Larrymyeggo's blog

Hi everyone, this is Larrymyeggo. I will publish information about milk and milk powder every week.

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How to use the microplate? And What to pay attention to?

Nowadays, most of the microtiter plates used in laboratories are detachable, which can be more convenient for testing. Now the commonly used are basically 96-well microtiter plates. Although the microtiter plates made by different manufacturers are similar on the whole, they will be different in some small details, such as some structures. , This is mainly because of the need to cooperate with different microplate readers.

How to use the microplate:

1. Add sample: add the sample diluent to the coating plate, dilute the serum to be tested with PBS or physiological saline at a ratio of 1:20, then add 10ul to the reaction well of the ELISA plate.

2. Add control: add 3 wells of negative control, 1 well of positive control, and 100ul of control serum.

3. Incubate: shake the reaction plate to mix the sample thoroughly, and place it in a 37°C incubator or water bath for 20 minutes.

4. Wash the plate: Dilute 15ml of the washing solution to a volume of 300ml with distilled water. When washing by hand, you should pour out the contents of the wells of the reaction plate, fill the position of the wells with the washing solution, and then leave it for 30 seconds before shaking it off vigorously. Repeat this action 5 times and then begin to pat dry. Machine washing needs to be carried out 5 times. Each hole is filled with 200ul of washing liquid or can be filled up, and then it is absorbed for 30 seconds and then patted dry.

5. Add enzyme-labeled working solution: add 100ul of enzyme-labeled working solution to each well, place it in a 37°C incubator, and wash the plate 5 times after 20 minutes of reaction. The washing operation is the same as step 4 above.

6. This is the last step of the reaction, which is the color development and termination of the reaction: add 50ul of the substrate to the reaction well, and keep it from light for 10 minutes at 37°C. Add 50ul of stop solution to each well and mix well to stop the reaction.

The use of the ELISA plate needs to be carried out under sterile conditions. Disinfection and cleaning must be done carefully before and after use of the microtiter plate, but it should be noted that some microtiter plates need to be carefully read the instructions before disinfection to see if high temperature sterilization is possible.

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Misunderstandings about breakfast milk

     "Pure milk" is often used as "breakfast milk". Milk is a necessary choice for many people's breakfast, but many people confuse "pure milk" and "breakfast milk", in fact, the ingredients and nutrients are different. Pure milk is just fresh milk, and the ingredients of breakfast milk include milk, water, malt extract, peanuts, egg powder, oats, stabilizers, iron fortifiers, zinc fortifiers, etc. The protein content of breakfast milk is generally more than 2.3%. The protein content of pure milk is usually between 2.9%-3.1%. Individuals should pay attention to different choices when eating, and adjust the corresponding food mix to be more beneficial to health.

　　 "Leftover breakfast". Leftovers, or leftovers for fried rice, leftovers for cooking noodles, etc. Many family "mothers" will cook more when cooking dinner, and cook fried rice for their children and family the next morning, or take the leftovers. Heat it up. This kind of breakfast is convenient to make, rich in content, basically the same as a regular meal, and is generally considered to be nutritionally comprehensive.

　　Western breakfast. This high-calorie breakfast can easily lead to obesity, and long-term use of fried foods can also be harmful to the body. Use Western-style fast food for breakfast, lunch and dinner must eat low-calorie food. In addition, this Western-style breakfast has the problem of unbalanced nutrition, with relatively high calories, but it often lacks nutrients such as vitamins, minerals, and fiber. If you choose western-style fast food for breakfast, you should add fruit or vegetable soup to maintain a balanced nutrition and ensure the intake of various nutrients.

　　 Eat excessively nutritious breakfast. Many people are aware of the importance of breakfast and choose breakfast foods as much as possible, and eat a lot of high-protein, high-calorie, high-fat foods, such as cheese, burgers, fried, and fried foods. But an overly nutritious breakfast will only increase the burden on the stomach and intestines, which is harmful to the body. In the early morning, the spleen of the human body is sleepy and sluggish. Excessive dietary nutrients will exceed the digestive capacity of the gastrointestinal tract. Food is not suitable for digestion and absorption. Over time, the digestive function will decline, leading to gastrointestinal diseases and obesity.

