingestion more than professional players
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Conclusion
It can be concluded from this particular investigation that despite no significant differences were found concerning the ergogenic effects of NaHCO3 among professional players, there was however a positive effect amongst the amateur soccer participants. Findings revealed how NaHCO3 may be used to create a positive buffering capacity during initial stages of repeated-sprint exercise bouts when supplemented amongst amateur participants or individuals with a less trained physical profile (e.g. recently injured, post-cessation of training). In addition, it was found that the increased levels of alkalosis failed to improve the mean power output of professional participants. Therefore, in conclusion, it may be suggested that amateur participants in soccer may benefit from NaHCO3 ingestion more than professional players.
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Acknowledgement
This manuscript is original and not previously published, nor is it being considered elsewhere until a decision is made as to its acceptability by the Editorial Review Board. There are no funding sources and are no conflicts of interest surrounding this scientific investigation
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Conflict of Interest
There are no conflicts of interest concerning this paper
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The Path to Regulated Immortality
5-9 June 2019 took place in Moscow the XVIII International readings in memory of N. F. Fedorov. Nikolai Russian Fedorov is an original Russian thinker, one of the representatives of the scientific and philosophical direction, called “Russian cosmism”. The pathos of his teachings is associated with the imperative of the evolutionary ascent of mankind, and the main task was formulated as “the resurrection of the fathers”, that is, the restoration of the forces of science of the genetic code of all previously lived on Earth generations of people with the aim of their full bodily reconstruction. Despite the mystical coloring and discussion of this idea, it has had a significant impact on philosophy and science in Russia. In particular, the follower of Fedorov was the founder of Russian cosmonautics Konstantin Tsiolkovsky, who came to the idea to explore outer space and create rockets for this purpose at a time when he realized that in the case of the project “resurrection of the fathers” reborn to a new life people will not be able to fit on the globe.
In our days, the idea of Fedorov takes on a special urgency. In the conditions of growth of technical potential of mankind, at expansion of the power of a civilization over the nature along with positive effects destructive tendencies increase also. Among them, such as the unexpected decline in the growth rate of the human population and the aging of the population (especially in developed countries), the rapid increase in mortality from “diseases of civilization”, mainly from cardiovascular diseases and cancer. Recently, a lot of talk is about the radical transformation of the human body using the latest achievements of biology and medicine, in particular by means of technological and genetic engineering (transhumanism project). Thus, the threat of “human death”, which Michel Foucault, Roland Barthes and other structuralist philosophers spoke about 50 years ago by means of metaphors, is becoming a reality today. it is a problem of direct, physical survival of man and humanity.
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Original Position
As part of the Readings at the Institute of philosophy of the Russian Academy of Sciences on June 6, 2019, I read the report “the Reality of individual immortality as a General revolutionary problem”. The main content of the report is that the passive and fatalistic attitude towards human death must now be replaced by an active and conscious impact on this process in the form of “regulated immortality”. In fact, it is a fight against aging, because aging is a slow dying. To make such statements allows me not only the status of a professional philosopher, but also a professional physician: more than 20 years I worked in clinical medicine as a specialist in anesthesiology and resuscitation. The sequence of arguments is as follows: A. Before proceeding to the discussion of practical approaches to regulated immortality, it is necessary to rethink a number of theoretical issues. It is necessary to look at the current situation from a higher, philosophical point of view and previously develop an updated understanding of such phenomena as immortality, life and the essence of living matter, man, the biosphere. This topic is devoted to the subsequent presentation, where the known facts are put in a different, unusual relationship. B. Human immortality should be understood as an opportunity to extend human life beyond what is now seen as natural, habitual and maximal. This means that we are talking about relative, but not absolute immortality. Only the World Universe can be absolutely immortal, and even then, some physicists deny this view.
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Essence of Life
Now, about life. The essence of life modern science connects with the ability to self-development and evolution. Its main property, as stated in encyclopedias and reference books, is growth-increasing complexity, diversity, strength. This is true, but such a statement is at least incomplete, since the ability to grow and have non-living systems: crystals, as noted by Herbert Spencer, grow, fire spreads, stars evolve.
The difference between living and non-living systems lies in another plane. Non-living systems are deprived of one quality, namely the internal ability to die-non-living systems cease to exist only when external resources disappear: crystals lose the environment of their growth, fire burns all its fuel, stars consume their composition. This can be called extinction or exhaustion, but it is not the death that living beings and systems die. The death of living beings is not so much the result of external as of internal processes. The conclusion is that die only live system. And from this point of view, life is the process of maintaining, extending life by moving away, moving away the moment of death.
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The Specificity of Man
After this conclusion, we look at the evolution in nature. Here we see that more complex and highly organized living species have a wider range of habitats, have a larger brain volume and, most importantly, are characterized by higher life expectancy than simpler and less organized species. According to this indicator, the human species is among the leaders, and among the primates it is an absolute and absolute leader: the life expectancy of a person is 2 times longer than the life expectancy of a chimpanzee.
In addition, people in the process of evolution there is a special period-aging, which is either not at all in the lower organized species or is very short. And aging, as mentioned before, is slow dying. Consequently, man has some resources that push death to a later period
What are these resources?
These resources are associated with a special way of life that is unique to humans. Let us dwell on this aspect. At first glance, humanity-is the same living species as all kinds of animals: it is, drinks, sleeps, multiplies, like other biological beings. But this is only at first glance, in fact, between man and animals there is a fundamental, unique difference: the specificity of man as a representative of his species is that since its inception (CRO-magnon man, formed 35-40 thousand years ago) and to the present historical moment, he remained and remains morphologically unchanged, although during this period has made enormous changes in the environment. Other living species can also be morphologically stable, but they maintain their stability by maintaining their ecological niche, that is, the immutability of their environment, while humans continuously and on an increasing scale recycle the environment.
Man achieves this through the use of technology, that is, the creation of artificial tools. As Benjamin Franklin once put it (and then repeated Karl Marx in capital), man is an animal that systematically uses tools. And-I will add-is no longer actually an animal.
Animals, too, use tools, but not artificial, as people, and natural-in the form of organs bodies, for example, in the form of fangs, claws, skins. In other words, animals exist at the expense of their morphology, and the animal world evolves at the expense of changing morphology of species. While man-I emphasize againis morphologically unchanged. As a result, humanity becomes homogeneous, and the human species is transformed into a race. However, the morphological dependence in humans does not disappear completely, but moves to a new level-from the level of the species to the level of the individual. That is morphologically changed not the species, as in animals, and the individual. This change is the process of aging, slow dying. Thus, the immortal human race existed and exists at the expense of mortality of a particular human individual.
This is what the situation looked like before, in the past history. But today the situation has changed-modern humanity is no longer immortal, it threatens to disappear from the results of its own activity, from the development of tools, from its superpowers. In this case, we are talking not only about the military threat, but also about new, recent threats-environmental (so to speak, external to humanity) and biotechnological (internal, in the form of the project of transhumanism). The cumulative result of these threats is the above-mentioned trend of depopulation of mankind by reducing the birth rate and increasing mortality from cardiovascular and cancer diseases.
What to do?
Some people hope for new scientific directions, synthetic biology and personalized genomic medicine, which are a variant of positive eugenics. However, if we correctly assess the latest data of evolutionary theory, both of these areas look hopeless and even dangerous. The fact is that the genetic mechanism in the body of animals and in the human body works differently. Consider this question in more detail.
