Essential oils exhibit biostatic and biocidal activities against microorganism due to antimicrobial properties present in them. The seeds oil of African pear (Dacrodes edulis) was studied against fungi isolated from stored grains. The oil was extracted with soxhlet extractor and antifungal activity was determined using standard method Molecular identification of the fungus showed similar homology with previous isolate. Oil showed static effect as the level of activity decreased as the number of days increased (25.33±0.88,2/3days; 18.00±0.57, 4/5days and 0.00 7th day). Physiochemical and phytochemical parameters showed that D. edulis seed oil has high quality as perioxide value (12.40±0.16mEq/kg) and acid value (1.99±1.08(mgKOH/g)) were within the recommended values of oils. Saponification values were high (190-206mgKOH/g) though iodine value was low. Phytochemical result of D. edulis seed powder included presence of saponins, terpenoids, cardiac glycosides, alkaloids and other compounds. Dacrydes edulis seed oils could be used as biopreservative on stored grains since it was potent against fungi that spoil stored grains. Further phytochemical analysis could reveal the compounds within oil that had the antifungal property.