　　 Parents must be cognizant of that foods should be nutritionally balanced when preparing breakfast for their children.

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The basic principle of immune colloidal gold

Wes fully automated protein expression quantitative analysis system

Colloidal gold is a commonly used labeling technique. It is a new type of immunolabeling technique that uses colloidal gold as a tracer to be applied to antigen and antibody. It has its unique advantages. It has been widely used in various biological researches in recent years. Almost all immunoblotting techniques used in the clinic use its markers. At the same time, it may be used in flow cytometry, electron microscopy, immunology, molecular biology and even biochips.

Faulk and Taytor introduced colloidal gold into immunochemistry in 1971. Since then, immunocolloidal gold technology has been increasingly used in various fields of biomedicine as a new immunological method. The current applications in medical tests are mainly immunochromatogra-phy and Dot-immuogold filtration assay DIGFA, which are used to detect HBsAg, HCG and anti-double-stranded DNA antibodies, etc., with simple , Fast, accurate and pollution-free.

1. The basic principles of immune colloidal gold technology

Colloidal gold is made of chloroauric acid (HAuCl4) under the action of reducing agents such as white phosphorus, ascorbic acid, sodium citrate, tannic acid, etc., which can be polymerized into gold particles of a certain size, and become a stable colloidal state due to static electricity. It forms a negatively charged hydrophobic glue solution, which becomes a stable colloidal state due to static electricity, so it is called colloidal gold. Colloidal gold is negatively charged in a weak alkaline environment and can form a firm bond with the positively charged groups of protein molecules. Since this bond is electrostatic, it does not affect the biological properties of the protein.

In addition to binding to proteins, colloidal gold can also bind to many other biological macromolecules, such as SPA, PHA, ConA, etc. According to some physical properties of colloidal gold, such as high electron density, particle size, shape and color response, coupled with the immune and biological properties of the conjugate, colloidal gold is widely used in immunology, histology, pathology and cells Biology and other fields.

Colloidal gold labeling is essentially a coating process in which polymers such as proteins are adsorbed onto the surface of colloidal gold particles. The adsorption mechanism may be the negative charge on the surface of the colloidal gold particles, which forms a firm bond with the positively charged groups of the protein due to electrostatic adsorption. The reduction method can be used to conveniently prepare colloidal gold particles of various sizes, that is, different colors, from chloroauric acid. This spherical particle has a strong adsorption function for protein, and can bind non-covalently with Staphylococcus A protein, immunoglobulin, toxin, glycoprotein, enzyme, antibiotic, hormone, bovine serum albumin polypeptide conjugate, etc. Therefore, it becomes a very useful tool in basic research and clinical experiments.

Immunogold labelling technology (Immunogold labelling techique) mainly utilizes the high electron density of gold particles. At the junction of gold-labeled protein, dark brown particles can be seen under the microscope. When these labels aggregate in large amounts at the corresponding ligands, Red or pink spots are visible to the naked eye, so it is used in qualitative or semi-quantitative rapid immunoassay methods. This reaction can also be amplified by the deposition of silver particles, which is called immunogold-silver staining.

2. Commonly used immune colloidal gold detection technology

(1) Immunocolloidal gold microscope staining method

Cell suspension smears or tissue sections can be stained with colloidal gold-labeled antibodies, or on the basis of colloidal gold labeling, with silver developing solution to enhance the labeling, so that the reduced silver atoms are deposited on the surface of the labeled gold particles. Can significantly enhance the sensitivity of colloidal gold labeling.

Immunogold electron microscopy staining

Colloidal gold-labeled antibodies or anti-antibodies can be combined with negatively stained virus samples or tissue ultrathin sections, and then negatively stained. It can be used for the observation of virus morphology and virus detection.

(2) Dot immunogold percolation method

Use a microporous filter membrane (such as a membrane) as a carrier, first spot the antigen or antibody on the membrane, add the sample to be tested after sealing, and then use the colloidal gold-labeled antibody to detect the corresponding antigen or antibody.