Julian S. Huxley also claimed that genes do not directly determine the morphology (phenotype) of a living organism, but act indirectly, taking into account environmental factors (this effect is actually realized as natural selection, discovered by Charles Darwin). But the external environment of man is different from that of animals-it is not only natural, but also artificial, that is, represented by culture in its totality of phenomena, including tools. Therefore, the genes in humans are different than animals: famous geneticist Richard Lewontin argues that the human genotype is associated with human pinotepa much weaker than all other living species, and it is possible that some historical moment, this relationship has gained a fundamentally new character. This is quite acceptable, because the genome of animals serves their adaptation to the environment and therefore is reproduced along the line of change, whereas the human genome, which adapts nature to itself, should be oriented in the opposite direction-along the line of stabilization.
It follows that the manipulation of the human genome is unlikely to contribute to the cure of diseases, as stated by the representatives of “genomic medicine”; most likely, on the contrary, they will lead to genetic disorders up to the occurrence of deformities and disorders in human heredity
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New Perspective
Where, then, to seek salvation from the natural and technical degeneration that threatens modern humanity? Is it really necessary to stop the technical development, to stop using the latest tools and return to the primitive state, as suggested by the supporters of the “green civilization”, calling for an end to torment the single organism of the Earth-Gays, in the words of James Lovelock?
just need the full power of modern humanity to be deployed to a new purpose-to extend the life of modern man, his “adjustable immortality,” to fight with death in the spirit of the main ideas of Nikolai Fyodorov. We know that there is no absolute immortality. All non-living and living systems, sooner or later they break, degrade, fall apart. However, there is one organism that is constantly not only alive, but also young due to the continuous process of renewal. This organism-the biosphere, which does not borrow resources from the environment, like all other living systems, and takes them from within, from itself through constant recombination and restructuring of its composition. But the biosphere does it only for itself, in a single copy. To achieve lasting immortality of the human species and a significant extension of the life of the human individual, it is only necessary to apply such a restructuring in relation to each person. And for this it is necessary to understand the laws of the process of renewal in the biosphere and transfer them to humanity.
There is no doubt that this will require great social changes, changes in the entire system of modern medicine. At the same time, the most difficult is the preliminary process of radical transformation of the thinking of doctors and all medical workers. The task of “regulated immortality” requires a fundamentally different worldview than the one that prevails today: medicine from disease control technology should become a technology for combating aging, and at a much earlier stage than it is understood today. It must be a struggle for youth-a struggle to prolong life-a struggle against death. It is extremely difficult, but it is quite possible. Further research in the field of philosophy and philosophically meaningful medicine will demonstrate exactly how this goal should be achieved. These are the prospects that are outlined in the scientific and philosophical understanding of the main idea of Nikolai Fedorov.
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Biochemical Response Comparisons of a Competitive Microcycle Vs. Congested Fixture Periods in Elite Level European Champions League Soccer Players
The aim of this investigation was to analyses differences between post-game biochemical responses in congested (CP, when two consecutive games were played within 4 days or less) vs. non-congested (NCP, when two consecutive games were played within 5 days or more) periods. Assessment of creatine kinase (CK) and salivary cortisol (sCort) and immunoglobulin-A (sIgA) were performed across 12 separate training macrocycles of 14 competitive professional matches. The NCP analysis revealed significantly higher CK values from one day after match day (MD+1) than values found in MD and MD+2 (Effect size (ES): 0.82-1.13, p<0.0033). The CP analysis revealed no significant changes in CK values between MD, MD+1 and MD+2. Higher values on MD+2 in CP vs. NCP were also revealed (ES:0.27, p<0.05). No significant changes in sCort and sIgA were observed. Congested competitive periods induce significant biochemical changes revealed amongst elite soccer players that should influence their preparation and management
Keywords: Cortisol; Creatine kinase; Immune function; Football; Fixture congestion
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Introduction
Across the course of an elite soccer season, the continual training, playing demands and additional stressors imposed upon players can lead to significant changes in haematological profiles [1]. Soccer is characterized by high intensity intermittent activity patterns. Players perform numerous intensive accelerations and decelerations in conjunction with high-intensity activities including sprinting, jumping, kicking and changing direction [2,3]. Such activity can cause muscle fiber damage. In the literature, CK is commonly used to examine exercise severity and exercise-induced muscle damage [4-6]. A soccer-game impose on players to repeat high numbers of very intensive accelerations and decelerations [7] that involve both concentric and eccentric contractions of the lower limb muscles, which may induce micro damages and structure changes to the muscles [8,9]. CK values have been shown to increase between 24-48h post-competitive soccer play, and to return to baseline approximately 72h or more [10-13], however, interestingly the variation across a typical competitive week of CK values linked to training load (TL) distribution have to date not been investigated. The presence of muscle damage after a soccer match could potentially impair subsequent training and competition performances, however further research is needed.
In addition, with CK analysis, and according to previous literature in this area, salivary cortisol (sCort) is another biochemical marker that is highly responsive to exercise and is directly related to the catabolic activity [14]. Cortisol has recently been suggested as a relevant indicator to monitor recovery in soccer, in response to the induced stress [15]. Induced stress by exercise is associated with the stimulation of the hypothalamic corticotropin-releasing hormone, pituitary adrenocorticotropic hormone release and the subsequent production of sCort [16,17]. Increased levels of sCort as a result of accumulated stress-inducement, can negatively impact mucosal immunity, subsequently increasing the risk of illness [18]. Based on the fact elite soccer players are faced with increased congested fixture schedules at domestic, European and International levels, increasing the knowledge of biochemical fatigue and recovery kinetics may assist in the TL management during congested periods (CP) in order to avoid injury and optimizing physical recovery and/or performance. Recent findings have suggested that congested fixture periods are associated with perturbations in mucosal immunity [19] and an elevation of injury incidence [18,20,21] observed a greater reduction in mean relative salivary SIgA concentration following an intensive competition period (nine games in eight weeks) compared with a less competitive period in rugby union (two games in four weeks) (29% vs 9% decrease in salivary SIgA). Moreover, the decrease in absolute SIgA concentrations were associated with a corresponding increase in sCort [18]. Furthermore, Mohr et al. [22] observed how playing three matches per week increased muscle damage, inflammatory and oxidative stress responses. Moreover, it was observed that the second match played after three days of recovery induced higher muscle damage and inflammation vs. the third game played after four days of recovery [22]. This maybe as a result to lingering muscle damage from first game causing added CK release as in poor muscular recovery. It might therefore be suggested that differences exist between periods of matches with three days in between, (e.g. CP) [23] and periods of matches with four days and more in between (non-congested period, NCP). Changes in these parameters have been previously associated to fatigue related to match play [12,15]. However, to our knowledge, no previous investigation has analysed the differences between CP vs. NCP regarding the physical and biochemical incidences of match play at any level, especially in elite level soccer players. Therefore, the novel and unique aim of the current investigation was to explore the evolution of CK and sCort during the in-season competitive phase, and analyses differences between congested vs. non-congested competitive phases in relation to player’s post-match fatigue kinetics.
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Methods
Experimental approach to the problem
To explore the evolution of CK and sCort during the in-season competitive phase, and analyse differences between CP vs. NCP in relation to player’s post-match fatigue kinetics, all testing procedures were performed across the first phase of the 2016- 2017 season as to ensure no significant changes in physical status or fatigue was apparent and to maintain consistency of testing and sample collection [24]. The data set obtained, included 12 separate training macrocycles, inclusive of 14 competitive matches between August and October. The CP (n=5 macrocycles) was determined as a macrocycle including 2 competitive matches within 4 days or less. During this period samples were collected on match day (MD), 24h (MD+1) and 48h post-match (MD+2). The NCP (n=7 macrocycles) was determined as a microcycle including 2 competitive matches within 5 days or more. During NCP, samples were collected four, three and two days before the match (MD-4, MD-3 and MD-2, respectively), and on MD, MD+1 and MD+2. Although a total number of 59 training sessions were during the protocol period, only 28 training sessions were included in the procedure analysis, with a total number of 535 observations, to ensure a design internal validity and avoid sample-collecting bias.
