Aug 3, 2023
S. aureus at 40x after Gram staining
seen from United States
seen from Türkiye
seen from Malaysia
seen from Netherlands
seen from Taiwan
seen from Türkiye
seen from China
seen from Kazakhstan
seen from United States
seen from United States
seen from Kazakhstan
seen from Taiwan
seen from China
seen from United States
seen from United States
seen from United States
seen from United States
seen from United States
seen from United States
seen from Canada
S. aureus at 40x after Gram staining
The banana (Musa paradisiaca) exocarps have been a substantial source for maintaining human health. Banana peel extracts for antimicrobial properties can be of great significance in therapeutic treatments. This study aimed to evaluate the antimicrobial activity of banana exocarps among the selected pathogens. The collected data on antimicrobial assay were presented in means and the standard error of the mean was properly calculated for the zones of inhibition measured for the experiment. Among the test microorganisms, only the Gram-positive bacteria were sensitive to the ethanolic extracts of Lacatan (Musa acuminata C.) and Cardaba (Musa acuminata balbisiana) with the diameter zone of inhibition ranging from 15.30±3.72mm to 15.35±2.13 mm. The ethanolic extract of two varieties of banana exocarps showed various inhibitory effects against several microbial isolates. Highest inhibitory effect against S. aureus with 15 mm inhibition zone, followed by E. coli with no effect. The ethanol extracts of banana exocarps could be considered a good antimicrobial agent against both Gram-positive and negative bacteria to restore the synthetic medicines in the treatment of diseases caused by these microorganisms. The study suggested that the local tribes or ordinary people could explicitly continue utilizing this banana plant as a source of treatment.
Staphylococcus aureus is a prominent human pathogen that is typically found in a variety of human anatomical positions. It is a Gram-positive, non-motile, and facultative anaerobic bacteria. A study was carried out to determine the prevalence of and antibiotic susceptibility pattern of S. aureus isolated from milk. In Peshawar, 100 samples of cow milk were collected from various farms and processed. S. aureus was isolated and identified by inoculation onto Mannitol Salt Agar, gram staining and biochemical analysis. The Kirby Bauer disc diffusion method was used to assess the isolated S. aureus strains for antibiotic susceptibility. By measuring zone sizes and interpreting the data in accordance with predetermined standards. S. aureus prevalence was identified and its patterns of antibiotic susceptibility were evaluated in samples of cow milk. S. aureus was found in 32 of the total 100 samples. Penicillin showed 100% full resistance, followed by Vancomycin (81.2%), Tetracycline (78.1%), and Erythromycin (75%), according to an antimicrobial susceptibility analysis that revealed substantial resistance trends. Contrarily, Gentamicin (84.3%), Trimethoprim-Sulfamethoxazole (75%), Chloramphenicol (59.3%), and Cefoxitin (56.2%) showed the lowest efficacy levels, whereas Ciprofloxacin (90%), which had the highest efficacy. In conclusion, S. aureus was detected in 32% of the 100 samples. Resistance to key antibiotics like Penicillin and Vancomycin was pronounced, highlighting the need for improved farm hygiene. Ciprofloxacin’s efficacy suggests its potential use, while overall, maintaining stringent sanitation practices is crucial for ensuring safe dairy products.
Staphylococcus aureus is a prominent human pathogen that is typically found in a variety of human anatomical positions. It is a Gram-positive, non-motile, and facultative anaerobic bacteria. A study was carried out to determine the prevalence of and antibiotic susceptibility pattern of S. aureus isolated from milk. In Peshawar, 100 samples of cow milk were collected from various farms and processed. S. aureus was isolated and identified by inoculation onto Mannitol Salt Agar, gram staining and biochemical analysis. The Kirby Bauer disc diffusion method was used to assess the isolated S. aureus strains for antibiotic susceptibility. By measuring zone sizes and interpreting the data in accordance with predetermined standards. S. aureus prevalence was identified and its patterns of antibiotic susceptibility were evaluated in samples of cow milk. S. aureus was found in 32 of the total 100 samples. Penicillin showed 100% full resistance, followed by Vancomycin (81.2%), Tetracycline (78.1%), and Erythromycin (75%), according to an antimicrobial susceptibility analysis that revealed substantial resistance trends. Contrarily, Gentamicin (84.3%), Trimethoprim-Sulfamethoxazole (75%), Chloramphenicol (59.3%), and Cefoxitin (56.2%) showed the lowest efficacy levels, whereas Ciprofloxacin (90%), which had the highest efficacy. In conclusion, S. aureus was detected in 32% of the 100 samples. Resistance to key antibiotics like Penicillin and Vancomycin was pronounced, highlighting the need for improved farm hygiene. Ciprofloxacin’s efficacy suggests its potential use, while overall, maintaining stringent sanitation practices is crucial for ensuring safe dairy products.
Fuente: https://twitter.com/urmibajpai/status/1279803088145006593?s=09
Antibacterial Activity of Mulinum spinosum Extracts against Slime-producing Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus Isolated from Nasal Carriers: An Overview | Chapter 6 | Theory and Applications of Microbiology and Biotechnology Vol. 3
Nasal carriers of Staphylococcus aureus are important reservoirs with risk of developing endogenous infections or transmitting infections to susceptible individuals. Methicillin-resistant S. aureus (MRSA) are associated with higher rates of treatment failure. Some strains of S. aureus produce slime which is believed to make the microorganisms more resistant to antibiotics and host defenses. The antibacterial activity of ethyl acetate: n-hexane (EtOAc:HEX) extracts of Mulinum spinosum (5:95% EtOAc:HEX, 50:50% EtOAc:HEX, 70:30% EtOAc:HEX and mix 20:80/30:70% EtOAc:HEX, 50:50/70:30/100:0% EtOAc:HEX) were assayed against 3 slime-producing S. aureus strains and 2 MRSA strains isolated from nasal carriers. S.aureus ATCC 35556 slime-producing strain and MRSA ATCC 43300 strain were used as controls. The extracts were prepared using flash chromatography. M. spinosum 5:95% AcOEt:HEX showed antibacterial effect against all slime-producing strains (MIC:500 µg/ml) and the highest activity against MRSA strains (MIC:500 to 1000 µg/ml). All M.spinosum extracts assayed were active against slime-producing S. aureus and MRSA at doses between 500 and 4000 µg/ml. Both, slime-producing S. aureus and MRSA are highly contagious and hardly eradicated by antibiotic therapies. So, there is an increasing need to find new substances with the ability to inhibit these strains Author(s) Details Echenique Daniela Department of Biochemistry and Biological Sciences, National University of San Luis, San Luis, 5700, Argentina. Chiaramello Alejandra Department of Chemistry, National University of San Luis, San Luis, 5700, Argentina. Mattana Claudia Department of Biochemistry and Biological Sciences, National University of San Luis, San Luis, 5700, Argentina Dr. Alcaráz Lucía Department of Biochemistry and Biological Sciences, National University of San Luis, San Luis, 5700, Argentina Laciar Analía Department of Biochemistry and Biological Sciences, National University of San Luis, San Luis, 5700, Argentina Satorres Sara Department of Biochemistry and Biological Sciences, National University of San Luis, San Luis, 5700, Argentina.
View Book: -
http://bp.bookpi.org/index.php/bpi/catalog/book/154
Check out this time-lapse video that shows a superbug, once thought of as static or non-motile, exhibiting signs of active motility.
Scientists can prove, for the first time, that S. aureus may be able to move independently, which could have major implications for treating future infections caused by the once difficult-to-treat, antibiotic-resistant MRSA.
Read more about this finding here.