(3) Colloidal gold immunochromatography

The specific antigen or antibody is fixed on the membrane in a strip shape, and the colloidal gold labeling reagent (antibody or monoclonal antibody) is adsorbed on the binding pad. When the sample to be tested is added to the sample pad at one end of the test strip, the capillary action Move forward, dissolve the colloidal gold labeling reagent on the binding pad and react with each other, and then move to the fixed antigen or antibody area, the conjugate of the test substance and the gold labeling reagent will specifically bind with it and be retained and aggregate On the test strip, the color result can be observed by naked eyes. This method has now been developed into a diagnostic test strip, which is very convenient to use.

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Ways to avoid buying fake milk powder:

1. Choose foreign milk powder imported from formal channels

Online shopping overseas for milk powder is very risky and it is difficult to buy the real product. If you want to choose a foreign milk powder brand, you must choose foreign milk powder imported from formal channels, because such milk powder is produced in accordance with Chinese standards. If you insist on choosing Haitao or foreign purchasing agents, please try your best to choose an e-commerce platform with guaranteed quality and safety.

2. Purchase domestic milk powder from regular sales channels

There are many ways to buy milk powder, and domestic consumers still buy domestic milk powder from regular channels. Buying through informal channels not only fails to protect the rights and interests of consumers, but it is also easy to buy fakes.

3. Once you find fake milk powder, you should report it immediately

In order to protect the legitimate rights and interests of consumers, once a fake milk powder is discovered, it should be reported immediately and the wrongdoer should be severely punished to prevent more people from being deceived.

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How to distinguish true and fake milk powder:

1. The barcode on the package Now all products have barcodes, and the beginning of the barcode is different in different countries. Pay attention to the beginning of the barcode. Different origins correspond to different numbers such as: USA, Canada 00-09, Holland 87, Australia 93 and so on. If you buy Australian milk powder and the barcode does not start with 93, it is not true.

2. Milk powder packaging The packaging of formal manufacturers is also very formal and has certain standards. The pattern is clear and the text is clear. The introduction of product information is more specific, including formulas.

3. Anti-counterfeiting code There are usually anti-counterfeiting marks or anti-counterfeiting numbers on the packaging, and so on, the authenticity can be confirmed by query.

4. Color Take a look at the color, real milk powder is natural milky white, a little yellowish. 

5. Dissolution speed Put the milk powder into the cup and rinse with cold boiling water. The real milk powder needs to be stirred to dissolve into a milky turbid liquid; the fake milk powder can automatically dissolve or precipitate without stirring.

6. Boiled water form When flushed with hot boiled water, the real milk powder forms a suspended matter and floats up, which will stick to the spoon at the beginning of stirring; the adulterated milk powder dissolves quickly without the fragrance and color of natural milk.

7. Feel

It is not enough just to pass these. You can twist the milk powder through the bag with your hand. The real milk powder is very delicate and will make a "squeaking" sound; the fake milk powder will be rougher and the particles will be larger. The real milk powder makes a very small sound when twisted, and the fake milk powder will "rustle".

8. Taste Put a little milk powder into your mouth to taste. The real milk powder is delicate and sticky, easy to stick to the teeth, tongue and upper jaw. It dissolves quickly and has a sugar-free sweetness (except for sugared milk powder); fake milk powder dissolves quickly in the mouth. Non-sticky, strong sweetness.

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Is milk can be eaten  with antibiotics?

There are several aspects to the application of antibiotics. The first one is to prevent infection. For example, before surgery, some patients will be given antibiotics by doctors to prevent infection, but most of the conditions are curative. Many of the symptoms caused by bacterial infections can be treated by taking antibiotics or infusing antibiotics. Can antibiotic drugs be eaten with milk?

It is not recommended to drink antiviral drugs together with pure milk, because dairy products contain more protein and sodium ions. Sodium ions can be converted into complex ions or chelates with tetracyclines and isoniazid, which are not easy to be digested and absorbed by the digestive tract, and weaken the antibacterial effect; sodium ions, polyphosphates and potassium thiocyanate can be converted into The less soluble calcium carbonate and calcium bicarbonate settle and reduce their efficacy.

(It is not necessary to drink fruit juice. The salicylic acid contained in fruit juice (especially fresh fruit juice) speeds up the melting of antibiotics, which not only reduces the potency of the medicine, but may also be transformed into hazardous substances and increase toxic side effects).