Subjects
Twenty-three (n=23) elite male professional soccer players participated in this investigation. Players participating within this study were at the time competing at the elite level of European soccer within the UEFA Champions League and were recognized as the most successful domestic team in their national league. At the initiation of the study, players involved had a mean±SD age of 24.4±4.1 (range: 18 to 34) years, stature of 182.5±2.9 (168 to 195) cm, and body mass of 77.8±5.4 kg (61.2 to 93.5) kg. All participants had been playing soccer for 8 years or more and all but four of them were competing for their respective international team. Participants were informed that they were free to withdraw their individual data from the study at any time. Informed consent was obtained from all individual participants included in the study. The procedure performed in the present study was in accordance with the ethical standards of the institutional committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards
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Procedure
Throughout the investigation, players were allowed to consume commercially available isotonic sports drinks ad libitum during the training sessions and were also instructed to maintain normal daily food and water intake and CHO sport drinks during training when required. No additional dietary interventions were undertaken. Only data from players who met the following inclusion criteria similar to Coelho et al. [24] was assessed and included within the study (n=23). The established inclusion criteria required players had to have a minimum participation of 75min per game, not injured or ill on the collecting day or during the match and participated to the collective training sessions throughout the course of the macrocycle. If a player was injured, at any point through the macrocycle, their data for that period was excluded from the assessment until they returned to normal collective training with the rest of the group to play the official matches. The mean number of games played per player was 5.56 (range [1-11]). All blood sample collection was performed within the lab facilities of the club pre-training session. Trewin et al. [25] recently reviewed the influence of situational and environmental factors on physical outputs in soccer match-play. It was concluded from the findings that environmental factors influence the variability in match-running performance from match-to-match. As a result, taking the present design into account, many confounding variables may affect the data. Within this study all training and competitive match play was performed on natural turf pitches; the playing system adopted by the team remained in a 4-2-3-1 formation between the congested vs the non-congested fixtures.
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Training Load Assessment
Across the training sessions for the investigation, each individual player’s motion pathways were tracked using a 10 Hertz GPS device (Viper, Statsport, Ireland). Research has shown this system to be a valid and reliable marker of assessment for monitoring team player’s movement demands [26]. For the purpose of this study, absolute (in meters) and relative (in meters per minute) total distance covered (TDC), high-speed running (HSR: >19.8 km.h-1) and absolute (in number) and relative (in number per minute) Accel (> 1 ms-2), Decel (< -1 ms-2)and sprints (> 24.4 kmh-1). The total values taken from the complete training sessions were used for analysis. The players wore the same GPS unit for each training session in order to keep the validity of the data.
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Creatine Kinase Assessment
For the enzymatic measurement of plasma CK concentration, 32μL capillary blood samples were obtained from the fingertip of the index finger of the selected players pre-training and recovery days throughout the course of the investigation. The fingertip was cleaned with 95% ethanol and dried with cotton wool to remove excess liquid; an automatic lancet device was used to draw blood before a heparinized capillary tube (Reflotron®, catalog No. 955053202) was used to collect the sample. The capillary blood sample was then immediately placed onto a CK test strip (Reflotron®, catalog No. 1126695) through the use of a pipette and analyzed via the Boehringer Mannheim Reflotron Analyzer®.
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Saliva Sampling
Resting saliva samples were obtained pre-breakfast throughout the study period on each training and recovery day. Free days away from the training facility were not measured due to logistics. All participants rinsed their mouth with water 10 min prior to saliva collection. Unstimulated, whole saliva was collected using an IPRO oral fluid collector (OFC, IPRO Interactive, Wallingford, UK). Participants rested one OFC swab on top of their tongue and closed their mouth to stimulate the saliva release. Upon 0.5mL level of saliva being absorbed by the OFC, an indicator line of the swab stem turned bright blue at which point the OFC was removed from the participant’s mouth and placed into a 3mL buffer solution for analysis. Upon required saliva being taken, samples were then passed immediately to a test administrator and analysed in order to determine the sCort and the sIgA concentration using an IPRO Reader (Ipro Interactive, Wallingford, UK). This method has previously been validated for the determination of these two hormones in the sporting environment [27-28].
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Statistical analysis
Variables distribution was first tested with the Shapiro- Wilk normality test. When significant, a one-way analyse of variance (ANOVA) with repeated measure was used to examine the differences in the biochemical markers between days. Then t-test paired comparisons were used to point the differences. When non-significant, a non-parametric Friedman test was used, and then non-parametric paired Wilcoxon tests were assessed to point the differences. T-test paired comparisons and non-parametric Wilcoxon tests were also used to determine the differences between congested and non-congested weeks. Bonferroni’s correction was applied. The level of significance was set at p<0.05 for congested vs. non-congested comparisons and at p<0.0033 for days-to-days comparisons. For all analysis, the effect size (ES) was calculated and evaluated as small: < 0.50, moderate: 0.50-0.80, or large: > 0.80, as described by Cohen [29]. Coefficient of variation (CV) was also calculated from the ratio standard deviation (sd)/mean.
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Results
MD-4, MD-3, MD-2 and MD-1 = four, three, two and one days before the match, respectively MD = match day; MD+1, MD+2 = one and two days after the match, respectively. CK = creatine kinase sCort = salivary cortisol TDC = total distance covered HSR = High-speed running (> km.h-1) NA = non-assessed * Higher than the same measure during non-congested week (p<0.05)
Data were presented as mean±sd throughout. Biochemical marker and training load changes across the weeks are presented in Table 1. During the typical NCP, CK values in MD+1 (CV: 45%) were significantly higher than values in MD-4 (ES: 1.11, CV: 37%), MD-2 (ES: 0.89, CV: 68%), MD (ES: 0.82, CV: 64%) and MD+2 (ES: 0.93, CV: 63%) (p<0.01) while CK values reported in MD-3 (CV: 65%) were higher than those reported in MD+2 (ES: 0.82, p<0.01) and MD-4 (0.81, p=0.05) (Figure 1). sCort values assessed throughout the week presented no significant changes (CV: 72-141%) (Figure 2). During the CP, no significant differences were observed among the day’s neither in CK, sCort and sIgA. When comparing CP and NCP, higher CK values were observed in MD+2 in CP (vs. MD+2 in NCP, ES: 0.27, p<0.01) (Figure 3). No differences between CP and NCP were observed in sCort and sIgA values. TDC (ES: 1.09), HSR (ES: 0.35) and accelerations (ES: 0.36) metrics all showed higher values in MD-1 during CP vs. NCP (p<0.05) (Table 1).
