#antibiogram

[focus-on-pharmacy]

20/11/2017 Today on the Microbiology lab.

[focus-on-pharmacy]

06/11/2017 My antibiogram from microbiology.

[haridiva]

Panduan Penyusunan Antibiogram Rumah Sakit di Indonesia

Pendahuluan Antibiogram merupakan instrumen penting dalam program pengendalian resistensi antimikroba (PPRA) yang menyajikan data pola kepekaan mikroorganisme terhadap antimikroba secara kumulatif dalam periode waktu tertentu. Penyusunan antibiogram yang sistematis dan berbasis bukti ilmiah menjadi kunci keberhasilan penatalaksanaan infeksi empiris dan pengendalian resistensi antimikroba di…

Panduan Penyusunan Antibiogram Rumah Sakit di Indonesia

[oaresearchpaper]

Burns remain a significant public health problem in terms of morbidity, long-term disability and mortality. In India, over 10,00,000 people are moderately or severely burnt every year. Open and large wounds, make burn patients more susceptible to infection. In particular, immunosuppression caused by impaired neutrophil function, cellular and humoral immune system can facilitate multiplication and colonization of burn wounds by different microorganisms. The objective for the study to identify the aerobic bacterial agents responsible for burn wound infection and to study the antibiogram of bacterial isolates. The study was performed in the Department of Microbiology, Mysore Medical College and Research Institute, Mysore, for a period of one year. Wound swabs were collected from the burn wounds, were processed according to standard laboratory procedures to isolate aerobic bacterial pathogens. The isolates were tested for antimicrobial susceptibility and resistance mechanisms by Kirby -Bauer disk diffusion method as per CLSI guidelines. 90 burn patients were included in present study. A total of 114 aerobic bacterial isolates were isolated. Staphylococcus aureus (28.9%) was most common isolate followed by Pseudomonas species (26.3%), Klebsiella species (20.2%), Enterococcus species (7.1%), Enterobacter species, Acinetobacter species and Coagulase negative Staphylococcus (3.5%) each, Escherichia coli and Citrobacter species (2.6%) and Proteus species (1.8%). Antibiogram showed that, Imipenem (86.9%) was most effective drug against Gram negative organisms and vancomycin and linezolid (100%) were effective drugs against Gram positive organisms. It is crucial for every burn unit to determine the specific pattern of burn wound colonization and the antimicrobial resistance pattern. This will enable early treatment with proper empirical systemic antibiotics, thus improving overall infection related morbidity and mortality.

[oaresearchpaper]

To estimate the microbial profile and antibiogram of isolates from diabetic foot ulcer in tertiary care hospital of central India. This is a cross-sectional observational study conducted in patients presenting with diabetic foot ulcer in OPDs and admitted in tertiary care hospital of Chhattisgarh. Foot ulcer grading was done using Wagner’s classification. Samples processing, isolation and identification of positive isolates was done using standard microbiological procedures. Antimicrobial susceptibility was evaluated using CLSI guidelines. Total 30 patients of diabetic foot ulcer were included. Most common age group affected was 31-60 years (66.7%) followed by 61-90 years (26.7%). Male to female ratio was 2:1. Mean duration of diabetes was 3.36 years with minimum duration 10 days and maximum duration 20 years in study patients. According to Wagner’s grading, most common grade of ulcer was grade 1 (33.3%) followed by grade 2 (30%) and 3(30%). Peripheral neuropathy was associated in all cases and 43.3% showed association with hypertension. Most common site of diabetic ulcer was right foot 21 (70%) and dorsum of foot 17 (56.7%). Most common organism isolate was Pseudomonas aeruginosa (30%), followed by E. coli (25%) and Klebsiella pneumoniae (25%). Among Gram positive most common organism isolate was Staphylococcus aureus (15%). Imipenem and Meropenem were found to be effective drugs for Gram-negative organisms. For Gram-positive coverage Gentamycin and cephalosporins was found to be effective. Early diagnosis, prompt patient care and implementation of strict antimicrobial stewardship practices are crucial for treatment of diabetic foot ulcer.