When taking the following medicines, the time interval between taking and taking milk is not suitable to be too short, let alone taking it with milk:

Antibiotics: including tetracycline, oxytetracycline, doxycycline, etc. Because the sodium ions in this type of medicine and cow's milk will produce complex ions in the stomach and intestines, which greatly reduces their digestion and absorption, reduces their efficacy, and is even completely ineffective. In addition, the calcium content of cow's milk itself, used for delivery of pills or other medicines, is very easy to cause thickening of the gastrointestinal tract, causing the efficacy of the drug to be ineffective. In severe cases, it can turn into gallstones and kidney stones.

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Benefits of probiotics

Digestive system diseases

Infectious diarrhea (caused by viruses, bacteria or parasites), antibiotic-related diarrhea, irritable bowel syndrome, inflammatory bowel disease (IBD), etc.

The most studied probiotic function: There is strong evidence that certain probiotics can help treat diarrhea caused by infection or antibiotics. Feeding certain probiotics to babies can shorten the course of the disease; probiotics help balance the "good and bad" bacteria in the intestines and improve the microbial balance; help reduce gas, bloating and diarrhea; relieve constipation.

Allergic disease

Atopic dermatitis (eczema) and allergic rhinitis (hay fever).

Certain probiotic strains can reduce the severity of eczema in children and infants. Both pregnant women taking probiotics and infant feeding milk containing probiotics can improve infant eczema. Some probiotics can also reduce inflammation in babies allergic to milk protein.

common cold

It can reduce the incidence of common colds, such as fever, cough, and runny nose.

Probiotics may help strengthen the immune system and inhibit the growth of harmful intestinal bacteria. Some probiotics have been shown to promote the production of natural antibodies in the body, and they can also enhance immune cell function.

Infant colic

The cause of colic is not yet known, but some experts believe that it may be related to a small amount of gas producing bacteria and a certain type of bacteria in the baby’s intestines. Probiotics can reduce colic symptoms by balancing beneficial bacteria in the intestine.

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These common diseases do not require antibiotics

1. Chronic pharyngitis

If acute pharyngitis is not treated in time, it will turn into chronic pharyngitis, with symptoms such as dry vomiting and itchy throat, but generally there is no germs, but the pathological changes due to chronic inflammation of the pharyngeal mucosa, such as mucosal hyperemia, swelling, hypertrophy, and dryness , Thinning, shrinking, etc. Unless there is a bacterial infection, such as pus spots in the pharynx, fever and other symptoms, antibiotics are needed and follow the doctor's advice.

2. Gynecological inflammation

Chronic pelvic inflammatory disease and chronic cervicitis do not need to use antibiotics. Only when acute inflammation occurs, antibiotics are needed, and the hospital must select drugs based on bacterial culture and drug sensitivity test. For vaginitis, vulvitis, etc., only trichomonal vaginitis and bacterial vagina require antibiotics, and the others do not require antibiotic treatment.

3. Prostatitis

According to multiple studies, only about 5%-10% of chronic prostatitis have a clear bacterial infection and require oral antibiotic treatment. In addition, the antibiotic treatment of chronic prostatitis should be based on the lower urinary tract bacterial culture and drug sensitivity test, rather than taking antibiotics randomly because you feel uncomfortable with inflammation.

4. Diarrhea

Diarrheal diseases are divided into infectious and non-infectious. Antibiotics are not necessary for non-infectious diarrhea. Antibiotics are not suitable for cold-damp diarrhea caused by viruses, food-injury diarrhea caused by overeating, and spleen deficiency diarrhea caused by spleen deficiency.

5. Cold (upper respiratory tract infection)

Ninety percent of colds are caused by viral infections. Antibiotics have no effect on the virus and have no therapeutic effect. A cold caused by the virus lasts about 5-7 days, and most patients can heal on their own. If the symptoms persist, or are accompanied by high fever or asthma, seek medical advice promptly. Even if a cold is combined with a bacterial infection, the doctor prescribes antibiotics, and the next cold has the same symptoms, you should not use the medicine left over from the last time, because the infected bacteria may not be the same type.