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Discussion
The aim of the present study was to compare the variation of CK, sIgA and sCort responses during congested and noncongested periods in elite level soccer players. CK is well reported within literature as an indicator of exercise severity and exerciseinduced muscle damage [6,30] of which post-match increase was reported to be higher in soccer compared to other team sports like basketball, volleyball and handball [31]. Furthermore, CK, Cort and IgA were recently suggested as relevant indicators to monitor training load in elite soccer players [19,22,15]. The present study demonstrated that MD+1 (296±183 U.L-1) was the day of the week with the highest CK values (similar to MD-3 (283±185 U.L-1)) (Figure 1). This would highlight how muscular fatigue related to TL was at its highest point 24h following the match and three days before the match. Korean soccer players reported higher training-related perceived exertion (RPE) on MD-4 during the competitive season [32]. Similar high RPE were reported on MD-4 and MD-5 in young soccer players, and lower RPE in MD-3 and MD-2 (rest in MD-1). The elevated values of HSR (0.84±0.80 m.min-1) and number of accelerations (46.3±15.4) observed in MD-4 in comparisons to all other days, in the present investigation, confirmed that the analysed team followed the same microcycle periodization approach. Recent literature in the area of training specificity and tapering have highlighted similar tapering approaches, suggesting reducing TL as MD approaches to relieve fatigue and increase freshness [33-35]. Recently, it was reported amongst English Premier League soccer players that only MD-1 revealed a significantly reduced tapering effect and TL across a training microcycle when compared with other training days [33]. Additionally, Owen et al. [35] revealed significant differences in physical outputs were found between MD-2, MD-3 and MD-4 highlighting a structured periodized tapered approach (p<0.05). Furthermore, it was suggested that practitioners can maintain a uniformed and structured TL mesocycle whilst inducing variation of the physical outputs during the microcycle phase. However, it did not involve congested fixture periods in this study.
Days following the match revealed CK values were elevated MD+1 (296 ± 183 U.L-1 vs. MD: 183±118 U.L-1, p<0.003) returning to baseline on MD+2 (176±111 U.L-1). According to previous research, increased levels of CK are commonly observed 24h post-match, however, further analysis reveals how CK values generally remain elevated at least 48h after the match (2,17,22,24,39,43) which is contrary to our observations. Prematch measures of CK (~183 U.L-1) were similar to what was previously reported in elite Greek soccer (~150 U.L-1) (22), 2nd and 3rd Portuguese divisions (~180-200 U.L-1) [10,36] French Ligue 1 players (~230 U.L-1) [37] values reported in young Italian Serie A (~310 U.L-1) [12] and in Premier League under-21 players (~343 U.L-1) [13]. Subsequently, results in the current study revealed lower values on MD+1 (~296 U.L- 1) compared to all previous reports (from ~600 to ~1411 U.L-1), and likewise in MD+2 (~176 U.L-1 in ours vs. ~500 to ~900 U.L-1) [10-13,36,37] although only one investigation did not report significant differences between baseline and MD+2 values [37]. Even though CK is known to present high individual and between-match variability (43) it might be suggested that the differences observed within studies were related to the match-related physical and psychological strain. Indeed, it has been recently reported that CK values 24h following the match are associated with the physical activity performed during the match [38,39]. Interestingly, the most marked increase in CK values post-exercise was reported in less well-trained subjects [40] and with lesser levels of lower-limb strength in elite level soccer players [6]. Taking these findings into consideration, it might also be suggested that the elite level players used in the present investigation were higher trained than those from compared literature. Furthermore, the investigated matches occurred at the beginning of the season when players just finished the high-TL pre-season period and were supposed to be at their greater shape [41,42] and subsequent lower biochemical markers related to fatigue [43]. It is therefore possible that the matches in the present investigation did not represent a much higher muscular stimulus than the usual training load of these players. This may therefore suggest that if teams and players are trained at a higher level of stimulus, then they may be able to recover from game with greater efficiency.
No evolutions were found in sCort concentrations between pre and post-match assessments (Figure 2). Recently, Souglis et al. [31] reported an increased level of blood cortisol immediately after elite team sports games including soccer, which returned to pre-match values 13 hours after the match. Also, Moreira et al. [16] observed a trend to increase in sCort between pre-and post-match measures in male professional players, but with no significant differences. These results were in accordance with those from the present investigation, which its first postmatch measure was only made 24h after the end of the game. Within all observations combines, it seems that sCort might increase immediately after a soccer game, but values might have returned to baseline the day after. Recently, one study reported decreased level of blood cortisol 24h and 48h after a soccer match, compared to pre-match values in young soccer players [15]. In this particular investigation, the elevated pre-match levels may have been caused by non-exercise stress, for example environmental and psychological stress can modulate cortisol [44].
The second findings from the present investigation was that CK, and neither sCort nor sIgA, was affected by a congested calendar of competitive matches. Indeed, the levels of CK assessed in MD+2 from the CP (206±116 U.L-1) were significantly higher than levels assessed in MD+2 from the NCP (176±111 U.L-1, p<0.05, ES: 0.27). Furthermore, the differences observed, with MD+1 being higher than MD+2 in NCP, were not anymore observed in the CP. CP have been associated with unchanged physical and technical performance during elite soccer matches, even when periods were prolonged for several months [45,46]. However, the literature reported an increase in injury occurrence in association with fixtures congestion [21,47]. Recently, Mohr et al. [22] reported higher CK responses when playing two matches in three days, than in four. The present report gave further and novel information that showed the impact of playing two matches in three to four days (vs. five or more) on muscle damages, which, as a supposed induced cumulated fatigue, were more elevated 48h after a congested fixture than when playing one match in a week with sufficient recovery. The levels of sCort and sIgA remained statistically unchanged in both CP and NCP. However, it was interesting to observe that sIgA values increased from MD+1 (292±157μg.mL-1) to MD+2 (333±243μg mL-1) during NCP and decreased (304±193 to 266±163μg. mL-1, respectively) during CP, even though not significantly (p>0.0033). Moreira et al. [49] recently reported a decrease in salivary immune-endocrine markers, like sIgA and testosterone, from the first match of a congested soccer tournament to the last one, in young elite players. They also reported no statistical variations in sCort across the tournament [50]. Earlier, Mortatti et al. [48] also reported no variations in sCort across a period of six matches in 20 days in young soccer players, and a decrease in sIgA in second and last matches. All together, these findings might suggest that a congested competitive calendar in elite soccer players induces fatigue related to muscle damage, mucosal immunity, and anabolism activity, but not hypothalamicpituitary- adrenal axis responses. A limitation associated with the current study is that testosterone was not measured, as such the testosterone: cortisol ratio could not be calculated. This ratio is of interest as it has been reported as an indicator of homeostasis between anabolic and catabolic process in the body [50,51]. Furthermore, the testosterone: cortisol ratio has been associated with overtraining in team sport athletes [14] and could be used to monitor stress and recovery state in soccer [38,52]. Interestingly, findings from the current study reveal how the there was significantly higher training load on MD-1 during CP when compared with the NCP. This finding may be as a result of increased freshness and the reduced training load of MD-2 playing a subconscious role on the application and motivation to train at a greater intensity. Although this is difficult to predict and requires further investigation in the future, the technical staff may also have increased the content somewhat as a way of ensuring the players are prepared for the additional fixture post- a low loading day. Furthermore, it should be highlighted that this increase in workload pre-game may have played a role in the accumulated fatigue in the CP even though the effect size in difference were small for both HSR and accelerations (0.34- 0.36). Even though the workload was slightly increased across the CP vs. NCP the biochemical stress observed through prematch CK assessment was the same. These observations further indicate the need from the technical staff to plan and understand the impact CP plays on the fatigue and state of the player in order to manipulate accordingly training workload to increase performance [53].
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Limitations
Ensuring large subject participation within elite level soccer research is extremely difficult. The ability to meet subject inclusion criteria is often challenging due numerous external influences such as suspensions, team selections, injury and illness across an in-season period of testing. Therefore, with respect to this investigation, future research should look to draw from baseline data across more than one team at different levels to show how they can be compared across. Ensuring a larger subject pool in addition to increasing the sampling period and getting a same number of matches collected within the periods in order to add greater strength to the association demonstrated across CP vs. NCP would be beneficial however, although understanding this as a priority, unfortunately this is not always possible when conducting scientific studies at the elite level of a sporting organization. Furthermore, it may be beneficial in future research to examine a similar experimentation method across a range of competitive levels (i.e. professional, youth and amateur) as a way of determining key differences at varying playing levels and allow the findings of this to increase knowledge of changes of differing proficiencies. It should be highlighted that a limitation of the study was not including a control group. This is something that future research should try and include, however based on the fact that the study group was a group of elite professional soccer players, the resources available were allocated for the primary professional team.