[oaresearchpaper]

Burns remain a significant public health problem in terms of morbidity, long-term disability and mortality. In India, over 10,00,000 people are moderately or severely burnt every year. Open and large wounds, make burn patients more susceptible to infection. In particular, immunosuppression caused by impaired neutrophil function, cellular and humoral immune system can facilitate multiplication and colonization of burn wounds by different microorganisms. The objective for the study to identify the aerobic bacterial agents responsible for burn wound infection and to study the antibiogram of bacterial isolates. The study was performed in the Department of Microbiology, Mysore Medical College and Research Institute, Mysore, for a period of one year. Wound swabs were collected from the burn wounds, were processed according to standard laboratory procedures to isolate aerobic bacterial pathogens. The isolates were tested for antimicrobial susceptibility and resistance mechanisms by Kirby -Bauer disk diffusion method as per CLSI guidelines. 90 burn patients were included in present study. A total of 114 aerobic bacterial isolates were isolated. Staphylococcus aureus (28.9%) was most common isolate followed by Pseudomonas species (26.3%), Klebsiella species (20.2%), Enterococcus species (7.1%), Enterobacter species, Acinetobacter species and Coagulase negative Staphylococcus (3.5%) each, Escherichia coli and Citrobacter species (2.6%) and Proteus species (1.8%). Antibiogram showed that, Imipenem (86.9%) was most effective drug against Gram negative organisms and vancomycin and linezolid (100%) were effective drugs against Gram positive organisms. It is crucial for every burn unit to determine the specific pattern of burn wound colonization and the antimicrobial resistance pattern. This will enable early treatment with proper empirical systemic antibiotics, thus improving overall infection related morbidity and mortality.published by the International journal of Microbiology and Mycology (IJMM)

[asrarblog]

A New Tool to Combat Antimicrobial Resistance – Asrar Qureshi’s Blog Post #657

A New Tool to Combat Antimicrobial Resistance – Asrar Qureshi’s Blog Post #657

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[ijtsrd]

Antibiogram of Staphylococcus Aureus and its Sensitivity to Ocimum Gratissimum Extract

by Komolafe T. O. | Ogunyankin O. G "Antibiogram of Staphylococcus Aureus and its Sensitivity to Ocimum Gratissimum Extract"

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021,

URL: https://www.ijtsrd.com/papers/ijtsrd46333.pdf

Paper URL : https://www.ijtsrd.com/other-scientific-research-area/other/46333/antibiogram-of-staphylococcus-aureus-and-its-sensitivity-to-ocimum-gratissimum-extract/komolafe-t-o

ugcapprovedmanagementjournal, openaccessjournalofmanagement, paperpublicationinmanagement

In this study, clinical isolate of Staphylococcus aureus was subjected to susceptibility tests against commercially available antibiotics and Ocimum gratissimum scent leaf leaf extracts. disc diffusion nmethod was adopted for the antibiotics test while agar well diffusion technique was employed for the antimicrobial screening of the leaf extracts. The result shows that the organism was sensitive to Gentamycin, Zinnacef, Ciprofloxacin and Streptomycin representing 40 of the antibiotics while showing outright resistance to Ampiclox, Amoxicillin and Erythromycin 30 and intermediate results for the rest. Also, the cold water extract of O. gratissimum did not show any activity against the bacterium. However, both hot water extract and ethanol extract of the plant had varying degree of activity against the organism with ethanol extract recording a zone of inhibition of 25.33 mm compared with 32.00 mm recorded for Gentamycin. Furthermore, the minimum inhibitory concentration recorded ofr the extract against the organism were 10 mg ml and 2.5 mg ml respectively for hot water and ethanol extracts. These results indicate that ethanol extract of O. gratissimum leaf is very effective against Staphylococcus aureus and could be used as potential source of natural product for the treatment of infections caused by the organism especially the antibiotic resistant strains.