6. Otitis media

When acute suppurative otitis media manifests with symptoms such as high fever and vomiting, antibiotics must be prescribed by the doctor. But whether to use antibiotics for chronic otitis media, you should follow your doctor's advice. If you feel that your ears are inflamed and you use antibiotics irregularly, you may increase your risk of resistance.

7. Mumps

Mumps usually occurs in children and adolescents. Common fever, headache, sore throat, poor appetite, vomiting, etc., and parotid gland swelling and pain for several hours. Mumps is caused by virus invasion, so general antibiotics and sulfa drugs are ineffective.

8. Sinusitis

Sinusitis can be caused by bacterial or viral infections. It is difficult to distinguish between patients and should be judged by a doctor. Approximately one-third of patients with sinusitis will develop secondary bacterial infections and require symptomatic treatment with antibiotics.

9. Gastritis

Some gastritis is infectious inflammation, such as caused by Helicobacter pylori or other bacteria, but the removal of Helicobacter pylori cannot be treated with general antibiotics, it is very stubborn; and if it is caused by physical and chemical factors, such as long-term overheating, Rough food, alcohol, drugs, etc., taking antibiotics will not be effective.

10. Urinary tract infection

Acute cystitis is a type of lower urinary tract infection. It is common in women. Frequent urination, urgency, painful urination, or hematuria or fever may occur. Urinary tract infections need to find out the relevant bacteria, and then carry out targeted antibiotic treatment.

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1 meatball = 10 kinds of additives?

When eating hot pot, of course, all kinds of delicious meatballs are indispensable, such as pork balls, beef balls, chicken balls, fish balls, shrimp balls... you can't stop at all!

But do you know? There may be no meat in the meatballs!

The question is, if there is no meat in the meatballs, what is there?

In fact, "meatballs" has little or no meat . Although there is no meat, they taste almost like meat.

If you eat meatballs with little or no meat, it may be a bionic food. Most of these meatballs are now on the market.

Bionic food is the use of food technology to simulate the nutrition, flavor or shape of natural food with ordinary food. At present, the most commonly used bionic foods in the food industry are bionic meat products and bionic marine foods.

Common bionic meat products are made by mixing soy protein powder and starch in proportions, adding appropriate auxiliary materials, and then extruding into a meat-like product.

Regarding the meat content standard of bionic food, the "Quick Frozen Prepared Food" issued by the Ministry of Commerce stipulates that the content of pork and chicken is ≥10%, the content of beef and lamb is ≥8%, and the other ≥8%.

In supermarkets or vegetable markets, many meatball food packages will indicate the ingredients, but almost no indication of the content of each ingredient (so how much meat is in the meatball is really a mystery).

The beef is five or sixty yuan a catty, and a pack of beef balls is only more than 10 yuan. If the meatballs are all meat, then the business will suffer a miserable loss. 

Meatballs on the market do contain a variety of additives, but as long as food additives are added in accordance with regulations, people will have little effect on their health after eating them.

However, it is not ruled out that some businesses add substances that are harmful to the human body for profit. Therefore, if you want to buy "safe meat", it is best to buy it at a regular supermarket. In addition, compared with real meat, this bionic meat product has a lower fat content.

The incidence of chronic diseases and obesity is increasing year by year. Eating this kind of bionic meat in moderation can satisfy everyone’s taste and psychological needs of meat, and also reduce the intake of meat and fat, which is positive for weight control.

If you usually eat a lot of meat, are overweight or have "three highs", it is beneficial to eat bionic meatballs instead of meatballs.

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The raw material pigment sodium cyclamate exceeded the standard and the company was fined 20,000 yuan

The National Processed Food Quality Inspection Center issued an inspection report on dried mango products produced by Hainan Hongchen Industry and Trade Co., Ltd. The report showed that Hainan Hongchen Industry and Trade Co., Ltd. produced 150g/pack of mangoes on August 27, 2018. Dry product inspection of sodium cyclamate and lemon yellow does not meet GB2760-2014 "Standard for the Use of Food Additives", the test result is that sodium cyclamate 11.1g/kg, the standard requirement is ≤8.0g/kg; lemon yellow 0.19g/kg, the standard requirement is ≤0.1g/kg. Therefore, the inspection conclusion of the product is unqualified.