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Conclusion
The purpose of this investigation was to explore the evolution of biochemical responses of CK, salivary IgA and cortisol during the in-season competitive phase, and analyse the various responses associated with congested vs. non-congested competitive phases in relation to player’s post-match fatigue kinetics. The findings of this study may further enhance the capability of individuals involved within the preparation of elite players to manage specific congested or non-congested phases of the competitive season to produce improved decisions as the players in the present study showed impairments in postmatch CK levels during CP. Conclusions drawn from the current investigation suggests that congested competitive periods at the elite level of the game induce significant changes in biochemical stress responses (i.e. muscle damage). As a result of these findings individuals involved with the physical preparation of such players should understand the demands imposed on players in congested fixture periods and adequately adjust preparation and squad rotation accordingly.
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Practical applications
At the elite level of the game professional soccer training and competitive match play induces a significant chronic stress on the body, which influences the biochemical responses of players [1]. Such continued demands and fluctuating physiological changes predispose players to health, wellbeing and performance risks based on the high intensity and energy expending nature of the sport. This novel investigation is the first study to have examined the relationship of biochemical responses based on assessment of salivary cortisol and CK across congested and non-congested periods at the elite level of the game. Based on the data collected in the current study, it is recommended manipulation of the training intensity, recovery strategies and the intelligent integration of squad rotation processes be present as a way of maximising performance whilst reducing the risk of chronic fatigue and injury risk. This study may open up the discussion area of additional ways to include monitoring and assessment of players through congested periods of the competitive season and be considered as one of many factors in injury prevention. Greater focus should be placed on the training intensity and volume over a longer-term, especially approaching congested periods of the season with the aim of ensuring the potential reduction of fatigue that may lead to overuse injuries. Therefore, regularly monitoring biochemical changes (i.e. CK) within the preparation period and across the competitive season to try and ensure optimal player preparation state is something that should be highly considered.
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Prescribing Trends in the outpatient Department in A Rural Hospital in Bangladesh
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This study was carried out to find the medicine-prescribing pattern in outpatient department of Medicine, Surgery, Gynecology and Obstetrics in government and private hospitals in Faridpur city, Bangladesh. To see the patterns of prescriptions of 250 patients were collected over a period of three months and analyzed for the average number of drugs per prescription, number of drugs prescribed per prescription, most common diagnosis, most commonly prescribed antibiotics, most commonly prescribed groups of drugs, percentage of drugs prescribed by generic names, percentage of drugs prescribed from Essential Drug List (EDL) of Bangladesh. The average number of medicines per prescription was 4.14 and 48.8% patients were prescribed up to 3 medicines. Only 37.I6% drugs were prescribed from EDL of Bangladesh. Percentage of encounters with an antiulcer ant, a NSAID and a multivitamin & mineral prescribed were 29.25%, 18.15% and 9.84% respectively.
It was evident that 73.6% [184] of the prescriptions contained antibiotics. Cefixim and ciprofloxacin were found to be the most preferred antibiotics in physician’s prescriptions survey. The diseases, which were seen in the studies, include cough, typhoid, diarrhea, nausea, chronic UTI, RTI, fever and rhinitis. The result of the present survey indicates that antibiotics are widely and inappropriately practiced without following standard guidelines. This is an alarming condition in the health sector of Bangladesh and therefore, the respective authority should take necessary steps to minimize the harmful effects of antibiotics.
Keywords: Prescribing pattern; Essential drug; Antibiotic; Faridpur city; Rational use of drug
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Introduction
Knowledge of risk perceptions has demonstrated to be vitally important in understanding how individuals and societies manage the risks of daily life. In medicine perceptions of drug risks are probably to influence patients’ treatment decisions, their compliance with treatment regimens, their views on the acceptability of adverse reactions and the drugs that cause them, and their attitudes toward government regulation of medications. Understanding perceptions is a prerequisite for designing better communication materials for patients and the general population [1]. The drug use process can be separated into four fundamental components, namely: prescribing, dispensing, administration/ uptake and outcome (efficacy/safety) [2]. The evaluation of medication use is vital for clinical, educational and economic purposes [3]. Monitoring of prescriptions and drug utilization study could distinguish the related problems and give feed backs to the prescriber in order to make awareness for the rational use of drugs [4]. It is therefore necessary to define the prescribing pattern and target the irrational prescribing habit for sending a remedial message [5]. Therefore, the present study has been undertaken to observe the prescribing patterns of Antibiotics and the usage of these agents in different types of patients with different types of diseases in Faridpur City of Bangladesh
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Materials and Methods
Study area, duration of research project and data collection process. A cross-sectional study was conducted in order to evaluate prescription pattern of antibiotic drugs in patients with various infectious diseases. On the basis of inclusion and exclusion criteria prescriptions were collected from all patients (both male and female) attended the outpatient (OPD) at government and private hospitals in Faridpur city. The study was carried out over 90 days period of April 15th, 2018 to July 15th, 2018. A total of 250 patients were included in the study. New patients attending the outpatient department of Medicine, Surgery and Gynecology and Obstetrics in government and private hospitals in Faridpur city during the study period were considered for analysis. Follow up visits during the study period were included and were counted as separate visits. Patients visiting the emergency department or who got admitted during OPD visit were not included in the study. The average number of drugs per prescription, number of drugs prescribed per prescription, most common diagnosis, most commonly prescribed antibiotics, most commonly prescribed groups of drugs, percentage of drugs prescribed by generic names, Percentage of prescriptions with injectable preparations., percentage of drugs prescribed from Essential Drug List (EDL) of Bangladesh, The data was expressed as percentage, mean and total numbers
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Results
Total number of 250 medical case records were collected, scrutinized and analyzed for drug prescription. The majority of the patients were male 157 (62.80%) and 93 (37.2%) patients were female with 14.80% of them aged between 18-50 years Table 1.
Among 250 patients, we found that majority of the patients were victim of gastrointestinal tract infection (51.2%). Other patients suffered from orthopedic disorder (26.0%), respiratory tract infection (8.0%), ENT infections (8.0%) and urinary tract infection (6.8%) respectively. These data are shown in Table 2.
In this research project, A total of 1036 individual drugs were prescribed for 250 drug encounters, giving an average of 4.14. The range of drugs per encounter varied from 1-7. There was not a single prescription wherein no drug was prescribed. Moreover, very few drugs were prescribed by generic name (1.93%). These data are represented in Table 3
Total number of drugs in our prescription was 1036. Among these, the most prescribed drugs were of acid related preparations (29.25%). Then the second most prescribed drugs were antibiotics (28.09%) Table 4.
At least one antibiotic was prescribed in 184 (73.6%) of the 250 encounters and 77 prescription contain single antibiotic with other drugs. The most prescribed antibiotic was of cefixime 67 (23.02%). Then the second most prescribed antibiotics ciprofloxacin (17.87%) Table 5.
Among 1036 drugs only twenty drugs (1.93%) were prescribed by generic names. It was also seen that out of 250 prescriptions only 385 drugs (37.16%) were prescribed from the EDL of Bangladesh Table 6.
The present study also shown that omeprazole 58% and multivitamin with mineral 52% were prescribed among essential and non-essential drugs. The five most commonly prescribed drugs, which were included within or excluded from the EDL [6] of Bangladesh Table 7.