After receiving the report, law enforcement officers from the Food and Drug Administration of Ding'an County investigated and dealt with Hainan Hongchen Industry and Trade Co., Ltd. in Dingcheng Town, Ding'an County, Hainan Province, and sent the food inspection report to the relevant person in charge of the company. When the law enforcement officers inspected the company’s production workshop, the production workshop was in a state of waiting for production and no dried mango products were found; the company’s finished product warehouse was not found to stock the dried mango products produced on August 27, 2018. One box (25 kg) of dried mango raw materials in stock was found in the warehouse. Law enforcement officers seized the raw materials on site; no sodium cyclamate (sodium cyclamate, calcium cyclamate) and sodium cyclamate were found in the company. Lemon yellow and its aluminum lake food additives.

In the company's inspection room, law enforcement officers found that there was a factory inspection report for the finished dried mango (specification 150 g/pack) produced on August 27, 2018. Law enforcement officers asked about the inspection results of this batch of dried mango products. The relevant person in charge of the company had objections to the inspection results and applied for re-inspection. After re-inspection, it was determined that the dried mango produced by the company on August 27, 2018 was a substandard product.

Subsequently, the Food and Drug Administration of Ding’an County opened the case to investigate and deal with the case. After investigation, it was found that the batch of unqualified dried mangoes produced by Hainan Hongchen Industry and Trade Co., Ltd. were sold to many supermarkets such as Haikou and Sanya. The value is 2232 yuan. After receiving the inspection report, the company recalled 198 packages of this batch of products, and actually sold 42 packages, resulting in illegal gains of 390.60 yuan.

After the company purchases dried mango raw materials, it only conducts sterilization packaging and does not use sodium cyclamate, lemon yellow and its aluminum lake and related food additives. However, the manufacturer’s qualifications were not requested at the time of purchase, and the qualification certificate of the batch of products could not be provided, only the name and contact information of the purchaser. The company's lax control of raw materials has led to the purchase and use of dried mango raw materials that do not meet food safety standards to produce food.

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EU revises the specification requirements for 2'-fucosyllactose

On March 12, 2019, the official gazette of the European Union reported that on March 11, the European Commission issued the European Union Regulation (EU) 2019/388 on 2'-fucose from Escherichia coli K-12 .The specification requirements for base lactose (2′-fucosyllactose) as a new food ingredient have been revised.

In the relevant items in the list of authorized new food ingredients established by the European Commission Implementation Regulation (EU) 2017/2470, the requirements for the purity of 2'-fucosyllactose and microbiological standards have been revised. The regulation will take effect on the 20th day from the date of publication in the official gazette.

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4-Chlorophenoxyacetic acid detected in mung bean sprouts sold at Ajisen Ramen

On March 27, the Shenzhen Municipal Market Supervision Administration released the latest phase of food safety sampling information. This time, a total of 505 batches of food in the production, circulation, and catering links were sampled, involving 294 business entities in the production, circulation, and catering links. Food indicators are 2575 items. Among them, there are 13 batches of unqualified samples, 11 batches of unqualified food categories, 1 batch of edible agricultural products, and 1 batch of aquatic products; categories of unqualified items are food additives, quality indicators, and non-edible substances.

It is worth noting that among the unqualified products, there is a batch of mung bean sprouts sold by Ajisen Ramen Food Service (Shenzhen) Co., Ltd. Buji Vientiane Branch. The detected value of sodium 4-chlorophenoxyacetate is 0.115 mg/kg. The "Announcement on the Prohibition of the Use of 6-Benzyl Adenine and Other Substances in the Production of Bean Sprouts" (State Food and Drug Administration, Ministry of Agriculture, National Health and Family Planning Commission Announcement No. 11, 2015) requires that the use of bean sprouts is prohibited 4-Chlorophenoxyacetic acid.

4-Chlorophenoxyacetic acid is a plant growth regulator that can promote biosynthesis and biological transfer in plants. It can not only prevent flower and fruit drop, increase fruit production rate, increase fruit growth rate, promote early maturity, and improve plants .For the purpose of quality, it also acts as a herbicide. However, due to its accumulated toxicity to the human body, the state has cancelled its application for production license as a food additive. Bean sprouts are a very common vegetable, and the harmful effects of the accumulation of sodium 4-chlorophenoxyacetate in the human body cannot be ignored.

Sampling information showed that 11 batches of unqualified alcohol were the hardest hit areas for unqualified products. The unqualified items mainly include the detection of sweeteners and unqualified alcohol levels. Among them, cyclamate was detected in 9 batches, sucralose was detected in 1 batch, and the alcohol level was not up to standard in 1 batch.

Sucralose is a white powdery product, easily soluble in water, ethanol and methanol. It is the only functional sweetener produced from sucrose. Its sweetness is 600 times that of sucrose, and the sweetness is pure and sweet. The taste characteristics are very similar to sucrose, no calories, and good stability, especially in aqueous solution. The chemical name of sodium cyclamate is sodium cyclamate. It is a commonly used sweetener. Its sweetness is 30-40 times that of sucrose. As a non-nutritive sweetener, it can be widely used in bread, cakes, beverages, and preparations. In various foods such as wine and preserves. "National Food Safety Standard Food Additives Use Standard" (GB 2760-2014) stipulates that sucralose must not be used in wine, and sodium cyclamate must not be used in distilled wine.

Alcohol is a physical and chemical index of wine, which refers to the number of milliliters of ethanol (alcohol) in 100 milliliters of wine at 20°C, that is, the percentage of volume (capacity). The product's express implementation standard "Whiskey" (GB/T 11857-2008) stipulates that the allowable difference between the measured alcohol content and the label value is ±1.0%vol. The substandard alcohol content of the wine will affect the quality and taste of the wine. In addition, because the actual alcohol content of the product does not match the stated index, it may also constitute deceptive behavior to consumers.

In addition,  aquatic products shall not use sulfur dioxide.

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Cultivate baby's light eating habits from an early age to prevent babies from picky eaters

Breast milk is the best food for babies. This is because breast milk contains a lot of nutrients, which help supplement the baby's nutritional needs. Although breast milk is rich in nutrients, it has a lighter taste, which means that milk powder that can remain the light taste of breast milk can be more easily accepted by the baby when mixed feeding or when the baby is facing a change of milk.

Recently, the Beijing Consumers Association commissioned a research report on infant formula milk powder from the National Non-staple Food Quality Supervision and Inspection Center based on the above flavor characteristics to conduct sensory evaluation of 10 domestic and foreign well-known brand formula milk powders sold in the Chinese market.

In this evaluation, fresh milk was used as the reference material. In the comprehensive evaluation of 7 flavor indicators of light cream, fat, fishy, ​​steamed, vegetable, candy, and metallic flavors, the appearance, differences in flavor, taste and mouthfeel.

From the perspective of lightness, the weaker the typical flavor intensity of infant formula milk powder, the lighter the flavor. Judging from the flavor profile, the two formula milk powders, Ruibuen Organic and Ruibuen Jingpo, are the closest to the fresh milk in their flavor characteristics compared with other milk powders, while the typical flavor characteristics of other milk powders are relatively weaker, it can be said that these two formula milk powders are the most light-flavored formula milk powder in the evaluation.

In fact, when supplementing nutrition for the baby, parents should consider the development of the baby's small taste buds. A light diet not only affects the baby's health, but also determines the baby's eating tendency after adulthood.

Recently, Dr. Dingxiang published an article "Milk tea addiction? can not give up heavy taste? parents may be blamed...", it is mentioned that babies between 2-7 months of age are in a sensitive period of taste. Food taste plays an important role in food preference.

If the baby has a heavy taste for a long time, the taste buds will become dull under strong stimulation, and a stronger taste stimulation is needed to feel the taste. This will cause the baby to gradually develop a bad eating habit of partial and picky eaters, and induce obesity, dental caries, and slow growth. Defects and other problems are not conducive to the healthy growth of the baby.

It can be seen that cultivating the baby's light eating habits and reducing the stimulation of the baby's taste buds will benefit the baby for life.

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Infant formula milk powder with light taste has become the development trend of the industry

As an important ration for babies, infant formula milk powder plays a key role in the baby’s physical and intellectual development.