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Discussion
A prescription that is given by a doctor or physician is taken as an indication of the doctor or physician’s attitude towards the disease and the role of drugs in its treatment [7]. The central priority of health care system is providing the right medicine to the right people at the right time [8]. The source of data in these study 250 medical cases was collected and the percentages of male and female patients were 62.80% and 37.20%. Most of the patients were above 50 years old. Similar results were obtained from the previous study, which was conducted by Khan et al. [9]. This prospective study was conducted on 4800 patients who visited the OPD and IPD of ENT department of Teerthanker Mahaveer Medical Hospital and Research Centre of North India. Their result indicated that higher percentage of male person suffering from different infections.
We observed that in the data of this research project, most of the patients were suffering from gastrointestinal disorders (51.20%) and orthopedic disorder (26.00%). It is important to note that drugs should be prescribed in their generic names to avoid confusion. In this study the average number of prescribed drugs were 4.14 and drugs were prescribed by generic name is 1.93%. It also showed that 80.08% patients were given three or more drugs. The variation in results may be due to difference in characteristics of health care delivery system, morbidity and mortality characteristics in the population. Since, WHO has recommended that average number of drugs per prescription should be 2.0 [10], the results of the study reflect polypharmacy which may lead to adverse drug reactions, increase the risk of drug interactions, dispensing errors, decrease adherence to drug regimens and unnecessary drug expenses.
Antiulcerant drugs, 207 prescriptions (69%) were found and the omeprazole [148 prescriptions (49%)] was the most commonly prescribed of this class. The present observation remarked that 73.60% prescription contained antibiotic drugs along with other drugs. Among 41.85% prescription contained single antibiotic drug and 58.15% contained two antibiotic drugs. No prescription contained more than two antibiotic drugs. Prescriptions among which antibiotics that lie under Cefixime, Ciprofloxacin were most commonly prescribed. Similar results were obtained from the previous study, in Iran (61.9%) [11]. In another study by Biswas et al revealed that mostly prescribed drugs were also antibiotics (49.22%) [12]. According to WHO 15- 25% of prescriptions with antibiotics are expected, where infectious diseases are more prevalent [13]. In a 3rd world developing country like Bangladesh, prevalence of infectious diseases is higher than the developed countries. That is why; in this study the antibiotic utilization rate was higher than that of developed countries. However, this result does not indicate that the prescription pattern was better than in other countries.
The WHO recommended target for injection exposure is 10% or less [14]. In this study, the percentage of prescription with an injection encountered was 2.32% which is less than in Nepal (3.1%) [15], Zimbabwe (13%) [16] and India (13.6%) [17]. so, the observed proportion of injectable drugs prescribed may be considered acceptable according to WHO recommendations. Minimum use of injections is preferred, and this reduces the risk of infection through parenteral route and cost incurred in therapy [15]. It also showed that out of 250 prescriptions 102 (40.80%) had at least one multivitamin and multimineral prescribed which was not enlisted in EDL. The justification for this practice is not clear. However, some patients and doctors believe that the multivitamin supplement may induce or enhance the patient’s appetite or relief from weakness. In this study, the percentage of drugs prescribed from EDL of Bangladesh was 43.16%. The possible reason for this lower value could be the prescribers lacking the understanding the importance of essential drug concept. The low rate of prescribing from EDL of Bangladesh may be also contributed by excessive use of multivitamin and multiminera, antiulcerant (Ranitidine) and antihistamine [Cetirizine HCl], which are not enlisted in EDL of Bangladesh. So that the higher percentage of non-essential drugs in this study is responsible for inappropriate use of medicines.
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Conclusion
Physician is often considered to be God and therefore when a doctor gives an antibiotic to a patient, it is taken without question. However, a doctor is often pressurized to give antibiotics for a variety of reasons. It could be due to the pressure of making a patient well as soon as possible, or the fear of losing patients to another doctor. Patients often demand powerful treatments, and then there is the industry pressure and incentive schemes, and, most importantly, the doctor`s own clinical judgment skills. To be fair, many doctors work with inadequate and unreliable investigational facilities. This may promote the use of combination antibiotics and defensive medicine. From the result of this study, it can be concluded that inappropriate drug prescribing, inappropriate use of drugs and irrational prescribing of antibiotic are major problems. Therefore, there is a need strict enforcement and adherence to existing regulations regarding antibiotic practices. To overcome these problems, the drugs control authorities should be better equipped and more vigilant to cope with the present situation. Health professionals and drug manufacturers should be more committed in order to achieve the goals of the National Drug Policy of Bangladesh.
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Efficacy Aloe Vera In Treatment: A Literature Review
Aloe vera is a medical which has many benefits that is used in medicine to several problems in the health of human body. Aim: Conducting a literature review study related to efficacy of aloe vera which is used for treatment in healing with body health problems. Methods: Article searching start on date 10-25 August 2018 via the CINAHL database, ResearchGate, Pubmed, and ScienceDirect which is used keyword effect AND aloe vera then, selection based on inclusion criteria in the article and after getting results inclusion criteria is done critical appraisal so that relevant articles number of 9 articles. Results: Of the 9 articles, there were 8 researching articles indicate that using aloe vera has benefits in accelerating the process of wound healing and decrease pain intensity heal burns. There is one researching article indicate date aloe vera can also be used as therapy or treatment in melasma patients with fix function skin pigment. Conclusion: Utilization of treatment using aloe vera proven to accelerate the process of wound healing and decrease some health problems of pain intensity.
Keywords: Leaf aloe vera; Efficacy; Treatment
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Introduction
Medical plants are the best source for getting treatment from various disease [1]. Using of natural substances with therapeutic properties has been used since ancient times [2]. At present, a number of prescribed drugs comes from plants [3]. Aloe vera has been used for medical purpose in several cultures for a thousand of years Egypt, India, Mexico, Japan and China [4]. This plant has triangular leaves. Fleshy with jagged edges, yellow tubular flowers and fruits which contain a lot of seeds. Every leaf consults of 3 clear get which of containing 99% water and the other are made of glucomannan, amino acid, lipids, sterols, vitamins, and middle lays of latex which of bitter yellow sap and contains anthraquinone, glycosides, and thick out layer of is 15-20 cells referred to as peel that has a protective function, synthesize carbohyidrates and proteins. Inside the skin is a collection of blood vessels responsible to transport substances such as water (xylem) and starch (phloem) [4]. Various aloe vera extracts are mate to be easy is used in medicine to humans like gel, oil, juice, and tablets. In several researches in-vitro indicate that aloe vera leaves showed contain more than 75 nutrients and 200 active compounds, including 20 minerals, 18 amino acids and 12 vitamins. Also contain the most important components which is needed by the human body [5] Aloe vera has vitamins A, B1, B2, B6, B12, C and E. Aloe vera has a high enzyme content (for about 92 enzyme), which makes it a rare source and valuable because of enzyme help the body absorb basic nutrients at the same time purifying it and used as an antifungal, anti-inflammatory, anti-septic, can be used to accelerate the healing process of wounds [6-8]. By looking at kinds of treatment using aloe vera above, so that was done a deep literature to treatment using aloe vera in dealing with health problems
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Aim
Study researching literature that using aloe vera in treatment or therapy to overcome several health body problems
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Methods
Searching article begins of that 10-25 august 2018 by using a search strategy via the CINAHL database, ResearchGate Pubmed, and ScienceDirect which is used keyword AND aloe vera and efficacy AND aloe vera and getting number of articles 4822 in all searching database. Then was checked based on the suitability of the title and abstract reading related to treatment using aloe vera and got as many as 1502 articles. Then continued the selection of articles based on inclusion criteria totaling 305 articles which can be seen in the table one. After the article was collected than a critical appraisal in using format Joanna Briggs Institute (JBI) so that of 305 critical appraisal articles the result of all relevant articles was obtained 12 articles.