 In recent decades, in comparison with the gold standard breast milk, infant formula milk powder has been iteratively upgraded in product development. Infant formula milk powder that is light and close to breast milk has become a major trend in the development of the entire industry.

At the same time, more and more parents are advocating refined feeding. When purchasing milk powder for their babies, they prefer to choose breast milk powder without maltodextrin and sucrose to provide natural nutrition for the baby and establish a baby’s diet from an early age.

 Light habits have become a positive parenting consensus.

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How to eat dried mulberries to achieve a hangover effect?

"Suixiju Diet Spectrum" in the Qing Dynasty said that mulberry: "Nourishes the liver and kidneys, consolidates blood, quenches thirst, strengthens joints, relieves alcohol and toxins, expels rheumatism, improves ears and eyesight, and calms the soul."

Eating 3-4 dried mulberries 15 minutes before drinking will increase the capacity of alcohol by 2 to 3 times, and it is not easy to get drunk. There is no hangover even when drunk. The most uncomfortable thing about being drunk is the stomach after vomiting and the headache the next morning. Someone has done an experiment. Drinking after eating dried mulberries will increase the capacity of alcohol, and at the same time it will no longer be uncomfortable after being drunk.

Usage: 30 to 15 minutes before drinking, consume 3-5 dried mulberries, chew directly, and drink about 300ml of water.

Efficacy: Reactivate your abilities in 3-7 days at the earliest, making you feel full of motivation. Getting up early every day will make you feel like you are back to 18 years old. At night, you will obviously feel that your abilities will become stronger and you will not be tired. !

Reminder, mulberries can not only get over hangover, but also have a good nourishing effect. Men insist on soaking 2-3 black mulberries in warm water before going to bed every day, and reactivate your abilities in 3-7 days at the earliest, making you feel full of motivation. 

For more information,click here.

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3 tips for preventing drunkenness

Drinking yogurt before drinking alcohol: yogurt is a drink with extremely nutritious value. Drinking some yogurt before drinking can help prevent drunkenness. Compared with clear water, yogurt is thicker. Drinking it into the stomach will form a protective film, which can reduce damage to the stomach.

Eat more green leafy vegetables: When we drink, alcohol is metabolized by the liver. Excessive consumption will burden the liver and cause a certain degree of damage to the liver. So in the process of drinking, eat more green leafy vegetables. The antioxidants and vitamins in them can protect the liver and help ease the speed of drunkenness.

Eat a few dried mulberries before drinking: Dried mulberries contain active enzymes, which are the best substances for hangover. Since ancient times, humans have not been able to artificially synthesize such substances, which are extremely rare. Friends who like to drink eating some mulberries can effectively protect the stomach and liver from alcohol damage before drinking, and accelerate the decomposition of alcohol by the liver, thereby achieving a hangover effect.

Eating dried mulberries before drinking can prevent alcoholism, especially suitable for people who have headaches and nausea after drinking. The selenium in mulberries is the most important "hepatoprotective factor", which enhances liver function, improves liver antioxidant, detoxification, and anti-toxin ability, accelerates the decomposition of alcohol, and plays a role of anti-alcohol and liver protection.

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The knowledge of drinking milk

After some people drink milk, their stomachs will gurgle, pain and swell , and they will also have symptoms such as diarrhea. This is because milk contains a kind of lactose. After the baby is born, there is lactase in the intestine to break down the lactose in the milk during the breastfeeding stage. The lactose will be fermented and decomposed in the large intestine to produce water, carbon dioxide and lactic acid. Lactic acid is not easily absorbed by the body. Carbon dioxide is a gas, so it causes symptoms such as flatulence, abdominal pain, bowel, and diarrhea.

What should an adult do if this symptom occurs after drinking milk?

1. Be careful to drink less, or drink small amounts for several times, drink half a bottle (120ml) of milk each time, so that after 12 months of insisting on drinking, the activity of lactase in the intestines may increase again. Symptoms of bowel and diarrhea will not occur.

2. Combining milk and eggs is another way. When the milk is heated, put the raw eggs in and stir, and eat them half-cooked. The eggs are most easily digested when they are half-cooked. This way of eating has complementary effects in terms of digestion and nutrition.

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