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Criteria
Table 1
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Result
Learnt 9 articles found that aloe vera functional to speed up the healing process of wounds into reduce the intensity of pain in the treatment of oral lichen planus, stomatitis, diabetes mellitus, pressure ulcers, venous ulcers, chronic anal fissure, heal burns, burning mouth syndrome, aphthous minor, operation post [9,10]. So that could be said that the content of aloe vera has function to speed up the healing process of wounds and could reduce the intensity of pain which is due to several health body problems. There is one articles that has different to the efficacy of aloe vera, other to speed up the healing process of wounds and reduce the intensity of pain which one aloe vera gel was encapsulated in liposome was used in the healing melasma process. Because the content aloe vera gel has function as a skin pigmentation modifying agent [11]. Literature learn identified from 9 papers that have been done with critical steps so that it can be seen that from 9 articles discussing aloe vera which are managed or extracted in various forms and has different properties. Application in several treatment related to health problems in the forms of gel cream, juice, and solution of follows
Aloe vera gel
Four researching articles identified the efficacy of aloe vera gel no theses there are differences in other of research. Ghafarzadeh dan Etemadi [11] using aloe vera gel extract (AGE) 0,5 % and encapsulated liposomes 1% weight of treatment given to melasma patient with significant results. Sahzad dan Ahmed researching using aloe vera (Aloe Tone JeIR) get which is not purified and has 98% gels used for the treatment of superficial burns and partial with the result that aloe vera get can speed up the healing process of wounds and reduce the intensity of pain. Jornet et al. [12] identify aloe vera barbadensis gel combined and tongue protector it was found that using of aloe vera gel and tongue protector can reduce duration of wound healing to burning mouth syndrome patients. Others researching that reduce pain score and wound size but also accelerates the healing of alpthous stomatitis wound [13].
Aloe vera cream
Three researching articles identified aloe vera which is managed into the form of cream. Researching by [13] was found using cream of aloe vera oil (AVO cream) toward wounds recovery with results with significant improvement results using pain assessment tools (VAS), AVO cream efficacy statistical test scores increased significantly (p<0.001). Rahmani et al. [14] identified the effect of aloe vera cream 0,5% in chronic anal fissure treatment and it was found that using aloe vera cream could reduce pain and accelerate the process of wound healing during chronic anal fissure treatment. Eshghi et al. [14] also identified the using of aloe vera cream into reduce pain and accelerate the process of post-surgery wound healing, post defection (hemorrhoidectomy) and found that aloe vera cream significantly reduced post-surgery pain at 12, 24, 48 hours and 2 weeks post-surgery and patients who is receiving aloe vera cream has decreased when take number two significantly at 24 and 48 hours after hemorrhoidectomy and aloe vera cream significantly helps wound healing to post surgery patient during 2 weeks.
Aloe vera juice and gel
One researching has been done by S Pol et al. [9] identified combination of aloe vera juice and gel in oral symptomatic lichen planus treatment, the said by combining treatment juice and gel in symptomatic oral lichen planus treatment reduce the duration of pain.
Aloe vera solution
Mansouri et al. [10] using aloe vera solution to stomatitis and pain intensity in patient who undergoing chemotherapy procedures and the results were obtained that using of aloe vera solution to reduce the pain of chemotherapy to stomatitis patients are very effective with statistical test results on third day (on the 3rd day)-14th day P : 0.001 (stomatitis intensity) and P: 0.001 (pain intensity).
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Discussion
Pain is common symptoms of many medical problems. Which indicates tissue damage [15]. Pain has several types such as acute pain, chronic pain, migraine, musculoskeletal, post surgery pain, neuropathic pain, nociceptic and others [16]. One of causes of pain is wound caused by various health problems. Most researching literature reviews that discuss the using of aloe vera therapy through skin. Skin is one of organs as a protector which has function as a receiver regulate humidity, temperature modulation [17]. In 9 researching articles conducted by literature review identify ways to speed the wound healing process and reduce intensity of paint from various health problems with treatment using aloe vera. But we can the type or form of aloe vera which given in the treatment is gel, cream, solution, juice and oil which given through skin. Of 9 articles there was only one researching article which investigate treatment with using aloe vera in a type of solution in stomatitis done by gargling. Whereas it is known that one of the most effective absorption of drugs through skin is solution and liquid [18].
All the results of researching conducted by literature review prove that aloe vera is very use full for used to wound healing process. Aloe vera gel topical using is done by application it to skiing as medicine for skin conditions such as burns, psoriasis, wound and can be taken orally (through mouth) for conditions including osteoarthritis, intestinal disease, fever [19]. Pharmacological action of this get has assessed through in vitro and in vivo experiments. Preclinical researching results provide evidence that aloe vera has activity as anti-inflammatory, antirheumatic, anti-bacterial, and hypoglycemic [20]. Health benefits aloe vera include the application in wound healing process, treat burns and given to protection against skin damage from Aloe vera is a plan that can produce X-rays [21]. Aloe vera is a plant that can produce sap and gel. The gel is extracted from the leaves, and this is the most widely used substance as a treatment [22]. Different from traditional treatment modalities, aloe vera will great reduce medical cost intended as complementary therapy [23]. This paper is still limited in research using aloe vera to speed up the wound healing process and reduce intensity of pain from various health problems, in the type of gel, juice, cream, and especially solution and liquid. More researching is needed on the efficacy of aloe vera in curing various health problems [24,25] Table 2.
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Conclusion
The using of treatment that uses aloe vera has been proven to accelerate the wound healing process and reduce pain intensity in some health problems than other comparison groups and also serves as an improvement in the skin pigmentation
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Acknowledgements
We would like to thank to the lectures nursing Universitas Muhammadiyah Yogyakarta who have given support in solving this literature review.
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Clarifying Concepts About Homeopathy
Dear colleagues, it is known by all that the homeopathy has been mistreated and reviled by those from outside and worse still, also for those of inside of that, with its opinions against the medicine and its indifference in the face of science have made it damage. I consider necessary to make some precisions to clarify and to remark some concepts on the homeopathy. Remaining silent without giving to know our ideas and positions, we will never end up influencing positively in the guild and they will follow some per secula seculorum saying incongruities and worse even being inconsistent with the carried out scientific studies, alone to respect to their “ professor-icons “ located in the Middle Ages, what facilitates that the opponents ridicule and anathematize the homeopathy. Let us begin to clarify some of these concepts using in the appropriate moment some paragraphs of the Theses of Wolf, homeopath belonging to the Central Society of Homeopathy of Germany known also as School of Leipzig and whose members were students and the main collaborators of Hahnemann in the foundation of the homeopathy.
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Are we Another Medicine?
It is well known that we are only a medical therapy and not another medicine not even the new medicine school like erroneously thought at that time the own Hahnemann. The same as the surgery, the allopathy, the immunology, etc. is part of the medicine since we know, and we practice a therapeutic one and we don’t apply the medical knowledge in way different to those that are studied in all the Medicine School. That distinguishes the two treatment schools it is the therapy, not the anatomy, physiology, etiology, nosology, compared anatomy, pathological anatomy. We find the knowledge of these matters’ indispensable. We consider the homeopaths that think that this matter is dispensable, as fellows that belong to another school, we won’t share their merits, neither we will appropriate of the critics that receive [1,2].
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Are we Part of the Complementary Medicine?
We consider that with the previous answer it is defined that the homeopathy is not complementary. It could only be so if the other therapeutic consider each other as complementary one of others. It is in any event an option among the grateful medical therapies [3,4].
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Does Have the Organon of the Medicine Written for Hahnemann All the Answers?
We know that it doesn’t have all the answers, the text is only a normative one to guide and to channel the thought. Those who consider the possibility that the Organon is certain and it contains all the answers, it is in the field of the faith and not of the science, and that we know the homeopathy is not a religion. Some critics have specified with justice that many extravagant and exclusive ideas of the Organon were not approved by many homeopaths [1,2].
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Is the Science A Materialistic Tool Which Only Serves to the Allopath’s?
That is another great fallacy which repeats the approach obscurantist. Is it not science the investigations with methodological and experimental rigor carried out by homeopaths in different places? Also, there is a numberless of great scientists that were idealistic E.g. we have Newton and Mendel. We don’t accept the widespread opinion that the medical homeopath can exclude the reflection and trials on the disease, keeping the symptomatic correlation exclusively. To stay in such raw empiricism could be the tomb of the science [1,2].
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Should We or Not to Be Up to Date?
Only an individual without capacity, intelligence and good trial thinks that it he should not be up to date. That attitude is not characteristic of a Doctor. We have the obligation to investigate and to update our knowledge. For some time, the homeopaths have pointed out the tendency of Hahnemann to sustain certain illusions. In a reiterated and independent way, we have announced that we don’t share neither we support the absence of scientific achievements, neither we proclaim that only the empiric behaviors are valid in the art of healing. We are reproached when Hahnemann says that one cannot know the interior cause, neither it is necessary, because alone the dynamic causes exist. For us the material and organic substrate of the symptoms is able to know. They say that it doesn’t interest us the origin and place of the illnesses, but just by the blind and enthusiast critic produces such asseverations as if it were arguments, opposed to the scientific tendency of the homeopathy. To deny completely the investigation of the nature or essence of the illnesses (as Hahnemann inculcates) it is not an article of faith for the homeopaths. The innate desire to investigate the hidden causes, urges to the human mind to make until of the seemingly inscrutable thing, investigation reason [1,2].
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Is the School of Medicine Synonymous of Medicine Allopath’s School?
The Medicine School is only the place where the future doctors are formed, and not another thing is a medicine school. What happens is that unfortunately it has not been possible to incorporate in all them the homeopathy like curricular matter and therefore in almost all, the graduate ones alone know of the allopathic therapeutic. The schools of medicine graduate health professionals who choose the therapy they like to use more.
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Is It or Not the Allopathy Synonymous of Conventional Medicine?
Far of being a medicine, the allopathy is a pharmacotherapy or medication based on the medical biochemistry, a department in the building of the conventional medicine or medical science.
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If we Make A Mistake when Prescribing, Do we Generate A Suppression or Morbid Metastasis?
To affirm this is not more than a platitude from homeopaths that have not been updated in the biophysical investigations of the homeopathic medication and therefore, they have not been able to discern that that is not possible. Authorities like Hahnemann, Dunham, Nash, neither other grate homeopaths suggested in any moment such a blunder [3,4].
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Is It the Activated Water or Another Factor the Responsible for the Pharmacological Activity in the Dil-Agit?
After Jacques Benveniste’s works and the corroboration of the same ones for Luc Montagnier consider that that answer is given for the homeopaths that stay fairly informed on the experimentation in homeopathy, the other homeopaths simply have the obligation of being updated for not being obsolete [3-5].
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Pre And Postoperative Use of Force Platform in Hallux Valgus Surgery-Case Report
We tried to analyze the importance of information’s obtained using a force platform in objective functional assessment of Scarf osteotomy in hallux valgus surgery. The aim was to obtain a normal architecture of the foot by restoring the normal relations of the first ray and the mobility in the first metatarso-phalangeal articulation. We used the AMTI Accu Gate force platform to analyze pre and postoperative force reaction of the foot succeeding Scarf osteotomy of the first metatarsal. Using the Scarf technique, we were able to restore the normal alignment of the forefoot. Data from the force platform revealed modifications of force reaction components of both leg in contrast with the pedobarographic studies. So, it is important that the technique chosen for correction should reestablish the normal parameters in all 3 space planes.
Keywords: Force platform; Scarf; Hallux valgus
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Introduction
Medical literature considering surgery of the important static deformities of the foot present pressure distribution analysis on foot-ground interface [1-3]. We tried to analyze the importance of informations obtained using a force platform in objective functional assessment of Scarf osteotomy in hallux valgus surgery.
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Case Report
We present the case of a 57- years old male patient presenting left hallux valgus grade II and second hammer toe. Clinical exam revealed pain at first metatarso-phalangeal articulation, presence of exostosis on the medial aspect, first phalange of the hallux in valgus and pronated, the distal phalange under the second toe, presence of dorsal dislocation of the metatarso-phalangeal articulation of the second toe, limited mobility of the first toe (flexion 5°, extension 10°).
To correct the deformity, we used the Scarf technique consisting in exostosectomy, lateral arthrolyse, Z osteotomy of the first metatarsal and osteosynthesis with two screws. The aim was to obtain a normal architecture of the foot by restoring the normal relations of the first ray and the mobility in the first metatarso-phalangeal articulation (flexion 20°, extension 40°).
We used the AMTI AccuGate force platform to analyze pre and postoperative force reaction of the foot succeeding Scarf osteotomy of the first metatarsal. The technique consisted in 2 session of measuring the ground reaction force: vertical (Fz), medio-lateral (Fy) and sagittal (Fx); the first session was done 5 weeks before surgery and the second session at 6 weeks postoperative. Every session consisted in 2 phases of 10 valid successive walking on the force platform with the same leg and the same direction of walking.
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Results
Using the Scarf technique, we were able to restore the normal alignment of the forefoot: metatarso-phalangeal angle of 10°, Varus of the first metatarsal of 10°, distal articular angle of the first metatarsal of 0° and the attack angle of the first metatarsal of 20°. The osteotomy was consolidated at 6 weeks.
We eliminated the aberrant values using the Romanovski test. Testing of the values normality was done using Kolmogorov-Smirnov test; the null hypothesis ″distribution is not normal″ was rejected for all data series. The analysis revealed significant postoperative lengthening of center of pressure trajectory for both the left and the right foot. Significant differences (Paired-Samples T Tests, p<0,05) were obtained for the left foot (Fz-p=0,047; duration of stance phase-p=0,000) and the right foot (Fz-p=0,011; Fx-p=0,030; Fy-p=0,023; duration of stance phase-p=0,000) Table 1. Data from the force platform revealed modifications of force reaction components of both leg in contrast with the pedobarographic studies.
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Discussion
Although in the past walking was considered symmetric for both feet, in the present Viel [4] showed that there is an asymmetry in the parameters of the 2 feet, corresponding to the ″propulsion foot″ (the dominant one) and ″amortization foot″. Gagey and Weber [5] established the value of the normal parameters of the feet on studies for the variation of center of pressure in time, but the data bases are incomplete for this time, so more studies are needed. In our case we have succeeded to restore the normal position of this center. Our data suggest that in hallux valgus there are modifications of kinematic parameters of the foot not only on the vertical plane (Fz), but also in anteroposterior (Fx) and lateral planes (Fy). So, it is important that the technique chosen for correction should reestablish the normal parameters in all 3 planes. In our case the correction obtained with Scarf technique was appropriate.
The purpose of the surgical treatment is to obtain a Greek foot and the metatarsal should be lined up according to Maestro [6] criteria. But it is mandatory to reestablish the normal trajectory of the center of pressure, as showed in our study using the force platform. So, we think that in the future orthopaedic surgeons should use data obtained from these platforms to increase their skills and to realize a better correction of forefoot deformities.
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Conflict of Interest
The authors declare no financial interest or any conflict of interest.
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